Flavonoid Phloretin Inhibits Adipogenesis and Increases OPG Expression in Adipocytes Derived from Human Bone-Marrow Mesenchymal Stromal-Cells

Phloretin (a flavonoid abundant in apple), has antioxidant, anti-inflammatory, and glucose-transporter inhibitory properties. Thus, it has interesting pharmacological and nutraceutical potential. Bone-marrow mesenchymal stem cells (MSC) have high differentiation capacity, being essential for maintai...

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Autores principales:	Antonio Casado-Díaz, Ángel Rodríguez-Ramos, Bárbara Torrecillas-Baena, Gabriel Dorado, José Manuel Quesada-Gómez, María Ángeles Gálvez-Moreno
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	phloretin human mesenchymal stem cells adipogenesis obesity bone Nutrition. Foods and food supply TX341-641
Acceso en línea:	https://doaj.org/article/e5c5301bae7249889b0dc2d9fc05dbdb
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spelling	oai:doaj.org-article:e5c5301bae7249889b0dc2d9fc05dbdb2021-11-25T18:37:26ZFlavonoid Phloretin Inhibits Adipogenesis and Increases OPG Expression in Adipocytes Derived from Human Bone-Marrow Mesenchymal Stromal-Cells10.3390/nu131141852072-6643https://doaj.org/article/e5c5301bae7249889b0dc2d9fc05dbdb2021-11-01T00:00:00Zhttps://www.mdpi.com/2072-6643/13/11/4185https://doaj.org/toc/2072-6643Phloretin (a flavonoid abundant in apple), has antioxidant, anti-inflammatory, and glucose-transporter inhibitory properties. Thus, it has interesting pharmacological and nutraceutical potential. Bone-marrow mesenchymal stem cells (MSC) have high differentiation capacity, being essential for maintaining homeostasis and regenerative capacity in the organism. Yet, they preferentially differentiate into adipocytes instead of osteoblasts with aging. This has a negative impact on bone turnover, remodeling, and formation. We have evaluated the effects of phloretin on human adipogenesis, analyzing MSC induced to differentiate into adipocytes. Expression of adipogenic genes, as well as genes encoding OPG and RANKL (involved in osteoclastogenesis), protein synthesis, lipid-droplets formation, and apoptosis, were studied. Results showed that 10 and 20 µM phloretin inhibited adipogenesis. This effect was mediated by increasing beta-catenin, as well as increasing apoptosis in adipocytes, at late stages of differentiation. In addition, this chemical increased <i>OPG</i> gene expression and <i>OPG</i>/<i>RANKL</i> ratio in adipocytes. These results suggest that this flavonoid (including phloretin-rich foods) has interesting potential for clinical and regenerative-medicine applications. Thus, such chemicals could be used to counteract obesity and prevent bone-marrow adiposity. That is particularly useful to protect bone mass and treat diseases like osteoporosis, which is an epidemic worldwide.Antonio Casado-DíazÁngel Rodríguez-RamosBárbara Torrecillas-BaenaGabriel DoradoJosé Manuel Quesada-GómezMaría Ángeles Gálvez-MorenoMDPI AGarticlephloretinhuman mesenchymal stem cellsadipogenesisobesityboneNutrition. Foods and food supplyTX341-641ENNutrients, Vol 13, Iss 4185, p 4185 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	phloretin human mesenchymal stem cells adipogenesis obesity bone Nutrition. Foods and food supply TX341-641
spellingShingle	phloretin human mesenchymal stem cells adipogenesis obesity bone Nutrition. Foods and food supply TX341-641 Antonio Casado-Díaz Ángel Rodríguez-Ramos Bárbara Torrecillas-Baena Gabriel Dorado José Manuel Quesada-Gómez María Ángeles Gálvez-Moreno Flavonoid Phloretin Inhibits Adipogenesis and Increases OPG Expression in Adipocytes Derived from Human Bone-Marrow Mesenchymal Stromal-Cells
description	Phloretin (a flavonoid abundant in apple), has antioxidant, anti-inflammatory, and glucose-transporter inhibitory properties. Thus, it has interesting pharmacological and nutraceutical potential. Bone-marrow mesenchymal stem cells (MSC) have high differentiation capacity, being essential for maintaining homeostasis and regenerative capacity in the organism. Yet, they preferentially differentiate into adipocytes instead of osteoblasts with aging. This has a negative impact on bone turnover, remodeling, and formation. We have evaluated the effects of phloretin on human adipogenesis, analyzing MSC induced to differentiate into adipocytes. Expression of adipogenic genes, as well as genes encoding OPG and RANKL (involved in osteoclastogenesis), protein synthesis, lipid-droplets formation, and apoptosis, were studied. Results showed that 10 and 20 µM phloretin inhibited adipogenesis. This effect was mediated by increasing beta-catenin, as well as increasing apoptosis in adipocytes, at late stages of differentiation. In addition, this chemical increased <i>OPG</i> gene expression and <i>OPG</i>/<i>RANKL</i> ratio in adipocytes. These results suggest that this flavonoid (including phloretin-rich foods) has interesting potential for clinical and regenerative-medicine applications. Thus, such chemicals could be used to counteract obesity and prevent bone-marrow adiposity. That is particularly useful to protect bone mass and treat diseases like osteoporosis, which is an epidemic worldwide.
format	article
author	Antonio Casado-Díaz Ángel Rodríguez-Ramos Bárbara Torrecillas-Baena Gabriel Dorado José Manuel Quesada-Gómez María Ángeles Gálvez-Moreno
author_facet	Antonio Casado-Díaz Ángel Rodríguez-Ramos Bárbara Torrecillas-Baena Gabriel Dorado José Manuel Quesada-Gómez María Ángeles Gálvez-Moreno
author_sort	Antonio Casado-Díaz
title	Flavonoid Phloretin Inhibits Adipogenesis and Increases OPG Expression in Adipocytes Derived from Human Bone-Marrow Mesenchymal Stromal-Cells
title_short	Flavonoid Phloretin Inhibits Adipogenesis and Increases OPG Expression in Adipocytes Derived from Human Bone-Marrow Mesenchymal Stromal-Cells
title_full	Flavonoid Phloretin Inhibits Adipogenesis and Increases OPG Expression in Adipocytes Derived from Human Bone-Marrow Mesenchymal Stromal-Cells
title_fullStr	Flavonoid Phloretin Inhibits Adipogenesis and Increases OPG Expression in Adipocytes Derived from Human Bone-Marrow Mesenchymal Stromal-Cells
title_full_unstemmed	Flavonoid Phloretin Inhibits Adipogenesis and Increases OPG Expression in Adipocytes Derived from Human Bone-Marrow Mesenchymal Stromal-Cells
title_sort	flavonoid phloretin inhibits adipogenesis and increases opg expression in adipocytes derived from human bone-marrow mesenchymal stromal-cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e5c5301bae7249889b0dc2d9fc05dbdb
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Flavonoid Phloretin Inhibits Adipogenesis and Increases OPG Expression in Adipocytes Derived from Human Bone-Marrow Mesenchymal Stromal-Cells

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