Expression of Phytase gene in transgenic maize with seed-specific promoter 27-kDa γ Zein and constitutive promoter CaMV 35S

Phytase enzymes are applied to animal feed to help animals absorb more nutrients. The use of feed raw materials containing phytase enzymes is expected to reduce the cost of animal feed production. Efforts to increase the phytase content in maize were carried out by improving genetics, in the way of...

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Autores principales:	Ririn Septina Arthasari, Rani Agustina Wulandari, Panjisakti Basunanda
Formato:	article
Lenguaje:	EN ID
Publicado:	Universitas Gadjah Mada 2021
Materias:	animal feed enzyme gene expression Agriculture S Agriculture (General) S1-972
Acceso en línea:	https://doaj.org/article/e5c5338ff5994c19859f7f25643e3941
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spelling	oai:doaj.org-article:e5c5338ff5994c19859f7f25643e39412021-12-03T04:09:31ZExpression of Phytase gene in transgenic maize with seed-specific promoter 27-kDa γ Zein and constitutive promoter CaMV 35S0126-42142527-716210.22146/ipas.63847https://doaj.org/article/e5c5338ff5994c19859f7f25643e39412021-11-01T00:00:00Zhttps://jurnal.ugm.ac.id/jip/article/view/63847https://doaj.org/toc/0126-4214https://doaj.org/toc/2527-7162Phytase enzymes are applied to animal feed to help animals absorb more nutrients. The use of feed raw materials containing phytase enzymes is expected to reduce the cost of animal feed production. Efforts to increase the phytase content in maize were carried out by improving genetics, in the way of assembling transgenic plants containing high phytase content. The 27-kDa γ Zein promoter is a specific promoter that expresses genes in caryopsis, and promoter CaMV 35S is a constitutive promoter that controls gene expression in all tissues and generally does not depend on the growth phase. Transgenic maize was transformed using Agrobacterium tumefacien infection method on maize B104. The reverse transcriptase polymerase chain reaction (RT-PCR) approach was used to examine the expression of phytase genes in leaves, roots, and caryopsis was done 10, 20, and 30 days after pollination (DAP). The phytase enzyme activity test was also carried out by using the colorimetric phosphomolybdate analysis method to see the phytase enzyme activity in unit µg-1. The results showed that the phytase gene in transgenic plants with the 27-kDa γ Zein promoter was highly expressed in maize caryopsis, but in line Z6.10 was also expressed in leaves, while in the CaMV 35S promoter the phytase gene was only expressed on the leaves. Phytase enzyme activity showed that transgenic maize was higher than non-transgenic maize.Ririn Septina ArthasariRani Agustina WulandariPanjisakti BasunandaUniversitas Gadjah Madaarticleanimal feedenzymegene expressionAgricultureSAgriculture (General)S1-972ENIDIlmu Pertanian (Agricultural Science), Vol 6, Iss 3, Pp 148-155 (2021)
institution	DOAJ
collection	DOAJ
language	EN ID
topic	animal feed enzyme gene expression Agriculture S Agriculture (General) S1-972
spellingShingle	animal feed enzyme gene expression Agriculture S Agriculture (General) S1-972 Ririn Septina Arthasari Rani Agustina Wulandari Panjisakti Basunanda Expression of Phytase gene in transgenic maize with seed-specific promoter 27-kDa γ Zein and constitutive promoter CaMV 35S
description	Phytase enzymes are applied to animal feed to help animals absorb more nutrients. The use of feed raw materials containing phytase enzymes is expected to reduce the cost of animal feed production. Efforts to increase the phytase content in maize were carried out by improving genetics, in the way of assembling transgenic plants containing high phytase content. The 27-kDa γ Zein promoter is a specific promoter that expresses genes in caryopsis, and promoter CaMV 35S is a constitutive promoter that controls gene expression in all tissues and generally does not depend on the growth phase. Transgenic maize was transformed using Agrobacterium tumefacien infection method on maize B104. The reverse transcriptase polymerase chain reaction (RT-PCR) approach was used to examine the expression of phytase genes in leaves, roots, and caryopsis was done 10, 20, and 30 days after pollination (DAP). The phytase enzyme activity test was also carried out by using the colorimetric phosphomolybdate analysis method to see the phytase enzyme activity in unit µg-1. The results showed that the phytase gene in transgenic plants with the 27-kDa γ Zein promoter was highly expressed in maize caryopsis, but in line Z6.10 was also expressed in leaves, while in the CaMV 35S promoter the phytase gene was only expressed on the leaves. Phytase enzyme activity showed that transgenic maize was higher than non-transgenic maize.
format	article
author	Ririn Septina Arthasari Rani Agustina Wulandari Panjisakti Basunanda
author_facet	Ririn Septina Arthasari Rani Agustina Wulandari Panjisakti Basunanda
author_sort	Ririn Septina Arthasari
title	Expression of Phytase gene in transgenic maize with seed-specific promoter 27-kDa γ Zein and constitutive promoter CaMV 35S
title_short	Expression of Phytase gene in transgenic maize with seed-specific promoter 27-kDa γ Zein and constitutive promoter CaMV 35S
title_full	Expression of Phytase gene in transgenic maize with seed-specific promoter 27-kDa γ Zein and constitutive promoter CaMV 35S
title_fullStr	Expression of Phytase gene in transgenic maize with seed-specific promoter 27-kDa γ Zein and constitutive promoter CaMV 35S
title_full_unstemmed	Expression of Phytase gene in transgenic maize with seed-specific promoter 27-kDa γ Zein and constitutive promoter CaMV 35S
title_sort	expression of phytase gene in transgenic maize with seed-specific promoter 27-kda γ zein and constitutive promoter camv 35s
publisher	Universitas Gadjah Mada
publishDate	2021
url	https://doaj.org/article/e5c5338ff5994c19859f7f25643e3941
work_keys_str_mv	AT ririnseptinaarthasari expressionofphytasegeneintransgenicmaizewithseedspecificpromoter27kdagzeinandconstitutivepromotercamv35s AT raniagustinawulandari expressionofphytasegeneintransgenicmaizewithseedspecificpromoter27kdagzeinandconstitutivepromotercamv35s AT panjisaktibasunanda expressionofphytasegeneintransgenicmaizewithseedspecificpromoter27kdagzeinandconstitutivepromotercamv35s
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Expression of Phytase gene in transgenic maize with seed-specific promoter 27-kDa γ Zein and constitutive promoter CaMV 35S

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