Quiescin Sulfhydryl Oxidase 2 Overexpression Predicts Poor Prognosis and Tumor Progression in Patients With Colorectal Cancer: A Study Based on Data Mining and Clinical Verification

Background: As a member of the atypical thiol oxidase family, quiescin sulfhydryl oxidase 2 (QSOX2) has been reported to play an important role in several biological processes, but the expression and function of QSOX2 in colorectal cancer (CRC) remains elusive.Methods: The difference of QSOX2 expres...

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Autores principales:	Tao Jiang, Li Zheng, Xia Li, Jia Liu, Hu Song, Yixin Xu, Chenhua Dong, Lianyu Liu, Hongyu Wang, Shuai Wang, Renhao Wang, Jun Song
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	QSOX2 colorectal cancer TCGA TMAs prognosis GSEA Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/e5c6d7e2ef6f4bddb7151667b499880b
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spelling	oai:doaj.org-article:e5c6d7e2ef6f4bddb7151667b499880b2021-11-10T06:26:39ZQuiescin Sulfhydryl Oxidase 2 Overexpression Predicts Poor Prognosis and Tumor Progression in Patients With Colorectal Cancer: A Study Based on Data Mining and Clinical Verification2296-634X10.3389/fcell.2021.678770https://doaj.org/article/e5c6d7e2ef6f4bddb7151667b499880b2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcell.2021.678770/fullhttps://doaj.org/toc/2296-634XBackground: As a member of the atypical thiol oxidase family, quiescin sulfhydryl oxidase 2 (QSOX2) has been reported to play an important role in several biological processes, but the expression and function of QSOX2 in colorectal cancer (CRC) remains elusive.Methods: The difference of QSOX2 expression, and its relationship with clinicopathological features and prognosis in CRC, was analyzed by bioinformatic analysis and validated by clinical CRC specimen cohort. The functional characterization of QSOX2 was detected via in vitro and vivo experiments in CRC cell lines, while the potential signaling pathways were predicted by Gene Set Enrichment Analysis (GSEA).Results: Our data based on bioinformatical analysis and clinical validation demonstrated that the expression of QSOX2 in CRC tissues was significantly upregulated. Additionally, the chi-square test, logistic regression analysis, and Fisher’s exact test showed that QSOX2 overexpression was significantly correlated with advanced clinicopathological parameters, such as pathological stage and lymph node metastasis. The Kaplan–Meier curves and univariate Cox regression model showed that QSOX2 overexpression predicts poor overall survival (OS) and disease-free survival (DFS) in CRC patients. More importantly, multivariate Cox regression model showed that QSOX2 overexpression could serve as an independent factor for CRC patients. In vitro and vivo data showed that the proliferation and metastasis ability of CRC cells were suppressed on condition of QSOX2 inhibition. In addition, GSEA showed that the QSOX2 high expression phenotype has enriched multiple potential cancer-related signaling pathways.Conclusion: QSOX2 overexpression is strongly associated with malignant progression and poor oncological outcomes in CRC. QSOX2 might act as a novel biomarker for prognosis prediction and a new target for biotherapy in CRC.Tao JiangTao JiangLi ZhengLi ZhengXia LiJia LiuHu SongHu SongYixin XuYixin XuChenhua DongChenhua DongLianyu LiuLianyu LiuHongyu WangHongyu WangShuai WangRenhao WangRenhao WangJun SongJun SongFrontiers Media S.A.articleQSOX2colorectal cancerTCGATMAsprognosisGSEABiology (General)QH301-705.5ENFrontiers in Cell and Developmental Biology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	QSOX2 colorectal cancer TCGA TMAs prognosis GSEA Biology (General) QH301-705.5
spellingShingle	QSOX2 colorectal cancer TCGA TMAs prognosis GSEA Biology (General) QH301-705.5 Tao Jiang Tao Jiang Li Zheng Li Zheng Xia Li Jia Liu Hu Song Hu Song Yixin Xu Yixin Xu Chenhua Dong Chenhua Dong Lianyu Liu Lianyu Liu Hongyu Wang Hongyu Wang Shuai Wang Renhao Wang Renhao Wang Jun Song Jun Song Quiescin Sulfhydryl Oxidase 2 Overexpression Predicts Poor Prognosis and Tumor Progression in Patients With Colorectal Cancer: A Study Based on Data Mining and Clinical Verification
description	Background: As a member of the atypical thiol oxidase family, quiescin sulfhydryl oxidase 2 (QSOX2) has been reported to play an important role in several biological processes, but the expression and function of QSOX2 in colorectal cancer (CRC) remains elusive.Methods: The difference of QSOX2 expression, and its relationship with clinicopathological features and prognosis in CRC, was analyzed by bioinformatic analysis and validated by clinical CRC specimen cohort. The functional characterization of QSOX2 was detected via in vitro and vivo experiments in CRC cell lines, while the potential signaling pathways were predicted by Gene Set Enrichment Analysis (GSEA).Results: Our data based on bioinformatical analysis and clinical validation demonstrated that the expression of QSOX2 in CRC tissues was significantly upregulated. Additionally, the chi-square test, logistic regression analysis, and Fisher’s exact test showed that QSOX2 overexpression was significantly correlated with advanced clinicopathological parameters, such as pathological stage and lymph node metastasis. The Kaplan–Meier curves and univariate Cox regression model showed that QSOX2 overexpression predicts poor overall survival (OS) and disease-free survival (DFS) in CRC patients. More importantly, multivariate Cox regression model showed that QSOX2 overexpression could serve as an independent factor for CRC patients. In vitro and vivo data showed that the proliferation and metastasis ability of CRC cells were suppressed on condition of QSOX2 inhibition. In addition, GSEA showed that the QSOX2 high expression phenotype has enriched multiple potential cancer-related signaling pathways.Conclusion: QSOX2 overexpression is strongly associated with malignant progression and poor oncological outcomes in CRC. QSOX2 might act as a novel biomarker for prognosis prediction and a new target for biotherapy in CRC.
format	article
author	Tao Jiang Tao Jiang Li Zheng Li Zheng Xia Li Jia Liu Hu Song Hu Song Yixin Xu Yixin Xu Chenhua Dong Chenhua Dong Lianyu Liu Lianyu Liu Hongyu Wang Hongyu Wang Shuai Wang Renhao Wang Renhao Wang Jun Song Jun Song
author_facet	Tao Jiang Tao Jiang Li Zheng Li Zheng Xia Li Jia Liu Hu Song Hu Song Yixin Xu Yixin Xu Chenhua Dong Chenhua Dong Lianyu Liu Lianyu Liu Hongyu Wang Hongyu Wang Shuai Wang Renhao Wang Renhao Wang Jun Song Jun Song
author_sort	Tao Jiang
title	Quiescin Sulfhydryl Oxidase 2 Overexpression Predicts Poor Prognosis and Tumor Progression in Patients With Colorectal Cancer: A Study Based on Data Mining and Clinical Verification
title_short	Quiescin Sulfhydryl Oxidase 2 Overexpression Predicts Poor Prognosis and Tumor Progression in Patients With Colorectal Cancer: A Study Based on Data Mining and Clinical Verification
title_full	Quiescin Sulfhydryl Oxidase 2 Overexpression Predicts Poor Prognosis and Tumor Progression in Patients With Colorectal Cancer: A Study Based on Data Mining and Clinical Verification
title_fullStr	Quiescin Sulfhydryl Oxidase 2 Overexpression Predicts Poor Prognosis and Tumor Progression in Patients With Colorectal Cancer: A Study Based on Data Mining and Clinical Verification
title_full_unstemmed	Quiescin Sulfhydryl Oxidase 2 Overexpression Predicts Poor Prognosis and Tumor Progression in Patients With Colorectal Cancer: A Study Based on Data Mining and Clinical Verification
title_sort	quiescin sulfhydryl oxidase 2 overexpression predicts poor prognosis and tumor progression in patients with colorectal cancer: a study based on data mining and clinical verification
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/e5c6d7e2ef6f4bddb7151667b499880b
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Quiescin Sulfhydryl Oxidase 2 Overexpression Predicts Poor Prognosis and Tumor Progression in Patients With Colorectal Cancer: A Study Based on Data Mining and Clinical Verification

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