The possible role of PtdIns(4,5)P2 and PtdIns(3,4,5)P3 at the leading and trailing edges of the breast cancer cell line

Introduction: Phosphoinositides play a key role in the regulation of focal adhesions (FAs) turnover during cell adhesion and migration. However, their potential role in FA turnover at leading and trailing edge of cell are not yet fully understood. In this study, we investigate their spatial co-local...

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Autor principal:	Dhurgam AL-Fahad
Formato:	article
Lenguaje:	EN
Publicado:	Emergency Department of Hospital San Pedro (Logroño, Spain) 2021
Materias:	ptdins(4-5)p2 ptdins(3-4-5)p3 focal adhesions spatial co-localistion Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/e5ca0047887a451f89004163fdc88e2a
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spelling	oai:doaj.org-article:e5ca0047887a451f89004163fdc88e2a2021-12-02T15:37:21ZThe possible role of PtdIns(4,5)P2 and PtdIns(3,4,5)P3 at the leading and trailing edges of the breast cancer cell line10.5281/zenodo.44217992695-5075https://doaj.org/article/e5ca0047887a451f89004163fdc88e2a2021-01-01T00:00:00Zhttps://doi.org/10.5281/zenodo.4421799https://doaj.org/toc/2695-5075Introduction: Phosphoinositides play a key role in the regulation of focal adhesions (FAs) turnover during cell adhesion and migration. However, their potential role in FA turnover at leading and trailing edge of cell are not yet fully understood. In this study, we investigate their spatial co-localisation with paxillin directly at leading and trailing edge of MDA-MB-231 breast cancer cell line. Materials and methods: Cell lines and cell culture experiments were done using MDA-MB-231human adenocarcinoma cells. Co-trnasfection and confocal microscopy were performed to visualise phosphoinositides and FAs by using GFP-C1-PLCdelta-PH/Btk-PH-GFP and 3 μɡ paxillin-RFP as biosensors. Then, ImageJ was used to measure co-localisation point between Phosphatidylinositol 4,5-trisphosphate (PtdIns(4,5)P2) or Phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) and paxillin Spearman's rank correlation value was taken between PtdIns(4,5)P2/PtdIns(3,4,5)P3. Results: Our results demonstrate that the spatial co-localistion of PtdIns(4,5)P2 and PtdIns(3,4,5)P3 with FA at leading and trailing age of cell were slightly changed. Conclusions: This suggests that PtdIns(4,5)P2 and PtdIns(3,4,5)P3 play an equal role at the leading and trailing edges of the cancer metastasis through interaction with FA proteins.Dhurgam AL-FahadEmergency Department of Hospital San Pedro (Logroño, Spain)articleptdins(4-5)p2ptdins(3-4-5)p3focal adhesionsspatial co-localistionMedicine (General)R5-920ENIberoamerican Journal of Medicine, Vol 3, Iss 1, Pp 26-32 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ptdins(4-5)p2 ptdins(3-4-5)p3 focal adhesions spatial co-localistion Medicine (General) R5-920
spellingShingle	ptdins(4-5)p2 ptdins(3-4-5)p3 focal adhesions spatial co-localistion Medicine (General) R5-920 Dhurgam AL-Fahad The possible role of PtdIns(4,5)P2 and PtdIns(3,4,5)P3 at the leading and trailing edges of the breast cancer cell line
description	Introduction: Phosphoinositides play a key role in the regulation of focal adhesions (FAs) turnover during cell adhesion and migration. However, their potential role in FA turnover at leading and trailing edge of cell are not yet fully understood. In this study, we investigate their spatial co-localisation with paxillin directly at leading and trailing edge of MDA-MB-231 breast cancer cell line. Materials and methods: Cell lines and cell culture experiments were done using MDA-MB-231human adenocarcinoma cells. Co-trnasfection and confocal microscopy were performed to visualise phosphoinositides and FAs by using GFP-C1-PLCdelta-PH/Btk-PH-GFP and 3 μɡ paxillin-RFP as biosensors. Then, ImageJ was used to measure co-localisation point between Phosphatidylinositol 4,5-trisphosphate (PtdIns(4,5)P2) or Phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) and paxillin Spearman's rank correlation value was taken between PtdIns(4,5)P2/PtdIns(3,4,5)P3. Results: Our results demonstrate that the spatial co-localistion of PtdIns(4,5)P2 and PtdIns(3,4,5)P3 with FA at leading and trailing age of cell were slightly changed. Conclusions: This suggests that PtdIns(4,5)P2 and PtdIns(3,4,5)P3 play an equal role at the leading and trailing edges of the cancer metastasis through interaction with FA proteins.
format	article
author	Dhurgam AL-Fahad
author_facet	Dhurgam AL-Fahad
author_sort	Dhurgam AL-Fahad
title	The possible role of PtdIns(4,5)P2 and PtdIns(3,4,5)P3 at the leading and trailing edges of the breast cancer cell line
title_short	The possible role of PtdIns(4,5)P2 and PtdIns(3,4,5)P3 at the leading and trailing edges of the breast cancer cell line
title_full	The possible role of PtdIns(4,5)P2 and PtdIns(3,4,5)P3 at the leading and trailing edges of the breast cancer cell line
title_fullStr	The possible role of PtdIns(4,5)P2 and PtdIns(3,4,5)P3 at the leading and trailing edges of the breast cancer cell line
title_full_unstemmed	The possible role of PtdIns(4,5)P2 and PtdIns(3,4,5)P3 at the leading and trailing edges of the breast cancer cell line
title_sort	possible role of ptdins(4,5)p2 and ptdins(3,4,5)p3 at the leading and trailing edges of the breast cancer cell line
publisher	Emergency Department of Hospital San Pedro (Logroño, Spain)
publishDate	2021
url	https://doaj.org/article/e5ca0047887a451f89004163fdc88e2a
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The possible role of PtdIns(4,5)P2 and PtdIns(3,4,5)P3 at the leading and trailing edges of the breast cancer cell line

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