GC-TOF/MS-based metabolomics analysis to investigate the changes driven by N-Acetylcysteine in the plant-pathogen Xanthomonas citri subsp. citri

Abstract N-Acetylcysteine (NAC) is an antioxidant, anti-adhesive, and antimicrobial compound. Even though there is much information regarding the role of NAC as an antioxidant and anti-adhesive agent, little is known about its antimicrobial activity. In order to assess its mode of action in bacteria...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Simone Cristina Picchi, Mariana de Souza e Silva, Luiz Leonardo Saldanha, Henrique Ferreira, Marco Aurélio Takita, Camila Caldana, Alessandra Alves de Souza
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e5dea88dbe1f4b19aba27f9461586030
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e5dea88dbe1f4b19aba27f9461586030
record_format	dspace
spelling	oai:doaj.org-article:e5dea88dbe1f4b19aba27f94615860302021-12-02T16:06:43ZGC-TOF/MS-based metabolomics analysis to investigate the changes driven by N-Acetylcysteine in the plant-pathogen Xanthomonas citri subsp. citri10.1038/s41598-021-95113-42045-2322https://doaj.org/article/e5dea88dbe1f4b19aba27f94615860302021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95113-4https://doaj.org/toc/2045-2322Abstract N-Acetylcysteine (NAC) is an antioxidant, anti-adhesive, and antimicrobial compound. Even though there is much information regarding the role of NAC as an antioxidant and anti-adhesive agent, little is known about its antimicrobial activity. In order to assess its mode of action in bacterial cells, we investigated the metabolic responses triggered by NAC at neutral pH. As a model organism, we chose the Gram-negative plant pathogen Xanthomonas citri subsp. citri (X. citri), the causal agent of citrus canker disease, due to the potential use of NAC as a sustainable molecule against phytopathogens dissemination in citrus cultivated areas. In presence of NAC, cell proliferation was affected after 4 h, but damages to the cell membrane were observed only after 24 h. Targeted metabolite profiling analysis using GC–MS/TOF unravelled that NAC seems to be metabolized by the cells affecting cysteine metabolism. Intriguingly, glutamine, a marker for nitrogen status, was not detected among the cells treated with NAC. The absence of glutamine was followed by a decrease in the levels of the majority of the proteinogenic amino acids, suggesting that the reduced availability of amino acids affect protein synthesis and consequently cell proliferation.Simone Cristina PicchiMariana de Souza e SilvaLuiz Leonardo SaldanhaHenrique FerreiraMarco Aurélio TakitaCamila CaldanaAlessandra Alves de SouzaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Simone Cristina Picchi Mariana de Souza e Silva Luiz Leonardo Saldanha Henrique Ferreira Marco Aurélio Takita Camila Caldana Alessandra Alves de Souza GC-TOF/MS-based metabolomics analysis to investigate the changes driven by N-Acetylcysteine in the plant-pathogen Xanthomonas citri subsp. citri
description	Abstract N-Acetylcysteine (NAC) is an antioxidant, anti-adhesive, and antimicrobial compound. Even though there is much information regarding the role of NAC as an antioxidant and anti-adhesive agent, little is known about its antimicrobial activity. In order to assess its mode of action in bacterial cells, we investigated the metabolic responses triggered by NAC at neutral pH. As a model organism, we chose the Gram-negative plant pathogen Xanthomonas citri subsp. citri (X. citri), the causal agent of citrus canker disease, due to the potential use of NAC as a sustainable molecule against phytopathogens dissemination in citrus cultivated areas. In presence of NAC, cell proliferation was affected after 4 h, but damages to the cell membrane were observed only after 24 h. Targeted metabolite profiling analysis using GC–MS/TOF unravelled that NAC seems to be metabolized by the cells affecting cysteine metabolism. Intriguingly, glutamine, a marker for nitrogen status, was not detected among the cells treated with NAC. The absence of glutamine was followed by a decrease in the levels of the majority of the proteinogenic amino acids, suggesting that the reduced availability of amino acids affect protein synthesis and consequently cell proliferation.
format	article
author	Simone Cristina Picchi Mariana de Souza e Silva Luiz Leonardo Saldanha Henrique Ferreira Marco Aurélio Takita Camila Caldana Alessandra Alves de Souza
author_facet	Simone Cristina Picchi Mariana de Souza e Silva Luiz Leonardo Saldanha Henrique Ferreira Marco Aurélio Takita Camila Caldana Alessandra Alves de Souza
author_sort	Simone Cristina Picchi
title	GC-TOF/MS-based metabolomics analysis to investigate the changes driven by N-Acetylcysteine in the plant-pathogen Xanthomonas citri subsp. citri
title_short	GC-TOF/MS-based metabolomics analysis to investigate the changes driven by N-Acetylcysteine in the plant-pathogen Xanthomonas citri subsp. citri
title_full	GC-TOF/MS-based metabolomics analysis to investigate the changes driven by N-Acetylcysteine in the plant-pathogen Xanthomonas citri subsp. citri
title_fullStr	GC-TOF/MS-based metabolomics analysis to investigate the changes driven by N-Acetylcysteine in the plant-pathogen Xanthomonas citri subsp. citri
title_full_unstemmed	GC-TOF/MS-based metabolomics analysis to investigate the changes driven by N-Acetylcysteine in the plant-pathogen Xanthomonas citri subsp. citri
title_sort	gc-tof/ms-based metabolomics analysis to investigate the changes driven by n-acetylcysteine in the plant-pathogen xanthomonas citri subsp. citri
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e5dea88dbe1f4b19aba27f9461586030
work_keys_str_mv	AT simonecristinapicchi gctofmsbasedmetabolomicsanalysistoinvestigatethechangesdrivenbynacetylcysteineintheplantpathogenxanthomonascitrisubspcitri AT marianadesouzaesilva gctofmsbasedmetabolomicsanalysistoinvestigatethechangesdrivenbynacetylcysteineintheplantpathogenxanthomonascitrisubspcitri AT luizleonardosaldanha gctofmsbasedmetabolomicsanalysistoinvestigatethechangesdrivenbynacetylcysteineintheplantpathogenxanthomonascitrisubspcitri AT henriqueferreira gctofmsbasedmetabolomicsanalysistoinvestigatethechangesdrivenbynacetylcysteineintheplantpathogenxanthomonascitrisubspcitri AT marcoaureliotakita gctofmsbasedmetabolomicsanalysistoinvestigatethechangesdrivenbynacetylcysteineintheplantpathogenxanthomonascitrisubspcitri AT camilacaldana gctofmsbasedmetabolomicsanalysistoinvestigatethechangesdrivenbynacetylcysteineintheplantpathogenxanthomonascitrisubspcitri AT alessandraalvesdesouza gctofmsbasedmetabolomicsanalysistoinvestigatethechangesdrivenbynacetylcysteineintheplantpathogenxanthomonascitrisubspcitri
_version_	1718384937880518656

GC-TOF/MS-based metabolomics analysis to investigate the changes driven by N-Acetylcysteine in the plant-pathogen Xanthomonas citri subsp. citri

Ejemplares similares