Different regulation of physiological and tumor angiogenesis in zebrafish by protein kinase D1 (PKD1).

Protein kinase D isoenzymes (PKDs, Prkds) are serine threonine kinases that belong to the CAMK superfamily. PKD1 is expressed in endothelial cells and is a major mediator of biological responses downstream of the VEGFRs that are relevant for angiogenesis such as endothelial cell migration, prolifera...

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Autores principales:	Marcus Hollenbach, Sandra Jasmin Stoll, Kristina Jörgens, Thomas Seufferlein, Jens Kroll
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e5e72074bff74ffabc3ae916657279ce
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spelling	oai:doaj.org-article:e5e72074bff74ffabc3ae916657279ce2021-11-18T07:38:11ZDifferent regulation of physiological and tumor angiogenesis in zebrafish by protein kinase D1 (PKD1).1932-620310.1371/journal.pone.0068033https://doaj.org/article/e5e72074bff74ffabc3ae916657279ce2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23874489/?tool=EBIhttps://doaj.org/toc/1932-6203Protein kinase D isoenzymes (PKDs, Prkds) are serine threonine kinases that belong to the CAMK superfamily. PKD1 is expressed in endothelial cells and is a major mediator of biological responses downstream of the VEGFRs that are relevant for angiogenesis such as endothelial cell migration, proliferation and tubulogenesis in vitro. PKDs also play a critical role in tumor development and progression, including tumor angiogenesis. However, given the plethora of signaling modules that drive angiogenesis, the precise role of PKD1 in both physiological and tumor angiogenesis in vivo has not been worked out so far. This study aimed at dissecting the contribution of PKD1 to physiological blood vessel formation, PKD1 was found to be widely expressed during zebrafish development. As far as physiological angiogenesis was concerned, morpholino-based silencing of PKD1 expression moderately reduced the formation of the intersomitic vessels and the dorsal longitudinal anastomotic vessel in tg(fli1:EGFP) zebrafish. In addition, silencing of PKD1 resulted in reduced formation of the parachordal lymphangioblasts that serves as a precursor for the developing thoracic duct. Interestingly, tumor angiogenesis was completely abolished in PKD1 morphants using the zebrafish/tumor xenograft angiogenesis assay. Our data in zebrafish demonstrate that PKD1 contributes to the regulation of physiological angiogenesis and lymphangiogenesis during zebrafish development and is essential for tumor angiogenesis.Marcus HollenbachSandra Jasmin StollKristina JörgensThomas SeufferleinJens KrollPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e68033 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marcus Hollenbach Sandra Jasmin Stoll Kristina Jörgens Thomas Seufferlein Jens Kroll Different regulation of physiological and tumor angiogenesis in zebrafish by protein kinase D1 (PKD1).
description	Protein kinase D isoenzymes (PKDs, Prkds) are serine threonine kinases that belong to the CAMK superfamily. PKD1 is expressed in endothelial cells and is a major mediator of biological responses downstream of the VEGFRs that are relevant for angiogenesis such as endothelial cell migration, proliferation and tubulogenesis in vitro. PKDs also play a critical role in tumor development and progression, including tumor angiogenesis. However, given the plethora of signaling modules that drive angiogenesis, the precise role of PKD1 in both physiological and tumor angiogenesis in vivo has not been worked out so far. This study aimed at dissecting the contribution of PKD1 to physiological blood vessel formation, PKD1 was found to be widely expressed during zebrafish development. As far as physiological angiogenesis was concerned, morpholino-based silencing of PKD1 expression moderately reduced the formation of the intersomitic vessels and the dorsal longitudinal anastomotic vessel in tg(fli1:EGFP) zebrafish. In addition, silencing of PKD1 resulted in reduced formation of the parachordal lymphangioblasts that serves as a precursor for the developing thoracic duct. Interestingly, tumor angiogenesis was completely abolished in PKD1 morphants using the zebrafish/tumor xenograft angiogenesis assay. Our data in zebrafish demonstrate that PKD1 contributes to the regulation of physiological angiogenesis and lymphangiogenesis during zebrafish development and is essential for tumor angiogenesis.
format	article
author	Marcus Hollenbach Sandra Jasmin Stoll Kristina Jörgens Thomas Seufferlein Jens Kroll
author_facet	Marcus Hollenbach Sandra Jasmin Stoll Kristina Jörgens Thomas Seufferlein Jens Kroll
author_sort	Marcus Hollenbach
title	Different regulation of physiological and tumor angiogenesis in zebrafish by protein kinase D1 (PKD1).
title_short	Different regulation of physiological and tumor angiogenesis in zebrafish by protein kinase D1 (PKD1).
title_full	Different regulation of physiological and tumor angiogenesis in zebrafish by protein kinase D1 (PKD1).
title_fullStr	Different regulation of physiological and tumor angiogenesis in zebrafish by protein kinase D1 (PKD1).
title_full_unstemmed	Different regulation of physiological and tumor angiogenesis in zebrafish by protein kinase D1 (PKD1).
title_sort	different regulation of physiological and tumor angiogenesis in zebrafish by protein kinase d1 (pkd1).
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/e5e72074bff74ffabc3ae916657279ce
work_keys_str_mv	AT marcushollenbach differentregulationofphysiologicalandtumorangiogenesisinzebrafishbyproteinkinased1pkd1 AT sandrajasminstoll differentregulationofphysiologicalandtumorangiogenesisinzebrafishbyproteinkinased1pkd1 AT kristinajorgens differentregulationofphysiologicalandtumorangiogenesisinzebrafishbyproteinkinased1pkd1 AT thomasseufferlein differentregulationofphysiologicalandtumorangiogenesisinzebrafishbyproteinkinased1pkd1 AT jenskroll differentregulationofphysiologicalandtumorangiogenesisinzebrafishbyproteinkinased1pkd1
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Different regulation of physiological and tumor angiogenesis in zebrafish by protein kinase D1 (PKD1).

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