Image-based high-throughput mapping of TGF-β-induced phosphocomplexes at a single-cell level

To improve our ability to monitor cellular responses to e.g. cytokines or drugs, Lönn et al have developed a semi-automated system for large-scale in situ proximity ligation assays (isPLA) in HaCAT keratinocyte cells. Their approach expands the scope for image-based single cell analyses by combining...

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Autores principales: Peter Lönn, Rasel A. Al-Amin, Ehsan Manouchehri Doulabi, Johan Heldin, Radiosa Gallini, Johan Björkesten, Johan Oelrich, Masood Kamali-Moghaddam, Ulf Landegren
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/e5ecdbaa06634e1d97b74de9458d189e
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Sumario:To improve our ability to monitor cellular responses to e.g. cytokines or drugs, Lönn et al have developed a semi-automated system for large-scale in situ proximity ligation assays (isPLA) in HaCAT keratinocyte cells. Their approach expands the scope for image-based single cell analyses by combining observations of protein interactions and modifications with morphological details of individual cells at high throughput.