Image-based high-throughput mapping of TGF-β-induced phosphocomplexes at a single-cell level

To improve our ability to monitor cellular responses to e.g. cytokines or drugs, Lönn et al have developed a semi-automated system for large-scale in situ proximity ligation assays (isPLA) in HaCAT keratinocyte cells. Their approach expands the scope for image-based single cell analyses by combining...

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Autores principales:	Peter Lönn, Rasel A. Al-Amin, Ehsan Manouchehri Doulabi, Johan Heldin, Radiosa Gallini, Johan Björkesten, Johan Oelrich, Masood Kamali-Moghaddam, Ulf Landegren
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/e5ecdbaa06634e1d97b74de9458d189e
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spelling	oai:doaj.org-article:e5ecdbaa06634e1d97b74de9458d189e2021-11-14T12:12:16ZImage-based high-throughput mapping of TGF-β-induced phosphocomplexes at a single-cell level10.1038/s42003-021-02798-42399-3642https://doaj.org/article/e5ecdbaa06634e1d97b74de9458d189e2021-11-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-02798-4https://doaj.org/toc/2399-3642To improve our ability to monitor cellular responses to e.g. cytokines or drugs, Lönn et al have developed a semi-automated system for large-scale in situ proximity ligation assays (isPLA) in HaCAT keratinocyte cells. Their approach expands the scope for image-based single cell analyses by combining observations of protein interactions and modifications with morphological details of individual cells at high throughput.Peter LönnRasel A. Al-AminEhsan Manouchehri DoulabiJohan HeldinRadiosa GalliniJohan BjörkestenJohan OelrichMasood Kamali-MoghaddamUlf LandegrenNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Peter Lönn Rasel A. Al-Amin Ehsan Manouchehri Doulabi Johan Heldin Radiosa Gallini Johan Björkesten Johan Oelrich Masood Kamali-Moghaddam Ulf Landegren Image-based high-throughput mapping of TGF-β-induced phosphocomplexes at a single-cell level
description	To improve our ability to monitor cellular responses to e.g. cytokines or drugs, Lönn et al have developed a semi-automated system for large-scale in situ proximity ligation assays (isPLA) in HaCAT keratinocyte cells. Their approach expands the scope for image-based single cell analyses by combining observations of protein interactions and modifications with morphological details of individual cells at high throughput.
format	article
author	Peter Lönn Rasel A. Al-Amin Ehsan Manouchehri Doulabi Johan Heldin Radiosa Gallini Johan Björkesten Johan Oelrich Masood Kamali-Moghaddam Ulf Landegren
author_facet	Peter Lönn Rasel A. Al-Amin Ehsan Manouchehri Doulabi Johan Heldin Radiosa Gallini Johan Björkesten Johan Oelrich Masood Kamali-Moghaddam Ulf Landegren
author_sort	Peter Lönn
title	Image-based high-throughput mapping of TGF-β-induced phosphocomplexes at a single-cell level
title_short	Image-based high-throughput mapping of TGF-β-induced phosphocomplexes at a single-cell level
title_full	Image-based high-throughput mapping of TGF-β-induced phosphocomplexes at a single-cell level
title_fullStr	Image-based high-throughput mapping of TGF-β-induced phosphocomplexes at a single-cell level
title_full_unstemmed	Image-based high-throughput mapping of TGF-β-induced phosphocomplexes at a single-cell level
title_sort	image-based high-throughput mapping of tgf-β-induced phosphocomplexes at a single-cell level
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e5ecdbaa06634e1d97b74de9458d189e
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Image-based high-throughput mapping of TGF-β-induced phosphocomplexes at a single-cell level

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