Image-based high-throughput mapping of TGF-β-induced phosphocomplexes at a single-cell level
To improve our ability to monitor cellular responses to e.g. cytokines or drugs, Lönn et al have developed a semi-automated system for large-scale in situ proximity ligation assays (isPLA) in HaCAT keratinocyte cells. Their approach expands the scope for image-based single cell analyses by combining...
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Main Authors: | Peter Lönn, Rasel A. Al-Amin, Ehsan Manouchehri Doulabi, Johan Heldin, Radiosa Gallini, Johan Björkesten, Johan Oelrich, Masood Kamali-Moghaddam, Ulf Landegren |
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Format: | article |
Language: | EN |
Published: |
Nature Portfolio
2021
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Subjects: | |
Online Access: | https://doaj.org/article/e5ecdbaa06634e1d97b74de9458d189e |
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