Identification and genetic diversity analysis of a male-sterile gene (MS1) in Japanese cedar (Cryptomeria japonica D. Don)

Abstract Identifying causative genes for a target trait in conifer reproduction is challenging for species lacking whole-genome sequences. In this study, we searched for the male-sterility gene (MS1) in Cryptomeria japonica, aiming to promote marker-assisted selection (MAS) of male-sterile C. japoni...

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Autores principales:	Yoichi Hasegawa, Saneyoshi Ueno, Fu-Jin Wei, Asako Matsumoto, Kentaro Uchiyama, Tokuko Ujino-Ihara, Tetsuji Hakamata, Takeshi Fujino, Masahiro Kasahara, Takahiro Bino, Katsushi Yamaguchi, Shuji Shigenobu, Yoshihiko Tsumura, Yoshinari Moriguchi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e5f39a8bd45048f18c38efd38044c71d
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spelling	oai:doaj.org-article:e5f39a8bd45048f18c38efd38044c71d2021-12-02T14:12:10ZIdentification and genetic diversity analysis of a male-sterile gene (MS1) in Japanese cedar (Cryptomeria japonica D. Don)10.1038/s41598-020-80688-12045-2322https://doaj.org/article/e5f39a8bd45048f18c38efd38044c71d2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80688-1https://doaj.org/toc/2045-2322Abstract Identifying causative genes for a target trait in conifer reproduction is challenging for species lacking whole-genome sequences. In this study, we searched for the male-sterility gene (MS1) in Cryptomeria japonica, aiming to promote marker-assisted selection (MAS) of male-sterile C. japonica to reduce the pollinosis caused by pollen dispersal from artificial C. japonica forests in Japan. We searched for mRNA sequences expressed in male strobili and found the gene CJt020762, coding for a lipid transfer protein containing a 4-bp deletion specific to male-sterile individuals. We also found a 30-bp deletion by sequencing the entire gene of another individual with the ms1. All nine breeding materials with the allele ms1 had either a 4-bp or 30-bp deletion in gene CJt020762, both of which are expected to result in faulty gene transcription and function. Furthermore, the 30-bp deletion was detected from three of five individuals in the Ishinomaki natural forest. From our findings, CJt020762 was considered to be the causative gene of MS1. Thus, by performing MAS using two deletion mutations as a DNA marker, it will be possible to find novel breeding materials of C. japonica with the allele ms1 adapted to the unique environment of each region of the Japanese archipelago.Yoichi HasegawaSaneyoshi UenoFu-Jin WeiAsako MatsumotoKentaro UchiyamaTokuko Ujino-IharaTetsuji HakamataTakeshi FujinoMasahiro KasaharaTakahiro BinoKatsushi YamaguchiShuji ShigenobuYoshihiko TsumuraYoshinari MoriguchiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yoichi Hasegawa Saneyoshi Ueno Fu-Jin Wei Asako Matsumoto Kentaro Uchiyama Tokuko Ujino-Ihara Tetsuji Hakamata Takeshi Fujino Masahiro Kasahara Takahiro Bino Katsushi Yamaguchi Shuji Shigenobu Yoshihiko Tsumura Yoshinari Moriguchi Identification and genetic diversity analysis of a male-sterile gene (MS1) in Japanese cedar (Cryptomeria japonica D. Don)
description	Abstract Identifying causative genes for a target trait in conifer reproduction is challenging for species lacking whole-genome sequences. In this study, we searched for the male-sterility gene (MS1) in Cryptomeria japonica, aiming to promote marker-assisted selection (MAS) of male-sterile C. japonica to reduce the pollinosis caused by pollen dispersal from artificial C. japonica forests in Japan. We searched for mRNA sequences expressed in male strobili and found the gene CJt020762, coding for a lipid transfer protein containing a 4-bp deletion specific to male-sterile individuals. We also found a 30-bp deletion by sequencing the entire gene of another individual with the ms1. All nine breeding materials with the allele ms1 had either a 4-bp or 30-bp deletion in gene CJt020762, both of which are expected to result in faulty gene transcription and function. Furthermore, the 30-bp deletion was detected from three of five individuals in the Ishinomaki natural forest. From our findings, CJt020762 was considered to be the causative gene of MS1. Thus, by performing MAS using two deletion mutations as a DNA marker, it will be possible to find novel breeding materials of C. japonica with the allele ms1 adapted to the unique environment of each region of the Japanese archipelago.
format	article
author	Yoichi Hasegawa Saneyoshi Ueno Fu-Jin Wei Asako Matsumoto Kentaro Uchiyama Tokuko Ujino-Ihara Tetsuji Hakamata Takeshi Fujino Masahiro Kasahara Takahiro Bino Katsushi Yamaguchi Shuji Shigenobu Yoshihiko Tsumura Yoshinari Moriguchi
author_facet	Yoichi Hasegawa Saneyoshi Ueno Fu-Jin Wei Asako Matsumoto Kentaro Uchiyama Tokuko Ujino-Ihara Tetsuji Hakamata Takeshi Fujino Masahiro Kasahara Takahiro Bino Katsushi Yamaguchi Shuji Shigenobu Yoshihiko Tsumura Yoshinari Moriguchi
author_sort	Yoichi Hasegawa
title	Identification and genetic diversity analysis of a male-sterile gene (MS1) in Japanese cedar (Cryptomeria japonica D. Don)
title_short	Identification and genetic diversity analysis of a male-sterile gene (MS1) in Japanese cedar (Cryptomeria japonica D. Don)
title_full	Identification and genetic diversity analysis of a male-sterile gene (MS1) in Japanese cedar (Cryptomeria japonica D. Don)
title_fullStr	Identification and genetic diversity analysis of a male-sterile gene (MS1) in Japanese cedar (Cryptomeria japonica D. Don)
title_full_unstemmed	Identification and genetic diversity analysis of a male-sterile gene (MS1) in Japanese cedar (Cryptomeria japonica D. Don)
title_sort	identification and genetic diversity analysis of a male-sterile gene (ms1) in japanese cedar (cryptomeria japonica d. don)
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e5f39a8bd45048f18c38efd38044c71d
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Identification and genetic diversity analysis of a male-sterile gene (MS1) in Japanese cedar (Cryptomeria japonica D. Don)

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