Assessment of histological characteristics, imaging markers, and rt-PA susceptibility of ex vivo venous thrombi

Abstract Venous thromboembolism is a significant source of morbidity and mortality worldwide. Catheter-directed thrombolytics is the primary treatment used to relieve critical obstructions, though its efficacy varies based on the thrombus composition. Non-responsive portions of the specimen often re...

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Autores principales:	Samuel A. Hendley, Alexey Dimov, Aarushi Bhargava, Erin Snoddy, Daniel Mansour, Rana O. Afifi, Geoffrey D. Wool, Yuanyuan Zha, Steffen Sammet, Zheng Feng Lu, Osman Ahmed, Jonathan D. Paul, Kenneth B. Bader
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e5f990347bec4bce8ca156ca2a07b3d5
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spelling	oai:doaj.org-article:e5f990347bec4bce8ca156ca2a07b3d52021-11-28T12:20:51ZAssessment of histological characteristics, imaging markers, and rt-PA susceptibility of ex vivo venous thrombi10.1038/s41598-021-02030-72045-2322https://doaj.org/article/e5f990347bec4bce8ca156ca2a07b3d52021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02030-7https://doaj.org/toc/2045-2322Abstract Venous thromboembolism is a significant source of morbidity and mortality worldwide. Catheter-directed thrombolytics is the primary treatment used to relieve critical obstructions, though its efficacy varies based on the thrombus composition. Non-responsive portions of the specimen often remain in situ, which prohibits mechanistic investigation of lytic resistance or the development of diagnostic indicators for treatment outcomes. In this study, thrombus samples extracted from venous thromboembolism patients were analyzed ex vivo to determine their histological properties, susceptibility to lytic therapy, and imaging characteristics. A wide range of thrombus morphologies were observed, with a dependence on age and etymology of the specimen. Fibrinolytic inhibitors including PAI-1, alpha 2-antiplasmin, and TAFI were present in samples, which may contribute to the response venous thrombi to catheter-directed thrombolytics. Finally, a weak but significant correlation was observed between the response of the sample to lytic drug and its magnetic microstructure assessed with a quantitative MRI sequence. These findings highlight the myriad of changes in venous thrombi that may promote lytic resistance, and imaging metrics that correlate with treatment outcomes.Samuel A. HendleyAlexey DimovAarushi BhargavaErin SnoddyDaniel MansourRana O. AfifiGeoffrey D. WoolYuanyuan ZhaSteffen SammetZheng Feng LuOsman AhmedJonathan D. PaulKenneth B. BaderNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Samuel A. Hendley Alexey Dimov Aarushi Bhargava Erin Snoddy Daniel Mansour Rana O. Afifi Geoffrey D. Wool Yuanyuan Zha Steffen Sammet Zheng Feng Lu Osman Ahmed Jonathan D. Paul Kenneth B. Bader Assessment of histological characteristics, imaging markers, and rt-PA susceptibility of ex vivo venous thrombi
description	Abstract Venous thromboembolism is a significant source of morbidity and mortality worldwide. Catheter-directed thrombolytics is the primary treatment used to relieve critical obstructions, though its efficacy varies based on the thrombus composition. Non-responsive portions of the specimen often remain in situ, which prohibits mechanistic investigation of lytic resistance or the development of diagnostic indicators for treatment outcomes. In this study, thrombus samples extracted from venous thromboembolism patients were analyzed ex vivo to determine their histological properties, susceptibility to lytic therapy, and imaging characteristics. A wide range of thrombus morphologies were observed, with a dependence on age and etymology of the specimen. Fibrinolytic inhibitors including PAI-1, alpha 2-antiplasmin, and TAFI were present in samples, which may contribute to the response venous thrombi to catheter-directed thrombolytics. Finally, a weak but significant correlation was observed between the response of the sample to lytic drug and its magnetic microstructure assessed with a quantitative MRI sequence. These findings highlight the myriad of changes in venous thrombi that may promote lytic resistance, and imaging metrics that correlate with treatment outcomes.
format	article
author	Samuel A. Hendley Alexey Dimov Aarushi Bhargava Erin Snoddy Daniel Mansour Rana O. Afifi Geoffrey D. Wool Yuanyuan Zha Steffen Sammet Zheng Feng Lu Osman Ahmed Jonathan D. Paul Kenneth B. Bader
author_facet	Samuel A. Hendley Alexey Dimov Aarushi Bhargava Erin Snoddy Daniel Mansour Rana O. Afifi Geoffrey D. Wool Yuanyuan Zha Steffen Sammet Zheng Feng Lu Osman Ahmed Jonathan D. Paul Kenneth B. Bader
author_sort	Samuel A. Hendley
title	Assessment of histological characteristics, imaging markers, and rt-PA susceptibility of ex vivo venous thrombi
title_short	Assessment of histological characteristics, imaging markers, and rt-PA susceptibility of ex vivo venous thrombi
title_full	Assessment of histological characteristics, imaging markers, and rt-PA susceptibility of ex vivo venous thrombi
title_fullStr	Assessment of histological characteristics, imaging markers, and rt-PA susceptibility of ex vivo venous thrombi
title_full_unstemmed	Assessment of histological characteristics, imaging markers, and rt-PA susceptibility of ex vivo venous thrombi
title_sort	assessment of histological characteristics, imaging markers, and rt-pa susceptibility of ex vivo venous thrombi
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e5f990347bec4bce8ca156ca2a07b3d5
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Assessment of histological characteristics, imaging markers, and rt-PA susceptibility of ex vivo venous thrombi

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