Fourier transform infrared imaging and infrared fiber optic probe spectroscopy identify collagen type in connective tissues.

Hyaline cartilage and mechanically inferior fibrocartilage consisting of mixed collagen types are frequently found together in repairing articular cartilage. The present study seeks to develop methodology to identify collagen type and other tissue components using Fourier transform infrared (FTIR) s...

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Autores principales:	Arash Hanifi, Helen McCarthy, Sally Roberts, Nancy Pleshko
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e5ff2d4e7d3f4059b44b324b132da8d8
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spelling	oai:doaj.org-article:e5ff2d4e7d3f4059b44b324b132da8d82021-11-18T07:44:30ZFourier transform infrared imaging and infrared fiber optic probe spectroscopy identify collagen type in connective tissues.1932-620310.1371/journal.pone.0064822https://doaj.org/article/e5ff2d4e7d3f4059b44b324b132da8d82013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23717662/?tool=EBIhttps://doaj.org/toc/1932-6203Hyaline cartilage and mechanically inferior fibrocartilage consisting of mixed collagen types are frequently found together in repairing articular cartilage. The present study seeks to develop methodology to identify collagen type and other tissue components using Fourier transform infrared (FTIR) spectral evaluation of matrix composition in combination with multivariate analyses. FTIR spectra of the primary molecular components of repair cartilage, types I and II collagen, and aggrecan, were used to develop multivariate spectral models for discrimination of the matrix components of the tissues of interest. Infrared imaging data were collected from bovine bone, tendon, normal cartilage, meniscus and human repair cartilage tissues, and composition predicted using partial least squares analyses. Histology and immunohistochemistry results were used as standards for validation. Infrared fiber optic probe spectral data were also obtained from meniscus (a tissue with mixed collagen types) to evaluate the potential of this method for identification of collagen type in a minimally-invasive clinical application. Concentration profiles of the tissue components obtained from multivariate analysis were in excellent agreement with histology and immunohistochemistry results. Bone and tendon showed a uniform distribution of predominantly type I collagen through the tissue. Normal cartilage showed a distribution of type II collagen and proteoglycan similar to the known composition, while in repair cartilage, the spectral distribution of both types I and II collagen were similar to that observed via immunohistochemistry. Using the probe, the outer and inner regions of the meniscus were shown to be primarily composed of type I and II collagen, respectively, in accordance with immunohistochemistry data. In summary, multivariate analysis of infrared spectra can indeed be used to differentiate collagen type I and type II, even in the presence of proteoglycan, in connective tissues, using both imaging and fiber optic methodology. This has great potential for clinical in situ applications for monitoring tissue repair.Arash HanifiHelen McCarthySally RobertsNancy PleshkoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e64822 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Arash Hanifi Helen McCarthy Sally Roberts Nancy Pleshko Fourier transform infrared imaging and infrared fiber optic probe spectroscopy identify collagen type in connective tissues.
description	Hyaline cartilage and mechanically inferior fibrocartilage consisting of mixed collagen types are frequently found together in repairing articular cartilage. The present study seeks to develop methodology to identify collagen type and other tissue components using Fourier transform infrared (FTIR) spectral evaluation of matrix composition in combination with multivariate analyses. FTIR spectra of the primary molecular components of repair cartilage, types I and II collagen, and aggrecan, were used to develop multivariate spectral models for discrimination of the matrix components of the tissues of interest. Infrared imaging data were collected from bovine bone, tendon, normal cartilage, meniscus and human repair cartilage tissues, and composition predicted using partial least squares analyses. Histology and immunohistochemistry results were used as standards for validation. Infrared fiber optic probe spectral data were also obtained from meniscus (a tissue with mixed collagen types) to evaluate the potential of this method for identification of collagen type in a minimally-invasive clinical application. Concentration profiles of the tissue components obtained from multivariate analysis were in excellent agreement with histology and immunohistochemistry results. Bone and tendon showed a uniform distribution of predominantly type I collagen through the tissue. Normal cartilage showed a distribution of type II collagen and proteoglycan similar to the known composition, while in repair cartilage, the spectral distribution of both types I and II collagen were similar to that observed via immunohistochemistry. Using the probe, the outer and inner regions of the meniscus were shown to be primarily composed of type I and II collagen, respectively, in accordance with immunohistochemistry data. In summary, multivariate analysis of infrared spectra can indeed be used to differentiate collagen type I and type II, even in the presence of proteoglycan, in connective tissues, using both imaging and fiber optic methodology. This has great potential for clinical in situ applications for monitoring tissue repair.
format	article
author	Arash Hanifi Helen McCarthy Sally Roberts Nancy Pleshko
author_facet	Arash Hanifi Helen McCarthy Sally Roberts Nancy Pleshko
author_sort	Arash Hanifi
title	Fourier transform infrared imaging and infrared fiber optic probe spectroscopy identify collagen type in connective tissues.
title_short	Fourier transform infrared imaging and infrared fiber optic probe spectroscopy identify collagen type in connective tissues.
title_full	Fourier transform infrared imaging and infrared fiber optic probe spectroscopy identify collagen type in connective tissues.
title_fullStr	Fourier transform infrared imaging and infrared fiber optic probe spectroscopy identify collagen type in connective tissues.
title_full_unstemmed	Fourier transform infrared imaging and infrared fiber optic probe spectroscopy identify collagen type in connective tissues.
title_sort	fourier transform infrared imaging and infrared fiber optic probe spectroscopy identify collagen type in connective tissues.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/e5ff2d4e7d3f4059b44b324b132da8d8
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Fourier transform infrared imaging and infrared fiber optic probe spectroscopy identify collagen type in connective tissues.

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