CHARACTERIZATION OF HEAT SHOCK PROTEINS IN BRUCELLA ABORTUS AND INTERACTION OF THESE PROTEINS WITH PATIENT AND CONTROL SERA

BACKGROUND AND OBJECTIVE: The bacteria begin to synthesize heat shock proteins (hsp) when facing elevated temperatures. Some of the hsps recognized during infection by host immune system. The goal of this study was to determine the synthetic pattern of hsps in brucella abortus that were subjected to...

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Autores principales:	R Rajabnia, N Amirmozaffari, F Ghazi, A Mostafazadeh, A Mostafaie
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2008
Materias:	brucella abortus heat shock proteins brucellosis Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/e616db5020494e9a8087b7381d896711
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spelling	oai:doaj.org-article:e616db5020494e9a8087b7381d8967112021-11-10T09:07:10ZCHARACTERIZATION OF HEAT SHOCK PROTEINS IN BRUCELLA ABORTUS AND INTERACTION OF THESE PROTEINS WITH PATIENT AND CONTROL SERA1561-41072251-7170https://doaj.org/article/e616db5020494e9a8087b7381d8967112008-01-01T00:00:00Zhttp://jbums.org/article-1-2489-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: The bacteria begin to synthesize heat shock proteins (hsp) when facing elevated temperatures. Some of the hsps recognized during infection by host immune system. The goal of this study was to determine the synthetic pattern of hsps in brucella abortus that were subjected to different heat shocks and antigenecity of hsps in both patient and healthy sera by immuno blot assay.METHODS: In this experimental trial study, five brucella abortus were isolated from cows and were subjected to 39, 40, and 42ºC heat shocks. Then cell proteins were extracted and electrophoresis in polyacrylamide gels (SDS-PAGE) was performed. Finally antigenecity of hsps (hsp60) in healthy as well as patient sera were surveyed by immuno blot.FINDINGS: The major protein groups were in the range of 30-75 and 14-20 KDa in SDS-PAGE. The amounts of a 60 KDa protein band (hsp60) was significantly enhanced following heat shock as compared to the unheated cells. The sera from brucella patients reacted with several of these cell derived protein bands in immuno blots, none of which were reactive with sera from healthy individuals. The 60 KDa band was the most significant and showed strong reactions with all brucellosis sera samples. CONCLUSION: This study showed the brucella abortus produces heat shock proteins, especially hsp60, in response to heat shock. Brucellosis patient’s sera reacted strongly with these molecules. This observation points to the antigenecity properties of hsps, specially the overwhelming response to hsp-60. Therefore, probably the hsp60 can be an antigen candidate for ELISA test development as well as for engineering sub unit vaccine against brucella.R Rajabnia,N Amirmozaffari,F Ghazi,A MostafazadehA MostafaieBabol University of Medical Sciencesarticlebrucella abortusheat shock proteinsbrucellosisMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 9, Iss 6, Pp 22-28 (2008)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	brucella abortus heat shock proteins brucellosis Medicine R Medicine (General) R5-920
spellingShingle	brucella abortus heat shock proteins brucellosis Medicine R Medicine (General) R5-920 R Rajabnia, N Amirmozaffari, F Ghazi, A Mostafazadeh A Mostafaie CHARACTERIZATION OF HEAT SHOCK PROTEINS IN BRUCELLA ABORTUS AND INTERACTION OF THESE PROTEINS WITH PATIENT AND CONTROL SERA
description	BACKGROUND AND OBJECTIVE: The bacteria begin to synthesize heat shock proteins (hsp) when facing elevated temperatures. Some of the hsps recognized during infection by host immune system. The goal of this study was to determine the synthetic pattern of hsps in brucella abortus that were subjected to different heat shocks and antigenecity of hsps in both patient and healthy sera by immuno blot assay.METHODS: In this experimental trial study, five brucella abortus were isolated from cows and were subjected to 39, 40, and 42ºC heat shocks. Then cell proteins were extracted and electrophoresis in polyacrylamide gels (SDS-PAGE) was performed. Finally antigenecity of hsps (hsp60) in healthy as well as patient sera were surveyed by immuno blot.FINDINGS: The major protein groups were in the range of 30-75 and 14-20 KDa in SDS-PAGE. The amounts of a 60 KDa protein band (hsp60) was significantly enhanced following heat shock as compared to the unheated cells. The sera from brucella patients reacted with several of these cell derived protein bands in immuno blots, none of which were reactive with sera from healthy individuals. The 60 KDa band was the most significant and showed strong reactions with all brucellosis sera samples. CONCLUSION: This study showed the brucella abortus produces heat shock proteins, especially hsp60, in response to heat shock. Brucellosis patient’s sera reacted strongly with these molecules. This observation points to the antigenecity properties of hsps, specially the overwhelming response to hsp-60. Therefore, probably the hsp60 can be an antigen candidate for ELISA test development as well as for engineering sub unit vaccine against brucella.
format	article
author	R Rajabnia, N Amirmozaffari, F Ghazi, A Mostafazadeh A Mostafaie
author_facet	R Rajabnia, N Amirmozaffari, F Ghazi, A Mostafazadeh A Mostafaie
author_sort	R Rajabnia,
title	CHARACTERIZATION OF HEAT SHOCK PROTEINS IN BRUCELLA ABORTUS AND INTERACTION OF THESE PROTEINS WITH PATIENT AND CONTROL SERA
title_short	CHARACTERIZATION OF HEAT SHOCK PROTEINS IN BRUCELLA ABORTUS AND INTERACTION OF THESE PROTEINS WITH PATIENT AND CONTROL SERA
title_full	CHARACTERIZATION OF HEAT SHOCK PROTEINS IN BRUCELLA ABORTUS AND INTERACTION OF THESE PROTEINS WITH PATIENT AND CONTROL SERA
title_fullStr	CHARACTERIZATION OF HEAT SHOCK PROTEINS IN BRUCELLA ABORTUS AND INTERACTION OF THESE PROTEINS WITH PATIENT AND CONTROL SERA
title_full_unstemmed	CHARACTERIZATION OF HEAT SHOCK PROTEINS IN BRUCELLA ABORTUS AND INTERACTION OF THESE PROTEINS WITH PATIENT AND CONTROL SERA
title_sort	characterization of heat shock proteins in brucella abortus and interaction of these proteins with patient and control sera
publisher	Babol University of Medical Sciences
publishDate	2008
url	https://doaj.org/article/e616db5020494e9a8087b7381d896711
work_keys_str_mv	AT rrajabnia characterizationofheatshockproteinsinbrucellaabortusandinteractionoftheseproteinswithpatientandcontrolsera AT namirmozaffari characterizationofheatshockproteinsinbrucellaabortusandinteractionoftheseproteinswithpatientandcontrolsera AT fghazi characterizationofheatshockproteinsinbrucellaabortusandinteractionoftheseproteinswithpatientandcontrolsera AT amostafazadeh characterizationofheatshockproteinsinbrucellaabortusandinteractionoftheseproteinswithpatientandcontrolsera AT amostafaie characterizationofheatshockproteinsinbrucellaabortusandinteractionoftheseproteinswithpatientandcontrolsera
_version_	1718440336185884672

CHARACTERIZATION OF HEAT SHOCK PROTEINS IN BRUCELLA ABORTUS AND INTERACTION OF THESE PROTEINS WITH PATIENT AND CONTROL SERA

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