Potassium enhances cadmium resistance ability of Panax notoginseng by brassinolide signaling pathway-regulated cell wall pectin metabolism

The mechanism of how potassium (K) attenuates cadmium (Cd)-induced demethylation and anabolism of cell wall (CW) pectin through the brassinolide (BR) signaling pathway was verified in Panax notoginseng (Burk.). The P. notoginseng pectin methylesterase gene (PnPME1) was cloned and functionally verifi...

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Autores principales:	Pengfei Liu, Zhengqiang Jin, Chunyan Dai, Lanping Guo, Xiuming Cui, Ye Yang
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Heavy metal Abiotic stress Sanqi PnPME1 Traditional Chinese medicine Environmental pollution TD172-193.5 Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/e61fb3581c784c21bb1ad8bfaa959296
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spelling	oai:doaj.org-article:e61fb3581c784c21bb1ad8bfaa9592962021-11-06T04:16:31ZPotassium enhances cadmium resistance ability of Panax notoginseng by brassinolide signaling pathway-regulated cell wall pectin metabolism0147-651310.1016/j.ecoenv.2021.112906https://doaj.org/article/e61fb3581c784c21bb1ad8bfaa9592962021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0147651321010186https://doaj.org/toc/0147-6513The mechanism of how potassium (K) attenuates cadmium (Cd)-induced demethylation and anabolism of cell wall (CW) pectin through the brassinolide (BR) signaling pathway was verified in Panax notoginseng (Burk.). The P. notoginseng pectin methylesterase gene (PnPME1) was cloned and functionally verified in tobacco. Pectin and BR metabolism, Cd content and the pectin methylation degree (PMD) were detected in response to K, 2,4-epibrassinolide (EBL), and brassinazole treatments of P. notoginseng and tobacco under Cd stress. Activity of the main root pectin methylesterase enzyme (PME) was promoted by 22.29% under the EBL treatment, and Cd content increased by 29.03% under Cd stress. Potassium reduced PME activity and Cd content in main root pectin by 61.03% and 50.73%, respectively, under the EBL and Cd co-treatment. Potassium inhibited the promoting effects of Cd stress on the expression of PnPME1 by 57.04%. Potassium also inhibited expression of BR synthesis genes PnDET2, PnROT3, PnCYP90A1, and PnBR6OX1 by 65.61%, 52.02%, 47.36%, and 55.16%, respectively, and reduced the accumulation of Cd. The PnPME1 was located in the CW. The activity of transgenic tobacco root PME was higher than that of the wild-type, while the PMD was significantly lower. The regulatory effects of K and EBL on tobacco root pectin metabolism were consistent with those in P. notoginseng. In conclusion, K downregulated the expression of BR synthesis genes in P. notoginseng roots under Cd stress and reduced the production of BRs, which inhibited PnPME1 expression. The reduction in PME activity increased the PMD, which reduced the accumulation of Cd.Pengfei LiuZhengqiang JinChunyan DaiLanping GuoXiuming CuiYe YangElsevierarticleHeavy metalAbiotic stressSanqiPnPME1Traditional Chinese medicineEnvironmental pollutionTD172-193.5Environmental sciencesGE1-350ENEcotoxicology and Environmental Safety, Vol 227, Iss , Pp 112906- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Heavy metal Abiotic stress Sanqi PnPME1 Traditional Chinese medicine Environmental pollution TD172-193.5 Environmental sciences GE1-350
spellingShingle	Heavy metal Abiotic stress Sanqi PnPME1 Traditional Chinese medicine Environmental pollution TD172-193.5 Environmental sciences GE1-350 Pengfei Liu Zhengqiang Jin Chunyan Dai Lanping Guo Xiuming Cui Ye Yang Potassium enhances cadmium resistance ability of Panax notoginseng by brassinolide signaling pathway-regulated cell wall pectin metabolism
description	The mechanism of how potassium (K) attenuates cadmium (Cd)-induced demethylation and anabolism of cell wall (CW) pectin through the brassinolide (BR) signaling pathway was verified in Panax notoginseng (Burk.). The P. notoginseng pectin methylesterase gene (PnPME1) was cloned and functionally verified in tobacco. Pectin and BR metabolism, Cd content and the pectin methylation degree (PMD) were detected in response to K, 2,4-epibrassinolide (EBL), and brassinazole treatments of P. notoginseng and tobacco under Cd stress. Activity of the main root pectin methylesterase enzyme (PME) was promoted by 22.29% under the EBL treatment, and Cd content increased by 29.03% under Cd stress. Potassium reduced PME activity and Cd content in main root pectin by 61.03% and 50.73%, respectively, under the EBL and Cd co-treatment. Potassium inhibited the promoting effects of Cd stress on the expression of PnPME1 by 57.04%. Potassium also inhibited expression of BR synthesis genes PnDET2, PnROT3, PnCYP90A1, and PnBR6OX1 by 65.61%, 52.02%, 47.36%, and 55.16%, respectively, and reduced the accumulation of Cd. The PnPME1 was located in the CW. The activity of transgenic tobacco root PME was higher than that of the wild-type, while the PMD was significantly lower. The regulatory effects of K and EBL on tobacco root pectin metabolism were consistent with those in P. notoginseng. In conclusion, K downregulated the expression of BR synthesis genes in P. notoginseng roots under Cd stress and reduced the production of BRs, which inhibited PnPME1 expression. The reduction in PME activity increased the PMD, which reduced the accumulation of Cd.
format	article
author	Pengfei Liu Zhengqiang Jin Chunyan Dai Lanping Guo Xiuming Cui Ye Yang
author_facet	Pengfei Liu Zhengqiang Jin Chunyan Dai Lanping Guo Xiuming Cui Ye Yang
author_sort	Pengfei Liu
title	Potassium enhances cadmium resistance ability of Panax notoginseng by brassinolide signaling pathway-regulated cell wall pectin metabolism
title_short	Potassium enhances cadmium resistance ability of Panax notoginseng by brassinolide signaling pathway-regulated cell wall pectin metabolism
title_full	Potassium enhances cadmium resistance ability of Panax notoginseng by brassinolide signaling pathway-regulated cell wall pectin metabolism
title_fullStr	Potassium enhances cadmium resistance ability of Panax notoginseng by brassinolide signaling pathway-regulated cell wall pectin metabolism
title_full_unstemmed	Potassium enhances cadmium resistance ability of Panax notoginseng by brassinolide signaling pathway-regulated cell wall pectin metabolism
title_sort	potassium enhances cadmium resistance ability of panax notoginseng by brassinolide signaling pathway-regulated cell wall pectin metabolism
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/e61fb3581c784c21bb1ad8bfaa959296
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Potassium enhances cadmium resistance ability of Panax notoginseng by brassinolide signaling pathway-regulated cell wall pectin metabolism

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