Transformation and Overexpression of Primary Cell Wall Synthesis-Related Zinc Finger Gene Gh_A07G1537 to Improve Fiber Length in Cotton

Molecular interventions have helped to explore the genes involved in fiber length, fiber strength, and other quality parameters with improved characteristics, particularly in cotton. The current study is an extension and functional validation of previous findings that Gh_A07G1537 influences fiber le...

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Autores principales:	Abdul Razzaq, Muhammad Mubashar Zafar, Pengtao Li, Ge Qun, Xiaoying Deng, Arfan Ali, Abdul Hafeez, Muhammad Irfan, Aiying Liu, Maozhi Ren, Haihong Shang, Yuzhen Shi, Wankui Gong, Youlu Yuan
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	CSSLs fiber length zinc finger genetic transformation cotton biology Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/e61fc8e980fa4f0191583289e414ccbe
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spelling	oai:doaj.org-article:e61fc8e980fa4f0191583289e414ccbe2021-11-05T16:52:29ZTransformation and Overexpression of Primary Cell Wall Synthesis-Related Zinc Finger Gene Gh_A07G1537 to Improve Fiber Length in Cotton1664-462X10.3389/fpls.2021.777794https://doaj.org/article/e61fc8e980fa4f0191583289e414ccbe2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.777794/fullhttps://doaj.org/toc/1664-462XMolecular interventions have helped to explore the genes involved in fiber length, fiber strength, and other quality parameters with improved characteristics, particularly in cotton. The current study is an extension and functional validation of previous findings that Gh_A07G1537 influences fiber length in cotton using a chromosomal segment substitution line MBI7747 through RNA-seq data. The recombinant Gh_A07G1537 derived from the MBI7747 line was over-expressed in CCRI24, a genotype with a low profile of fiber quality parameters. Putative transformants were selected on MS medium containing hygromycin (25mg/ml), acclimatized, and shifted to a greenhouse for further growth and proliferation. Transgene integration was validated through PCR and Southern Blot analysis. Stable integration of the transgene (ΔGh_A07G1537) was validated by tracking its expression in different generations (T0, T1, and T2) of transformed cotton plants. It was found to be 2.97-, 2.86-, and 2.92-folds higher expression in T0, T1, and T2 plants, respectively, of transgenic compared with non-transgenic cotton plants. Fiber quality parameters were also observed to be improved in the engineered cotton line. Genetic modifications of Gh_A07G1537 support the improvement in fiber quality parameters and should be appreciated for the textile industry.Abdul RazzaqAbdul RazzaqMuhammad Mubashar ZafarPengtao LiGe QunXiaoying DengArfan AliAbdul HafeezMuhammad IrfanAiying LiuMaozhi RenHaihong ShangHaihong ShangYuzhen ShiWankui GongYoulu YuanYoulu YuanFrontiers Media S.A.articleCSSLsfiber lengthzinc fingergenetic transformationcotton biologyPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	CSSLs fiber length zinc finger genetic transformation cotton biology Plant culture SB1-1110
spellingShingle	CSSLs fiber length zinc finger genetic transformation cotton biology Plant culture SB1-1110 Abdul Razzaq Abdul Razzaq Muhammad Mubashar Zafar Pengtao Li Ge Qun Xiaoying Deng Arfan Ali Abdul Hafeez Muhammad Irfan Aiying Liu Maozhi Ren Haihong Shang Haihong Shang Yuzhen Shi Wankui Gong Youlu Yuan Youlu Yuan Transformation and Overexpression of Primary Cell Wall Synthesis-Related Zinc Finger Gene Gh_A07G1537 to Improve Fiber Length in Cotton
description	Molecular interventions have helped to explore the genes involved in fiber length, fiber strength, and other quality parameters with improved characteristics, particularly in cotton. The current study is an extension and functional validation of previous findings that Gh_A07G1537 influences fiber length in cotton using a chromosomal segment substitution line MBI7747 through RNA-seq data. The recombinant Gh_A07G1537 derived from the MBI7747 line was over-expressed in CCRI24, a genotype with a low profile of fiber quality parameters. Putative transformants were selected on MS medium containing hygromycin (25mg/ml), acclimatized, and shifted to a greenhouse for further growth and proliferation. Transgene integration was validated through PCR and Southern Blot analysis. Stable integration of the transgene (ΔGh_A07G1537) was validated by tracking its expression in different generations (T0, T1, and T2) of transformed cotton plants. It was found to be 2.97-, 2.86-, and 2.92-folds higher expression in T0, T1, and T2 plants, respectively, of transgenic compared with non-transgenic cotton plants. Fiber quality parameters were also observed to be improved in the engineered cotton line. Genetic modifications of Gh_A07G1537 support the improvement in fiber quality parameters and should be appreciated for the textile industry.
format	article
author	Abdul Razzaq Abdul Razzaq Muhammad Mubashar Zafar Pengtao Li Ge Qun Xiaoying Deng Arfan Ali Abdul Hafeez Muhammad Irfan Aiying Liu Maozhi Ren Haihong Shang Haihong Shang Yuzhen Shi Wankui Gong Youlu Yuan Youlu Yuan
author_facet	Abdul Razzaq Abdul Razzaq Muhammad Mubashar Zafar Pengtao Li Ge Qun Xiaoying Deng Arfan Ali Abdul Hafeez Muhammad Irfan Aiying Liu Maozhi Ren Haihong Shang Haihong Shang Yuzhen Shi Wankui Gong Youlu Yuan Youlu Yuan
author_sort	Abdul Razzaq
title	Transformation and Overexpression of Primary Cell Wall Synthesis-Related Zinc Finger Gene Gh_A07G1537 to Improve Fiber Length in Cotton
title_short	Transformation and Overexpression of Primary Cell Wall Synthesis-Related Zinc Finger Gene Gh_A07G1537 to Improve Fiber Length in Cotton
title_full	Transformation and Overexpression of Primary Cell Wall Synthesis-Related Zinc Finger Gene Gh_A07G1537 to Improve Fiber Length in Cotton
title_fullStr	Transformation and Overexpression of Primary Cell Wall Synthesis-Related Zinc Finger Gene Gh_A07G1537 to Improve Fiber Length in Cotton
title_full_unstemmed	Transformation and Overexpression of Primary Cell Wall Synthesis-Related Zinc Finger Gene Gh_A07G1537 to Improve Fiber Length in Cotton
title_sort	transformation and overexpression of primary cell wall synthesis-related zinc finger gene gh_a07g1537 to improve fiber length in cotton
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/e61fc8e980fa4f0191583289e414ccbe
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Transformation and Overexpression of Primary Cell Wall Synthesis-Related Zinc Finger Gene Gh_A07G1537 to Improve Fiber Length in Cotton

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