Characterization of hair follicle development in engineered skin substitutes.

Generation of skin appendages in engineered skin substitutes has been limited by lack of trichogenic potency in cultured postnatal cells. To investigate the feasibility and the limitation of hair regeneration, engineered skin substitutes were prepared with chimeric populations of cultured human kera...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Penkanok Sriwiriyanont, Kaari A Lynch, Kevin L McFarland, Dorothy M Supp, Steven T Boyce
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e62437f2037748f89138c08cb03c87cf
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e62437f2037748f89138c08cb03c87cf
record_format	dspace
spelling	oai:doaj.org-article:e62437f2037748f89138c08cb03c87cf2021-11-18T07:41:34ZCharacterization of hair follicle development in engineered skin substitutes.1932-620310.1371/journal.pone.0065664https://doaj.org/article/e62437f2037748f89138c08cb03c87cf2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23799033/?tool=EBIhttps://doaj.org/toc/1932-6203Generation of skin appendages in engineered skin substitutes has been limited by lack of trichogenic potency in cultured postnatal cells. To investigate the feasibility and the limitation of hair regeneration, engineered skin substitutes were prepared with chimeric populations of cultured human keratinocytes from neonatal foreskins and cultured murine dermal papilla cells from adult GFP transgenic mice and grafted orthotopically to full-thickness wounds on athymic mice. Non-cultured dissociated neonatal murine-only skin cells, or cultured human-only skin keratinocytes and fibroblasts without dermal papilla cells served as positive and negative controls respectively. In this study, neonatal murine-only skin substitutes formed external hairs and sebaceous glands, chimeric skin substitutes formed pigmented hairs without sebaceous glands, and human-only skin substitutes formed no follicles or glands. Although chimeric hair cannot erupt readily, removal of upper skin layer exposed keratinized hair shafts at the skin surface. Development of incomplete pilosebaceous units in chimeric hair corresponded with upregulation of hair-related genes, LEF1 and WNT10B, and downregulation of a marker of sebaceous glands, Steroyl-CoA desaturase. Transepidermal water loss was normal in all conditions. This study demonstrated that while sebaceous glands may be involved in hair eruption, they are not required for hair development in engineered skin substitutes.Penkanok SriwiriyanontKaari A LynchKevin L McFarlandDorothy M SuppSteven T BoycePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 6, p e65664 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Penkanok Sriwiriyanont Kaari A Lynch Kevin L McFarland Dorothy M Supp Steven T Boyce Characterization of hair follicle development in engineered skin substitutes.
description	Generation of skin appendages in engineered skin substitutes has been limited by lack of trichogenic potency in cultured postnatal cells. To investigate the feasibility and the limitation of hair regeneration, engineered skin substitutes were prepared with chimeric populations of cultured human keratinocytes from neonatal foreskins and cultured murine dermal papilla cells from adult GFP transgenic mice and grafted orthotopically to full-thickness wounds on athymic mice. Non-cultured dissociated neonatal murine-only skin cells, or cultured human-only skin keratinocytes and fibroblasts without dermal papilla cells served as positive and negative controls respectively. In this study, neonatal murine-only skin substitutes formed external hairs and sebaceous glands, chimeric skin substitutes formed pigmented hairs without sebaceous glands, and human-only skin substitutes formed no follicles or glands. Although chimeric hair cannot erupt readily, removal of upper skin layer exposed keratinized hair shafts at the skin surface. Development of incomplete pilosebaceous units in chimeric hair corresponded with upregulation of hair-related genes, LEF1 and WNT10B, and downregulation of a marker of sebaceous glands, Steroyl-CoA desaturase. Transepidermal water loss was normal in all conditions. This study demonstrated that while sebaceous glands may be involved in hair eruption, they are not required for hair development in engineered skin substitutes.
format	article
author	Penkanok Sriwiriyanont Kaari A Lynch Kevin L McFarland Dorothy M Supp Steven T Boyce
author_facet	Penkanok Sriwiriyanont Kaari A Lynch Kevin L McFarland Dorothy M Supp Steven T Boyce
author_sort	Penkanok Sriwiriyanont
title	Characterization of hair follicle development in engineered skin substitutes.
title_short	Characterization of hair follicle development in engineered skin substitutes.
title_full	Characterization of hair follicle development in engineered skin substitutes.
title_fullStr	Characterization of hair follicle development in engineered skin substitutes.
title_full_unstemmed	Characterization of hair follicle development in engineered skin substitutes.
title_sort	characterization of hair follicle development in engineered skin substitutes.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/e62437f2037748f89138c08cb03c87cf
work_keys_str_mv	AT penkanoksriwiriyanont characterizationofhairfollicledevelopmentinengineeredskinsubstitutes AT kaarialynch characterizationofhairfollicledevelopmentinengineeredskinsubstitutes AT kevinlmcfarland characterizationofhairfollicledevelopmentinengineeredskinsubstitutes AT dorothymsupp characterizationofhairfollicledevelopmentinengineeredskinsubstitutes AT steventboyce characterizationofhairfollicledevelopmentinengineeredskinsubstitutes
_version_	1718423134126735360

Characterization of hair follicle development in engineered skin substitutes.

Ejemplares similares