Molecular Detection of Biological Agents in the Field: Then and Now

ABSTRACT Molecular detection of biological agents in the field has traditionally relied on the use of quantitative real-time PCR (qPCR), which now includes commercially available instruments that can be used in the laboratory or field. Adapting this technology for field-forward applications necessit...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kenneth B. Yeh, Hillary Wood, Matt Scullion, Joseph A. Russell, Kyle Parker, Bryan T. Gnade, Anthony R. Jones, Christopher Whittier, Kay Mereish
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	detection real-time PCR diagnostics genomic sequencing field laboratory Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/e625aaf913c14b7fb1a7a243be7d30da
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e625aaf913c14b7fb1a7a243be7d30da
record_format	dspace
spelling	oai:doaj.org-article:e625aaf913c14b7fb1a7a243be7d30da2021-11-15T15:22:24ZMolecular Detection of Biological Agents in the Field: Then and Now10.1128/mSphere.00695-192379-5042https://doaj.org/article/e625aaf913c14b7fb1a7a243be7d30da2019-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00695-19https://doaj.org/toc/2379-5042ABSTRACT Molecular detection of biological agents in the field has traditionally relied on the use of quantitative real-time PCR (qPCR), which now includes commercially available instruments that can be used in the laboratory or field. Adapting this technology for field-forward applications necessitated innovation to minimize size, weight, and power requirements. Rugged, portable instruments, efficient power sources, freeze-dried reagents, data communications, and standard operating procedures for minimally trained users are some examples of limitations that have been overcome to allow qPCR-based data to be generated at the point of need. Despite the high specificity and sensitivity of qPCR, the assays require a priori sequence-based knowledge of the etiological agent to design and produce specific targeted assays with primers and probes. However, in many cases the etiological agent may not be known and pathogen identification must rely on the use of an untargeted screening method. By extracting, preparing, and sequencing all of the genomic material in a particular sample at once, known as metagenomics, a less biased view of the biological entities in that sample can be ascertained. Using metagenomics methods in the field requires the development and optimization of straightforward sample preparation, sequencing, and bioinformatics workflows reminiscent of the challenges faced during the development of field-forward qPCR 15 years ago. To review the state of qPCR and sequencing in the field, we summarized a panel discussion from the 2019 ASM Biothreats Conference. Our discussion focused on the development, evolution, and comparison of molecular methods for biological agents and their utility in the field.Kenneth B. YehHillary WoodMatt ScullionJoseph A. RussellKyle ParkerBryan T. GnadeAnthony R. JonesChristopher WhittierKay MereishAmerican Society for Microbiologyarticledetectionreal-time PCRdiagnosticsgenomic sequencingfield laboratoryMicrobiologyQR1-502ENmSphere, Vol 4, Iss 6 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	detection real-time PCR diagnostics genomic sequencing field laboratory Microbiology QR1-502
spellingShingle	detection real-time PCR diagnostics genomic sequencing field laboratory Microbiology QR1-502 Kenneth B. Yeh Hillary Wood Matt Scullion Joseph A. Russell Kyle Parker Bryan T. Gnade Anthony R. Jones Christopher Whittier Kay Mereish Molecular Detection of Biological Agents in the Field: Then and Now
description	ABSTRACT Molecular detection of biological agents in the field has traditionally relied on the use of quantitative real-time PCR (qPCR), which now includes commercially available instruments that can be used in the laboratory or field. Adapting this technology for field-forward applications necessitated innovation to minimize size, weight, and power requirements. Rugged, portable instruments, efficient power sources, freeze-dried reagents, data communications, and standard operating procedures for minimally trained users are some examples of limitations that have been overcome to allow qPCR-based data to be generated at the point of need. Despite the high specificity and sensitivity of qPCR, the assays require a priori sequence-based knowledge of the etiological agent to design and produce specific targeted assays with primers and probes. However, in many cases the etiological agent may not be known and pathogen identification must rely on the use of an untargeted screening method. By extracting, preparing, and sequencing all of the genomic material in a particular sample at once, known as metagenomics, a less biased view of the biological entities in that sample can be ascertained. Using metagenomics methods in the field requires the development and optimization of straightforward sample preparation, sequencing, and bioinformatics workflows reminiscent of the challenges faced during the development of field-forward qPCR 15 years ago. To review the state of qPCR and sequencing in the field, we summarized a panel discussion from the 2019 ASM Biothreats Conference. Our discussion focused on the development, evolution, and comparison of molecular methods for biological agents and their utility in the field.
format	article
author	Kenneth B. Yeh Hillary Wood Matt Scullion Joseph A. Russell Kyle Parker Bryan T. Gnade Anthony R. Jones Christopher Whittier Kay Mereish
author_facet	Kenneth B. Yeh Hillary Wood Matt Scullion Joseph A. Russell Kyle Parker Bryan T. Gnade Anthony R. Jones Christopher Whittier Kay Mereish
author_sort	Kenneth B. Yeh
title	Molecular Detection of Biological Agents in the Field: Then and Now
title_short	Molecular Detection of Biological Agents in the Field: Then and Now
title_full	Molecular Detection of Biological Agents in the Field: Then and Now
title_fullStr	Molecular Detection of Biological Agents in the Field: Then and Now
title_full_unstemmed	Molecular Detection of Biological Agents in the Field: Then and Now
title_sort	molecular detection of biological agents in the field: then and now
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/e625aaf913c14b7fb1a7a243be7d30da
work_keys_str_mv	AT kennethbyeh moleculardetectionofbiologicalagentsinthefieldthenandnow AT hillarywood moleculardetectionofbiologicalagentsinthefieldthenandnow AT mattscullion moleculardetectionofbiologicalagentsinthefieldthenandnow AT josepharussell moleculardetectionofbiologicalagentsinthefieldthenandnow AT kyleparker moleculardetectionofbiologicalagentsinthefieldthenandnow AT bryantgnade moleculardetectionofbiologicalagentsinthefieldthenandnow AT anthonyrjones moleculardetectionofbiologicalagentsinthefieldthenandnow AT christopherwhittier moleculardetectionofbiologicalagentsinthefieldthenandnow AT kaymereish moleculardetectionofbiologicalagentsinthefieldthenandnow
_version_	1718428042346364928

Molecular Detection of Biological Agents in the Field: Then and Now

Ejemplares similares