Genomic data of an environmental Escherichia coli isolate shows high resemblance to E. coli K-12 reference strain MG1655.

Escherichia coli species exhibits a high genomic diversification from evolution, mobile genetic elements and recombination. An environmental E. coli isolate, ‘JHI_5025’ from a crop trial appeared to be clonally related to the historical reference isolate E. coli K-12 strain ‘MG1655’, warranting furt...

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Autor principal: Nicola Holden
Formato: article
Lenguaje:EN
Publicado: Elsevier 2021
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Acceso en línea:https://doaj.org/article/e627160477304a4db0275e36918dd10c
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Sumario:Escherichia coli species exhibits a high genomic diversification from evolution, mobile genetic elements and recombination. An environmental E. coli isolate, ‘JHI_5025’ from a crop trial appeared to be clonally related to the historical reference isolate E. coli K-12 strain ‘MG1655’, warranting further genomic analysis. Their genomes share an average nucleotide identity of 99.74% and whole genome alignment showed little rearrangement of the JHI_5025 sequence compared to the reference. Five genomic islands not in the reference aligned to other sequences in the Enterobacteriaceae. Isolate JHI_5025 contained E. coli K-12 F plasmid sequence and at least one complete prophage sequence. The genome and comparison dataset provides utility of E. coli JHI_5025 as a representative contemporary genetic mimic of a well-known and much used workhorse strain.