A LINK BETWEEN ACTIVE MYELOPEROXIDASE AND CHLORINATED CERULOPLASMIN IN BLOOD PLASMA OF PATIENTS WITH CARDIOVASCULAR DISEASES

Myeloperoxidase is a key factor promoting development of halogenative/oxidative stress under inflammatory conditions. Previously, we have discovered complexes including myeloperoxidase and its physiological inhibitor, ceruloplasmin in blood plasma of patients with inflammatory diseases of different...

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Autores principales:	A. V. Sokolov, V. A. Kostevich, N. V. Gorbunov, D. V. Grigorieva, I. V. Gorudko, V. B. Vasilyev, O. M. Panasenko
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2018
Materias:	myeloperoxidase halogenative stress ceruloplasmin peroxidase activity cardiovascular diseases biomarker Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/e627e47f30214d8aa883d46108f91c8a
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spelling	oai:doaj.org-article:e627e47f30214d8aa883d46108f91c8a2021-11-18T08:03:47ZA LINK BETWEEN ACTIVE MYELOPEROXIDASE AND CHLORINATED CERULOPLASMIN IN BLOOD PLASMA OF PATIENTS WITH CARDIOVASCULAR DISEASES1563-06252313-741X10.15789/1563-0625-2018-5-699-710https://doaj.org/article/e627e47f30214d8aa883d46108f91c8a2018-11-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1638https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XMyeloperoxidase is a key factor promoting development of halogenative/oxidative stress under inflammatory conditions. Previously, we have discovered complexes including myeloperoxidase and its physiological inhibitor, ceruloplasmin in blood plasma of patients with inflammatory diseases of different etiology, e.g., atherosclerosis. Studies on regulation of myeloperoxidase activity by ceruloplasmin have shown that hypochlorous acid, a specific product of myeloperoxidase action, is likely to modify ceruloplasmin during inflammation. The present study was aimed for analysis of relationships between the myeloperoxidase activity, native, and HOCl-modified ceruloplasmin levels in blood plasma samples of the patients with cardiovascular diseases.Specific antibodies against myeloperoxidase, ceruloplasmin, and HOCl-modified ceruloplasmin were obtained and specific enzyme-linked immunosorbent assays (ELISA) were developed. A combination of highly sensitive methods of myeloperoxidase assay i.e., solid-phase adsorption of antigens with subsequent testing of either their activity, or peroxidase-labeled antibody activity allowed elaborating the highly sensitive assays for ceruloplasmin and its HOCl-modified molecules, and for myeloperoxidase (concentration, peroxidase and halogenating activity). Positive correlation was proven between the myeloperoxidase concentration and activities. HOCl-modified ceruloplasmin content also correlated with myeloperoxidase activity.The HOCl-modified ceruloplasmin was first discovered in blood plasma samples from patients with cardiovascular diseases. In view of correlation between myeloperoxidase activity and HOCl-modified ceruloplasmin content in plasma, we suggest that HOCl production is aimed for suppression of myeloperoxidaseinhibitory function of ceruloplasmin.A. V. SokolovV. A. KostevichN. V. GorbunovD. V. GrigorievaI. V. GorudkoV. B. VasilyevO. M. PanasenkoSPb RAACIarticlemyeloperoxidasehalogenative stressceruloplasminperoxidase activitycardiovascular diseasesbiomarkerImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 20, Iss 5, Pp 699-710 (2018)
institution	DOAJ
collection	DOAJ
language	RU
topic	myeloperoxidase halogenative stress ceruloplasmin peroxidase activity cardiovascular diseases biomarker Immunologic diseases. Allergy RC581-607
spellingShingle	myeloperoxidase halogenative stress ceruloplasmin peroxidase activity cardiovascular diseases biomarker Immunologic diseases. Allergy RC581-607 A. V. Sokolov V. A. Kostevich N. V. Gorbunov D. V. Grigorieva I. V. Gorudko V. B. Vasilyev O. M. Panasenko A LINK BETWEEN ACTIVE MYELOPEROXIDASE AND CHLORINATED CERULOPLASMIN IN BLOOD PLASMA OF PATIENTS WITH CARDIOVASCULAR DISEASES
description	Myeloperoxidase is a key factor promoting development of halogenative/oxidative stress under inflammatory conditions. Previously, we have discovered complexes including myeloperoxidase and its physiological inhibitor, ceruloplasmin in blood plasma of patients with inflammatory diseases of different etiology, e.g., atherosclerosis. Studies on regulation of myeloperoxidase activity by ceruloplasmin have shown that hypochlorous acid, a specific product of myeloperoxidase action, is likely to modify ceruloplasmin during inflammation. The present study was aimed for analysis of relationships between the myeloperoxidase activity, native, and HOCl-modified ceruloplasmin levels in blood plasma samples of the patients with cardiovascular diseases.Specific antibodies against myeloperoxidase, ceruloplasmin, and HOCl-modified ceruloplasmin were obtained and specific enzyme-linked immunosorbent assays (ELISA) were developed. A combination of highly sensitive methods of myeloperoxidase assay i.e., solid-phase adsorption of antigens with subsequent testing of either their activity, or peroxidase-labeled antibody activity allowed elaborating the highly sensitive assays for ceruloplasmin and its HOCl-modified molecules, and for myeloperoxidase (concentration, peroxidase and halogenating activity). Positive correlation was proven between the myeloperoxidase concentration and activities. HOCl-modified ceruloplasmin content also correlated with myeloperoxidase activity.The HOCl-modified ceruloplasmin was first discovered in blood plasma samples from patients with cardiovascular diseases. In view of correlation between myeloperoxidase activity and HOCl-modified ceruloplasmin content in plasma, we suggest that HOCl production is aimed for suppression of myeloperoxidaseinhibitory function of ceruloplasmin.
format	article
author	A. V. Sokolov V. A. Kostevich N. V. Gorbunov D. V. Grigorieva I. V. Gorudko V. B. Vasilyev O. M. Panasenko
author_facet	A. V. Sokolov V. A. Kostevich N. V. Gorbunov D. V. Grigorieva I. V. Gorudko V. B. Vasilyev O. M. Panasenko
author_sort	A. V. Sokolov
title	A LINK BETWEEN ACTIVE MYELOPEROXIDASE AND CHLORINATED CERULOPLASMIN IN BLOOD PLASMA OF PATIENTS WITH CARDIOVASCULAR DISEASES
title_short	A LINK BETWEEN ACTIVE MYELOPEROXIDASE AND CHLORINATED CERULOPLASMIN IN BLOOD PLASMA OF PATIENTS WITH CARDIOVASCULAR DISEASES
title_full	A LINK BETWEEN ACTIVE MYELOPEROXIDASE AND CHLORINATED CERULOPLASMIN IN BLOOD PLASMA OF PATIENTS WITH CARDIOVASCULAR DISEASES
title_fullStr	A LINK BETWEEN ACTIVE MYELOPEROXIDASE AND CHLORINATED CERULOPLASMIN IN BLOOD PLASMA OF PATIENTS WITH CARDIOVASCULAR DISEASES
title_full_unstemmed	A LINK BETWEEN ACTIVE MYELOPEROXIDASE AND CHLORINATED CERULOPLASMIN IN BLOOD PLASMA OF PATIENTS WITH CARDIOVASCULAR DISEASES
title_sort	link between active myeloperoxidase and chlorinated ceruloplasmin in blood plasma of patients with cardiovascular diseases
publisher	SPb RAACI
publishDate	2018
url	https://doaj.org/article/e627e47f30214d8aa883d46108f91c8a
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A LINK BETWEEN ACTIVE MYELOPEROXIDASE AND CHLORINATED CERULOPLASMIN IN BLOOD PLASMA OF PATIENTS WITH CARDIOVASCULAR DISEASES

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