Selective targeting of ligand-dependent and -independent signaling by GPCR conformation-specific anti-US28 intrabodies

Various GPCRs display constitutive ligand-independent activity, but it remains unclear whether ligand-dependent and -independent conformations differ. Here the authors demonstrate the recognition and blocking of G protein recruitment of either the ligand-bound active, or the constitutively active ap...

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Autores principales:	Timo W. M. De Groof, Nick D. Bergkamp, Raimond Heukers, Truc Giap, Maarten P. Bebelman, Richard Goeij-de Haas, Sander R. Piersma, Connie R. Jimenez, K. Christopher Garcia, Hidde L. Ploegh, Marco Siderius, Martine J. Smit
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/e627ed6b73f44763bda4541f3d3a971b
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spelling	oai:doaj.org-article:e627ed6b73f44763bda4541f3d3a971b2021-12-02T17:01:29ZSelective targeting of ligand-dependent and -independent signaling by GPCR conformation-specific anti-US28 intrabodies10.1038/s41467-021-24574-y2041-1723https://doaj.org/article/e627ed6b73f44763bda4541f3d3a971b2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-24574-yhttps://doaj.org/toc/2041-1723Various GPCRs display constitutive ligand-independent activity, but it remains unclear whether ligand-dependent and -independent conformations differ. Here the authors demonstrate the recognition and blocking of G protein recruitment of either the ligand-bound active, or the constitutively active apo-conformation of the viral GPCR US28 by different nanobodies that target similar intracellular loops of the receptor.Timo W. M. De GroofNick D. BergkampRaimond HeukersTruc GiapMaarten P. BebelmanRichard Goeij-de HaasSander R. PiersmaConnie R. JimenezK. Christopher GarciaHidde L. PloeghMarco SideriusMartine J. SmitNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Timo W. M. De Groof Nick D. Bergkamp Raimond Heukers Truc Giap Maarten P. Bebelman Richard Goeij-de Haas Sander R. Piersma Connie R. Jimenez K. Christopher Garcia Hidde L. Ploegh Marco Siderius Martine J. Smit Selective targeting of ligand-dependent and -independent signaling by GPCR conformation-specific anti-US28 intrabodies
description	Various GPCRs display constitutive ligand-independent activity, but it remains unclear whether ligand-dependent and -independent conformations differ. Here the authors demonstrate the recognition and blocking of G protein recruitment of either the ligand-bound active, or the constitutively active apo-conformation of the viral GPCR US28 by different nanobodies that target similar intracellular loops of the receptor.
format	article
author	Timo W. M. De Groof Nick D. Bergkamp Raimond Heukers Truc Giap Maarten P. Bebelman Richard Goeij-de Haas Sander R. Piersma Connie R. Jimenez K. Christopher Garcia Hidde L. Ploegh Marco Siderius Martine J. Smit
author_facet	Timo W. M. De Groof Nick D. Bergkamp Raimond Heukers Truc Giap Maarten P. Bebelman Richard Goeij-de Haas Sander R. Piersma Connie R. Jimenez K. Christopher Garcia Hidde L. Ploegh Marco Siderius Martine J. Smit
author_sort	Timo W. M. De Groof
title	Selective targeting of ligand-dependent and -independent signaling by GPCR conformation-specific anti-US28 intrabodies
title_short	Selective targeting of ligand-dependent and -independent signaling by GPCR conformation-specific anti-US28 intrabodies
title_full	Selective targeting of ligand-dependent and -independent signaling by GPCR conformation-specific anti-US28 intrabodies
title_fullStr	Selective targeting of ligand-dependent and -independent signaling by GPCR conformation-specific anti-US28 intrabodies
title_full_unstemmed	Selective targeting of ligand-dependent and -independent signaling by GPCR conformation-specific anti-US28 intrabodies
title_sort	selective targeting of ligand-dependent and -independent signaling by gpcr conformation-specific anti-us28 intrabodies
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e627ed6b73f44763bda4541f3d3a971b
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Selective targeting of ligand-dependent and -independent signaling by GPCR conformation-specific anti-US28 intrabodies

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