Sleep deprivation does not affect neuronal susceptibility to mild traumatic brain injury in the rat

Aimee M Caron, Richard Stephenson Department of Cell and Systems Biology, University of Toronto, Toronto, ON, Canada Abstract: Mild and moderate traumatic brain injuries (TBIs) (and concussion) occur frequently as a result of falls, automobile accidents, and sporting activities, and are a major cau...

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Autores principales:	Caron AM, Stephenson R
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Psychiatry RC435-571 Neurophysiology and neuropsychology QP351-495
Acceso en línea:	https://doaj.org/article/e62f28433cda41e2bb1ee88f8b20b404
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spelling	oai:doaj.org-article:e62f28433cda41e2bb1ee88f8b20b4042021-12-02T01:16:09ZSleep deprivation does not affect neuronal susceptibility to mild traumatic brain injury in the rat1179-1608https://doaj.org/article/e62f28433cda41e2bb1ee88f8b20b4042015-06-01T00:00:00Zhttp://www.dovepress.com/sleep-deprivation-does-not-affect-neuronal-susceptibility-to-mild-trau-peer-reviewed-article-NSShttps://doaj.org/toc/1179-1608Aimee M Caron, Richard Stephenson Department of Cell and Systems Biology, University of Toronto, Toronto, ON, Canada Abstract: Mild and moderate traumatic brain injuries (TBIs) (and concussion) occur frequently as a result of falls, automobile accidents, and sporting activities, and are a major cause of acute and chronic disability. Fatigue and excessive sleepiness are associated with increased risk of accidents, but it is unknown whether prior sleep debt also affects the pathophysiological outcome of concussive injury. Using the “dark neuron” (DN) as a marker of reversible neuronal damage, we tested the hypothesis that acute (48 hours) total sleep deprivation (TSD) and chronic sleep restriction (CSR; 10 days, 6-hour sleep/day) affect DN formation following mild TBI in the rat. TSD and CSR were administered using a walking wheel apparatus. Mild TBI was administered under anesthesia using a weight-drop impact model, and the acute neuronal response was observed without recovery. DNs were detected using standard bright-field microscopy with toluidine blue stain following appropriate tissue fixation. DN density was low under home cage and sleep deprivation control conditions (respective median DN densities, 0.14% and 0.22% of neurons), and this was unaffected by TSD alone (0.1%). Mild TBI caused significantly higher DN densities (0.76%), and this was unchanged by preexisting acute or chronic sleep debt (TSD, 0.23%; CSR, 0.7%). Thus, although sleep debt may be predicted to increase the incidence of concussive injury, the present data suggest that sleep debt does not exacerbate the resulting neuronal damage. Keywords: sleep deprivation, concussion, traumatic brain injury, dark neuron, neurodegeneration, rat cortexCaron AMStephenson RDove Medical PressarticlePsychiatryRC435-571Neurophysiology and neuropsychologyQP351-495ENNature and Science of Sleep, Vol 2015, Iss default, Pp 63-72 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Psychiatry RC435-571 Neurophysiology and neuropsychology QP351-495
spellingShingle	Psychiatry RC435-571 Neurophysiology and neuropsychology QP351-495 Caron AM Stephenson R Sleep deprivation does not affect neuronal susceptibility to mild traumatic brain injury in the rat
description	Aimee M Caron, Richard Stephenson Department of Cell and Systems Biology, University of Toronto, Toronto, ON, Canada Abstract: Mild and moderate traumatic brain injuries (TBIs) (and concussion) occur frequently as a result of falls, automobile accidents, and sporting activities, and are a major cause of acute and chronic disability. Fatigue and excessive sleepiness are associated with increased risk of accidents, but it is unknown whether prior sleep debt also affects the pathophysiological outcome of concussive injury. Using the “dark neuron” (DN) as a marker of reversible neuronal damage, we tested the hypothesis that acute (48 hours) total sleep deprivation (TSD) and chronic sleep restriction (CSR; 10 days, 6-hour sleep/day) affect DN formation following mild TBI in the rat. TSD and CSR were administered using a walking wheel apparatus. Mild TBI was administered under anesthesia using a weight-drop impact model, and the acute neuronal response was observed without recovery. DNs were detected using standard bright-field microscopy with toluidine blue stain following appropriate tissue fixation. DN density was low under home cage and sleep deprivation control conditions (respective median DN densities, 0.14% and 0.22% of neurons), and this was unaffected by TSD alone (0.1%). Mild TBI caused significantly higher DN densities (0.76%), and this was unchanged by preexisting acute or chronic sleep debt (TSD, 0.23%; CSR, 0.7%). Thus, although sleep debt may be predicted to increase the incidence of concussive injury, the present data suggest that sleep debt does not exacerbate the resulting neuronal damage. Keywords: sleep deprivation, concussion, traumatic brain injury, dark neuron, neurodegeneration, rat cortex
format	article
author	Caron AM Stephenson R
author_facet	Caron AM Stephenson R
author_sort	Caron AM
title	Sleep deprivation does not affect neuronal susceptibility to mild traumatic brain injury in the rat
title_short	Sleep deprivation does not affect neuronal susceptibility to mild traumatic brain injury in the rat
title_full	Sleep deprivation does not affect neuronal susceptibility to mild traumatic brain injury in the rat
title_fullStr	Sleep deprivation does not affect neuronal susceptibility to mild traumatic brain injury in the rat
title_full_unstemmed	Sleep deprivation does not affect neuronal susceptibility to mild traumatic brain injury in the rat
title_sort	sleep deprivation does not affect neuronal susceptibility to mild traumatic brain injury in the rat
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/e62f28433cda41e2bb1ee88f8b20b404
work_keys_str_mv	AT caronam sleepdeprivationdoesnotaffectneuronalsusceptibilitytomildtraumaticbraininjuryintherat AT stephensonr sleepdeprivationdoesnotaffectneuronalsusceptibilitytomildtraumaticbraininjuryintherat
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Sleep deprivation does not affect neuronal susceptibility to mild traumatic brain injury in the rat

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