ANTI-APOPTOTIC EFFECT OF CD95 RECEPTOR IN NA VE CD8+ T-LYMPHOCYTES IN CHILDREN WITH ACUTE INFECTIOUS MONONUCLEOSIS

Acute infectious mononucleosis is a widespread viral disease, which most often manifests in childhood. The development of acute infectious mononucleosis is accompanied by the change of the CD4+/CD8+ T-lymphocytes ratio and the increase of the virus-specific CD8+ cytotoxic T-lymphocytes number. One o...

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Autores principales:	E. N. Filatova, E. V. Anisenkova, N. B. Presnyakova, T. D. Sycheva, E. A. Kulova, O. V. Utkin
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2016
Materias:	cd95, apoptosis, naïve t-lymphocytes, cd4, cd8, acute infectious mononucleosis Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/e635a9e43dc44dafacfa9b2b65b801e9
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spelling	oai:doaj.org-article:e635a9e43dc44dafacfa9b2b65b801e92021-11-22T07:09:48ZANTI-APOPTOTIC EFFECT OF CD95 RECEPTOR IN NA VE CD8+ T-LYMPHOCYTES IN CHILDREN WITH ACUTE INFECTIOUS MONONUCLEOSIS2220-76192313-739810.15789/2220-7619-2016-3-207-218https://doaj.org/article/e635a9e43dc44dafacfa9b2b65b801e92016-09-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/420https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398Acute infectious mononucleosis is a widespread viral disease, which most often manifests in childhood. The development of acute infectious mononucleosis is accompanied by the change of the CD4+/CD8+ T-lymphocytes ratio and the increase of the virus-specific CD8+ cytotoxic T-lymphocytes number. One of the T-lymphocytes number regulation mechanisms is the modulation of their progenitor cells apoptosis. The death receptor CD95 takes part in the regulation of T-lymphocytes apoptosis, including naïve T-cells. We studied the effect of CD95 receptor activation on apoptosis of naïve CD4+ and naïve cytotoxic CD8+ T-lymphocytes in healthy children and children with acute infectious mononucleosis. In this study children with acute infectious mononucleosis at the age of 9 to 16 years were included. For comparison healthy children of the same age with no clinical and laboratory signs of the disease were used. Naïve CD4+ and naïve cytotoxic CD8+ T-lymphocytes were isolated by negative magnetic immunoseparation. The analysis of naïve T-cells apoptosis and the CD95 receptor surface expression density was performed by using the flow cytometry analysis. The analysis of T-cells was performed in three variants: freshly isolated naïve CD4+ T-lymphocytes and naïve cytotoxic CD8+ T-lymphocytes, and also cells after 24 hours of the cultivation with anti-CD95 monoclonal antibodies or without them. In healthy children both CD95– and CD95+ naïve CD4+ T-lymphocytes underwent apoptosis. In children with acute infectious mononucleosis CD95– naïve CD4+ T-lymphocytes lost their susceptibility to apoptosis induction. In healthy children and children with acute infectious mononucleosis CD95– naïve cytotoxic CD8+ T-lymphocytes were resistant to apoptosis in contrast to CD95+ naïve CD4+ T-lymphocytes. In healthy children CD95 receptor did not induce apoptosis of isolated naïve CD4+ T-lymphocytes and naïve cytotoxic CD8+ T-lymphocytes. In children with acute infectious mononucleosis CD95 receptor was involved in inhibition of apoptosis of naïve cytotoxic CD8+ T-lymphocytes and did not effect on the level of apoptosis of naïve CD4+ T-lymphocytes. We suggest that CD95-dependent suppression of naïve cytotoxic CD8+ T-lymphocytes apoptosis is a protective mechanism for the maintenance of a sufficient number of cytotoxic T-lymphocytes in the blood for the realization of effective antiviral immune response.E. N. FilatovaE. V. AnisenkovaN. B. PresnyakovaT. D. SychevaE. A. KulovaO. V. UtkinSankt-Peterburg : NIIÈM imeni Pasteraarticlecd95, apoptosis, naïve t-lymphocytes, cd4, cd8, acute infectious mononucleosisInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 6, Iss 3, Pp 207-218 (2016)
institution	DOAJ
collection	DOAJ
language	RU
topic	cd95, apoptosis, naïve t-lymphocytes, cd4, cd8, acute infectious mononucleosis Infectious and parasitic diseases RC109-216
spellingShingle	cd95, apoptosis, naïve t-lymphocytes, cd4, cd8, acute infectious mononucleosis Infectious and parasitic diseases RC109-216 E. N. Filatova E. V. Anisenkova N. B. Presnyakova T. D. Sycheva E. A. Kulova O. V. Utkin ANTI-APOPTOTIC EFFECT OF CD95 RECEPTOR IN NA VE CD8+ T-LYMPHOCYTES IN CHILDREN WITH ACUTE INFECTIOUS MONONUCLEOSIS
description	Acute infectious mononucleosis is a widespread viral disease, which most often manifests in childhood. The development of acute infectious mononucleosis is accompanied by the change of the CD4+/CD8+ T-lymphocytes ratio and the increase of the virus-specific CD8+ cytotoxic T-lymphocytes number. One of the T-lymphocytes number regulation mechanisms is the modulation of their progenitor cells apoptosis. The death receptor CD95 takes part in the regulation of T-lymphocytes apoptosis, including naïve T-cells. We studied the effect of CD95 receptor activation on apoptosis of naïve CD4+ and naïve cytotoxic CD8+ T-lymphocytes in healthy children and children with acute infectious mononucleosis. In this study children with acute infectious mononucleosis at the age of 9 to 16 years were included. For comparison healthy children of the same age with no clinical and laboratory signs of the disease were used. Naïve CD4+ and naïve cytotoxic CD8+ T-lymphocytes were isolated by negative magnetic immunoseparation. The analysis of naïve T-cells apoptosis and the CD95 receptor surface expression density was performed by using the flow cytometry analysis. The analysis of T-cells was performed in three variants: freshly isolated naïve CD4+ T-lymphocytes and naïve cytotoxic CD8+ T-lymphocytes, and also cells after 24 hours of the cultivation with anti-CD95 monoclonal antibodies or without them. In healthy children both CD95– and CD95+ naïve CD4+ T-lymphocytes underwent apoptosis. In children with acute infectious mononucleosis CD95– naïve CD4+ T-lymphocytes lost their susceptibility to apoptosis induction. In healthy children and children with acute infectious mononucleosis CD95– naïve cytotoxic CD8+ T-lymphocytes were resistant to apoptosis in contrast to CD95+ naïve CD4+ T-lymphocytes. In healthy children CD95 receptor did not induce apoptosis of isolated naïve CD4+ T-lymphocytes and naïve cytotoxic CD8+ T-lymphocytes. In children with acute infectious mononucleosis CD95 receptor was involved in inhibition of apoptosis of naïve cytotoxic CD8+ T-lymphocytes and did not effect on the level of apoptosis of naïve CD4+ T-lymphocytes. We suggest that CD95-dependent suppression of naïve cytotoxic CD8+ T-lymphocytes apoptosis is a protective mechanism for the maintenance of a sufficient number of cytotoxic T-lymphocytes in the blood for the realization of effective antiviral immune response.
format	article
author	E. N. Filatova E. V. Anisenkova N. B. Presnyakova T. D. Sycheva E. A. Kulova O. V. Utkin
author_facet	E. N. Filatova E. V. Anisenkova N. B. Presnyakova T. D. Sycheva E. A. Kulova O. V. Utkin
author_sort	E. N. Filatova
title	ANTI-APOPTOTIC EFFECT OF CD95 RECEPTOR IN NA VE CD8+ T-LYMPHOCYTES IN CHILDREN WITH ACUTE INFECTIOUS MONONUCLEOSIS
title_short	ANTI-APOPTOTIC EFFECT OF CD95 RECEPTOR IN NA VE CD8+ T-LYMPHOCYTES IN CHILDREN WITH ACUTE INFECTIOUS MONONUCLEOSIS
title_full	ANTI-APOPTOTIC EFFECT OF CD95 RECEPTOR IN NA VE CD8+ T-LYMPHOCYTES IN CHILDREN WITH ACUTE INFECTIOUS MONONUCLEOSIS
title_fullStr	ANTI-APOPTOTIC EFFECT OF CD95 RECEPTOR IN NA VE CD8+ T-LYMPHOCYTES IN CHILDREN WITH ACUTE INFECTIOUS MONONUCLEOSIS
title_full_unstemmed	ANTI-APOPTOTIC EFFECT OF CD95 RECEPTOR IN NA VE CD8+ T-LYMPHOCYTES IN CHILDREN WITH ACUTE INFECTIOUS MONONUCLEOSIS
title_sort	anti-apoptotic effect of cd95 receptor in na ve cd8+ t-lymphocytes in children with acute infectious mononucleosis
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2016
url	https://doaj.org/article/e635a9e43dc44dafacfa9b2b65b801e9
work_keys_str_mv	AT enfilatova antiapoptoticeffectofcd95receptorinnavecd8tlymphocytesinchildrenwithacuteinfectiousmononucleosis AT evanisenkova antiapoptoticeffectofcd95receptorinnavecd8tlymphocytesinchildrenwithacuteinfectiousmononucleosis AT nbpresnyakova antiapoptoticeffectofcd95receptorinnavecd8tlymphocytesinchildrenwithacuteinfectiousmononucleosis AT tdsycheva antiapoptoticeffectofcd95receptorinnavecd8tlymphocytesinchildrenwithacuteinfectiousmononucleosis AT eakulova antiapoptoticeffectofcd95receptorinnavecd8tlymphocytesinchildrenwithacuteinfectiousmononucleosis AT ovutkin antiapoptoticeffectofcd95receptorinnavecd8tlymphocytesinchildrenwithacuteinfectiousmononucleosis
_version_	1718418011007746048

ANTI-APOPTOTIC EFFECT OF CD95 RECEPTOR IN NA VE CD8+ T-LYMPHOCYTES IN CHILDREN WITH ACUTE INFECTIOUS MONONUCLEOSIS

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