Exosomes released from pancreatic cancer cells enhance angiogenic activities via dynamin-dependent endocytosis in endothelial cells in vitro

Abstract Pancreatic cancer has the lowest 5 year survival rate among all cancers. Several extracellular factors are involved in the development and metastasis of pancreatic cancer to distant organs. Exosomes are lipid-bilayer, membrane-enclosed nanoparticles that are recognised as important mediator...

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Autores principales:	Mitsuru Chiba, Shiori Kubota, Konomi Sato, Satoru Monzen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e63701c5c6754e99b1eea35e6e54c133
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spelling	oai:doaj.org-article:e63701c5c6754e99b1eea35e6e54c1332021-12-02T15:08:16ZExosomes released from pancreatic cancer cells enhance angiogenic activities via dynamin-dependent endocytosis in endothelial cells in vitro10.1038/s41598-018-30446-12045-2322https://doaj.org/article/e63701c5c6754e99b1eea35e6e54c1332018-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-30446-1https://doaj.org/toc/2045-2322Abstract Pancreatic cancer has the lowest 5 year survival rate among all cancers. Several extracellular factors are involved in the development and metastasis of pancreatic cancer to distant organs. Exosomes are lipid-bilayer, membrane-enclosed nanoparticles that are recognised as important mediators of cell-to-cell communications. However, the role of exosomes released from pancreatic cancer cells in tumour micro-environment remains unknown. Here, we show that exosomes released from pancreatic cancer PK-45H cells activate various gene expressions in human umbilical vein endothelial cells (HUVECs) by in vitro analyses. In addition, these exosomes released from PK-45H cells promote phosphorylation of Akt and ERK1/2 signalling pathway molecules and tube formation via dynamin-dependent endocytosis in HUVECs. Our findings suggested that exosomes released from pancreatic cancer cells may act as a novel angiogenesis promoter.Mitsuru ChibaShiori KubotaKonomi SatoSatoru MonzenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mitsuru Chiba Shiori Kubota Konomi Sato Satoru Monzen Exosomes released from pancreatic cancer cells enhance angiogenic activities via dynamin-dependent endocytosis in endothelial cells in vitro
description	Abstract Pancreatic cancer has the lowest 5 year survival rate among all cancers. Several extracellular factors are involved in the development and metastasis of pancreatic cancer to distant organs. Exosomes are lipid-bilayer, membrane-enclosed nanoparticles that are recognised as important mediators of cell-to-cell communications. However, the role of exosomes released from pancreatic cancer cells in tumour micro-environment remains unknown. Here, we show that exosomes released from pancreatic cancer PK-45H cells activate various gene expressions in human umbilical vein endothelial cells (HUVECs) by in vitro analyses. In addition, these exosomes released from PK-45H cells promote phosphorylation of Akt and ERK1/2 signalling pathway molecules and tube formation via dynamin-dependent endocytosis in HUVECs. Our findings suggested that exosomes released from pancreatic cancer cells may act as a novel angiogenesis promoter.
format	article
author	Mitsuru Chiba Shiori Kubota Konomi Sato Satoru Monzen
author_facet	Mitsuru Chiba Shiori Kubota Konomi Sato Satoru Monzen
author_sort	Mitsuru Chiba
title	Exosomes released from pancreatic cancer cells enhance angiogenic activities via dynamin-dependent endocytosis in endothelial cells in vitro
title_short	Exosomes released from pancreatic cancer cells enhance angiogenic activities via dynamin-dependent endocytosis in endothelial cells in vitro
title_full	Exosomes released from pancreatic cancer cells enhance angiogenic activities via dynamin-dependent endocytosis in endothelial cells in vitro
title_fullStr	Exosomes released from pancreatic cancer cells enhance angiogenic activities via dynamin-dependent endocytosis in endothelial cells in vitro
title_full_unstemmed	Exosomes released from pancreatic cancer cells enhance angiogenic activities via dynamin-dependent endocytosis in endothelial cells in vitro
title_sort	exosomes released from pancreatic cancer cells enhance angiogenic activities via dynamin-dependent endocytosis in endothelial cells in vitro
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/e63701c5c6754e99b1eea35e6e54c133
work_keys_str_mv	AT mitsuruchiba exosomesreleasedfrompancreaticcancercellsenhanceangiogenicactivitiesviadynamindependentendocytosisinendothelialcellsinvitro AT shiorikubota exosomesreleasedfrompancreaticcancercellsenhanceangiogenicactivitiesviadynamindependentendocytosisinendothelialcellsinvitro AT konomisato exosomesreleasedfrompancreaticcancercellsenhanceangiogenicactivitiesviadynamindependentendocytosisinendothelialcellsinvitro AT satorumonzen exosomesreleasedfrompancreaticcancercellsenhanceangiogenicactivitiesviadynamindependentendocytosisinendothelialcellsinvitro
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Exosomes released from pancreatic cancer cells enhance angiogenic activities via dynamin-dependent endocytosis in endothelial cells in vitro

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