Assessment of microbial diversity in biofilms recovered from endotracheal tubes using culture dependent and independent approaches.

Ventilator-associated pneumonia (VAP) is a common nosocomial infection in mechanically ventilated patients. Biofilm formation is one of the mechanisms through which the endotracheal tube (ET) facilitates bacterial contamination of the lower airways. In the present study, we analyzed the composition...

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Autores principales: Ilse Vandecandelaere, Nele Matthijs, Filip Van Nieuwerburgh, Dieter Deforce, Peter Vosters, Liesbet De Bus, Hans J Nelis, Pieter Depuydt, Tom Coenye
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Publicado: Public Library of Science (PLoS) 2012
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spelling oai:doaj.org-article:e6386334d8b949dfaab717a27acb80022021-11-18T07:16:18ZAssessment of microbial diversity in biofilms recovered from endotracheal tubes using culture dependent and independent approaches.1932-620310.1371/journal.pone.0038401https://doaj.org/article/e6386334d8b949dfaab717a27acb80022012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22693635/?tool=EBIhttps://doaj.org/toc/1932-6203Ventilator-associated pneumonia (VAP) is a common nosocomial infection in mechanically ventilated patients. Biofilm formation is one of the mechanisms through which the endotracheal tube (ET) facilitates bacterial contamination of the lower airways. In the present study, we analyzed the composition of the ET biofilm flora by means of culture dependent and culture independent (16 S rRNA gene clone libraries and pyrosequencing) approaches. Overall, the microbial diversity was high and members of different phylogenetic lineages were detected (Actinobacteria, beta-Proteobacteria, Candida spp., Clostridia, epsilon-Proteobacteria, Firmicutes, Fusobacteria and gamma-Proteobacteria). Culture dependent analysis, based on the use of selective growth media and conventional microbiological tests, resulted in the identification of typical aerobic nosocomial pathogens which are known to play a role in the development of VAP, e.g. Staphylococcus aureus and Pseudomonas aeruginosa. Other opportunistic pathogens were also identified, including Staphylococcus epidermidis and Kocuria varians. In general, there was little correlation between the results obtained by sequencing 16 S rRNA gene clone libraries and by cultivation. Pyrosequencing of PCR amplified 16 S rRNA genes of four selected samples resulted in the identification of a much wider variety of bacteria. The results from the pyrosequencing analysis suggest that these four samples were dominated by members of the normal oral flora such as Prevotella spp., Peptostreptococcus spp. and lactic acid bacteria. A combination of methods is recommended to obtain a complete picture of the microbial diversity of the ET biofilm.Ilse VandecandelaereNele MatthijsFilip Van NieuwerburghDieter DeforcePeter VostersLiesbet De BusHans J NelisPieter DepuydtTom CoenyePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 6, p e38401 (2012)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Ilse Vandecandelaere
Nele Matthijs
Filip Van Nieuwerburgh
Dieter Deforce
Peter Vosters
Liesbet De Bus
Hans J Nelis
Pieter Depuydt
Tom Coenye
Assessment of microbial diversity in biofilms recovered from endotracheal tubes using culture dependent and independent approaches.
description Ventilator-associated pneumonia (VAP) is a common nosocomial infection in mechanically ventilated patients. Biofilm formation is one of the mechanisms through which the endotracheal tube (ET) facilitates bacterial contamination of the lower airways. In the present study, we analyzed the composition of the ET biofilm flora by means of culture dependent and culture independent (16 S rRNA gene clone libraries and pyrosequencing) approaches. Overall, the microbial diversity was high and members of different phylogenetic lineages were detected (Actinobacteria, beta-Proteobacteria, Candida spp., Clostridia, epsilon-Proteobacteria, Firmicutes, Fusobacteria and gamma-Proteobacteria). Culture dependent analysis, based on the use of selective growth media and conventional microbiological tests, resulted in the identification of typical aerobic nosocomial pathogens which are known to play a role in the development of VAP, e.g. Staphylococcus aureus and Pseudomonas aeruginosa. Other opportunistic pathogens were also identified, including Staphylococcus epidermidis and Kocuria varians. In general, there was little correlation between the results obtained by sequencing 16 S rRNA gene clone libraries and by cultivation. Pyrosequencing of PCR amplified 16 S rRNA genes of four selected samples resulted in the identification of a much wider variety of bacteria. The results from the pyrosequencing analysis suggest that these four samples were dominated by members of the normal oral flora such as Prevotella spp., Peptostreptococcus spp. and lactic acid bacteria. A combination of methods is recommended to obtain a complete picture of the microbial diversity of the ET biofilm.
format article
author Ilse Vandecandelaere
Nele Matthijs
Filip Van Nieuwerburgh
Dieter Deforce
Peter Vosters
Liesbet De Bus
Hans J Nelis
Pieter Depuydt
Tom Coenye
author_facet Ilse Vandecandelaere
Nele Matthijs
Filip Van Nieuwerburgh
Dieter Deforce
Peter Vosters
Liesbet De Bus
Hans J Nelis
Pieter Depuydt
Tom Coenye
author_sort Ilse Vandecandelaere
title Assessment of microbial diversity in biofilms recovered from endotracheal tubes using culture dependent and independent approaches.
title_short Assessment of microbial diversity in biofilms recovered from endotracheal tubes using culture dependent and independent approaches.
title_full Assessment of microbial diversity in biofilms recovered from endotracheal tubes using culture dependent and independent approaches.
title_fullStr Assessment of microbial diversity in biofilms recovered from endotracheal tubes using culture dependent and independent approaches.
title_full_unstemmed Assessment of microbial diversity in biofilms recovered from endotracheal tubes using culture dependent and independent approaches.
title_sort assessment of microbial diversity in biofilms recovered from endotracheal tubes using culture dependent and independent approaches.
publisher Public Library of Science (PLoS)
publishDate 2012
url https://doaj.org/article/e6386334d8b949dfaab717a27acb8002
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