Photoswitchable single-stranded DNA-peptide coacervate formation as a dynamic system for reaction control

Summary: In cells, segregation allows for diverse biochemical reactions to take place simultaneously. Such intricate regulation of cellular processes is achieved through the dynamic formation and disassembly of membraneless organelles via liquid-liquid phase separation (LLPS). Herein, we demonstrate...

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Autores principales:	Wen Ann Wee, Hiroshi Sugiyama, Soyoung Park
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Imaging methods in chemistry Biochemistry Cell biology Science Q
Acceso en línea:	https://doaj.org/article/e647d2a2a64443a68a1563f69f792541
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spelling	oai:doaj.org-article:e647d2a2a64443a68a1563f69f7925412021-11-28T04:36:45ZPhotoswitchable single-stranded DNA-peptide coacervate formation as a dynamic system for reaction control2589-004210.1016/j.isci.2021.103455https://doaj.org/article/e647d2a2a64443a68a1563f69f7925412021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2589004221014267https://doaj.org/toc/2589-0042Summary: In cells, segregation allows for diverse biochemical reactions to take place simultaneously. Such intricate regulation of cellular processes is achieved through the dynamic formation and disassembly of membraneless organelles via liquid-liquid phase separation (LLPS). Herein, we demonstrate the light-controlled formation and disassembly of liquid droplets formed from a complex of polylysine (pLys) and arylazopyrazole (AAP)-conjugated single-stranded DNA. Photoswitchablility of droplet formation was also shown to be applicable to the control of chemical reactions; imine formation and a DNAzyme-catalyzed oxidation reaction were accelerated in the presence of droplets. These outcomes were reversed upon droplet disassembly. Our results demonstrate that the photoswitchable droplet formation system is a versatile model for the regulation of reactions through dynamic LLPS.Wen Ann WeeHiroshi SugiyamaSoyoung ParkElsevierarticleImaging methods in chemistryBiochemistryCell biologyScienceQENiScience, Vol 24, Iss 12, Pp 103455- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Imaging methods in chemistry Biochemistry Cell biology Science Q
spellingShingle	Imaging methods in chemistry Biochemistry Cell biology Science Q Wen Ann Wee Hiroshi Sugiyama Soyoung Park Photoswitchable single-stranded DNA-peptide coacervate formation as a dynamic system for reaction control
description	Summary: In cells, segregation allows for diverse biochemical reactions to take place simultaneously. Such intricate regulation of cellular processes is achieved through the dynamic formation and disassembly of membraneless organelles via liquid-liquid phase separation (LLPS). Herein, we demonstrate the light-controlled formation and disassembly of liquid droplets formed from a complex of polylysine (pLys) and arylazopyrazole (AAP)-conjugated single-stranded DNA. Photoswitchablility of droplet formation was also shown to be applicable to the control of chemical reactions; imine formation and a DNAzyme-catalyzed oxidation reaction were accelerated in the presence of droplets. These outcomes were reversed upon droplet disassembly. Our results demonstrate that the photoswitchable droplet formation system is a versatile model for the regulation of reactions through dynamic LLPS.
format	article
author	Wen Ann Wee Hiroshi Sugiyama Soyoung Park
author_facet	Wen Ann Wee Hiroshi Sugiyama Soyoung Park
author_sort	Wen Ann Wee
title	Photoswitchable single-stranded DNA-peptide coacervate formation as a dynamic system for reaction control
title_short	Photoswitchable single-stranded DNA-peptide coacervate formation as a dynamic system for reaction control
title_full	Photoswitchable single-stranded DNA-peptide coacervate formation as a dynamic system for reaction control
title_fullStr	Photoswitchable single-stranded DNA-peptide coacervate formation as a dynamic system for reaction control
title_full_unstemmed	Photoswitchable single-stranded DNA-peptide coacervate formation as a dynamic system for reaction control
title_sort	photoswitchable single-stranded dna-peptide coacervate formation as a dynamic system for reaction control
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/e647d2a2a64443a68a1563f69f792541
work_keys_str_mv	AT wenannwee photoswitchablesinglestrandeddnapeptidecoacervateformationasadynamicsystemforreactioncontrol AT hiroshisugiyama photoswitchablesinglestrandeddnapeptidecoacervateformationasadynamicsystemforreactioncontrol AT soyoungpark photoswitchablesinglestrandeddnapeptidecoacervateformationasadynamicsystemforreactioncontrol
_version_	1718408291888922624

Photoswitchable single-stranded DNA-peptide coacervate formation as a dynamic system for reaction control

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