Full-length transcriptome reconstruction reveals a large diversity of RNA and protein isoforms in rat hippocampus

It is challenging to characterize diverse transcript isoforms by short-read sequencing. Here the authors report full-length transcriptomes in rat hippocampus by hybrid-sequencing, predict isoform-specific translational status, and reconstruct open reading frames validated by mass spectrometry.

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Autores principales:	Xi Wang, Xintian You, Julian D. Langer, Jingyi Hou, Fiona Rupprecht, Irena Vlatkovic, Claudia Quedenau, Georgi Tushev, Irina Epstein, Bernhard Schaefke, Wei Sun, Liang Fang, Guipeng Li, Yuhui Hu, Erin M. Schuman, Wei Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/e648ad9d85e9487fb44af5a9caede55c
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spelling	oai:doaj.org-article:e648ad9d85e9487fb44af5a9caede55c2021-12-02T17:31:22ZFull-length transcriptome reconstruction reveals a large diversity of RNA and protein isoforms in rat hippocampus10.1038/s41467-019-13037-02041-1723https://doaj.org/article/e648ad9d85e9487fb44af5a9caede55c2019-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-13037-0https://doaj.org/toc/2041-1723It is challenging to characterize diverse transcript isoforms by short-read sequencing. Here the authors report full-length transcriptomes in rat hippocampus by hybrid-sequencing, predict isoform-specific translational status, and reconstruct open reading frames validated by mass spectrometry.Xi WangXintian YouJulian D. LangerJingyi HouFiona RupprechtIrena VlatkovicClaudia QuedenauGeorgi TushevIrina EpsteinBernhard SchaefkeWei SunLiang FangGuipeng LiYuhui HuErin M. SchumanWei ChenNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-15 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Xi Wang Xintian You Julian D. Langer Jingyi Hou Fiona Rupprecht Irena Vlatkovic Claudia Quedenau Georgi Tushev Irina Epstein Bernhard Schaefke Wei Sun Liang Fang Guipeng Li Yuhui Hu Erin M. Schuman Wei Chen Full-length transcriptome reconstruction reveals a large diversity of RNA and protein isoforms in rat hippocampus
description	It is challenging to characterize diverse transcript isoforms by short-read sequencing. Here the authors report full-length transcriptomes in rat hippocampus by hybrid-sequencing, predict isoform-specific translational status, and reconstruct open reading frames validated by mass spectrometry.
format	article
author	Xi Wang Xintian You Julian D. Langer Jingyi Hou Fiona Rupprecht Irena Vlatkovic Claudia Quedenau Georgi Tushev Irina Epstein Bernhard Schaefke Wei Sun Liang Fang Guipeng Li Yuhui Hu Erin M. Schuman Wei Chen
author_facet	Xi Wang Xintian You Julian D. Langer Jingyi Hou Fiona Rupprecht Irena Vlatkovic Claudia Quedenau Georgi Tushev Irina Epstein Bernhard Schaefke Wei Sun Liang Fang Guipeng Li Yuhui Hu Erin M. Schuman Wei Chen
author_sort	Xi Wang
title	Full-length transcriptome reconstruction reveals a large diversity of RNA and protein isoforms in rat hippocampus
title_short	Full-length transcriptome reconstruction reveals a large diversity of RNA and protein isoforms in rat hippocampus
title_full	Full-length transcriptome reconstruction reveals a large diversity of RNA and protein isoforms in rat hippocampus
title_fullStr	Full-length transcriptome reconstruction reveals a large diversity of RNA and protein isoforms in rat hippocampus
title_full_unstemmed	Full-length transcriptome reconstruction reveals a large diversity of RNA and protein isoforms in rat hippocampus
title_sort	full-length transcriptome reconstruction reveals a large diversity of rna and protein isoforms in rat hippocampus
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/e648ad9d85e9487fb44af5a9caede55c
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Full-length transcriptome reconstruction reveals a large diversity of RNA and protein isoforms in rat hippocampus

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