A divergent Anaplasma phagocytophilum variant in an Ixodes tick from a migratory bird; Mediterranean basin

Anaplasma phagocytophilum (AP) has vast geographical and host ranges and causes disease in humans and domesticated animals. We investigated the role of northward migratory birds in the dispersal of tick-borne AP in the African-Western Palearctic. Ticks were collected from northward migratory birds t...

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Autores principales:	Tove Hoffman, Peter Wilhelmsson, Christos Barboutis, Thord Fransson, Thomas G.T. Jaenson, Per-Eric Lindgren, Friederike D. Von Loewenich, Åke Lundkvist, Björn Olsen, Erik Salaneck
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	bird migration african-western palearctic region hyalomma marginatum s.l. ixodes anaplasma phagocytophilum 16s rrna anka Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/e6509f73a4b64515b564d5274cd2d519
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spelling	oai:doaj.org-article:e6509f73a4b64515b564d5274cd2d5192021-11-04T15:00:43ZA divergent Anaplasma phagocytophilum variant in an Ixodes tick from a migratory bird; Mediterranean basin2000-868610.1080/20008686.2020.1729653https://doaj.org/article/e6509f73a4b64515b564d5274cd2d5192020-01-01T00:00:00Zhttp://dx.doi.org/10.1080/20008686.2020.1729653https://doaj.org/toc/2000-8686Anaplasma phagocytophilum (AP) has vast geographical and host ranges and causes disease in humans and domesticated animals. We investigated the role of northward migratory birds in the dispersal of tick-borne AP in the African-Western Palearctic. Ticks were collected from northward migratory birds trapped during spring migration of 2010 at two localities in the central Mediterranean Sea. AP DNA was detected by PCR (gltA and 16S rRNA) and variant determination was performed using ankA sequences. In total, 358 ticks were collected. One of 19 ticks determined as Ixodes was confirmed positive for AP DNA. The tick was collected from a woodchat shrike (Lanius senator senator) trapped in Greece, and molecularly determined to belong to the I. ricinus complex and sharing highest (95%) 16S RNA sequence identity to I. gibbosus. The ankA AP sequence exhibited highest similarity to sequences from rodents and shrews (82%) and ruminants (80%). Phylogenetic analyses placed it convincingly outside other clades, suggesting that it represents a novel AP variant. The divergent Ixodes species harboring a novel AP variant could either indicate an enzootic cycle involving co-evolution with birds, or dissemination from other regions by avian migration. None of the 331 Hyalomma marginatum sensu lato ticks, all immature stages, were positive for AP DNA, lending no evidence for the involvement of Hyalomma ticks transported by birds in the ecology of AP.Tove HoffmanPeter WilhelmssonChristos BarboutisThord FranssonThomas G.T. JaensonPer-Eric LindgrenFriederike D. Von LoewenichÅke LundkvistBjörn OlsenErik SalaneckTaylor & Francis Grouparticlebird migrationafrican-western palearctic regionhyalomma marginatum s.l.ixodesanaplasma phagocytophilum16s rrnaankaInfectious and parasitic diseasesRC109-216ENInfection Ecology & Epidemiology, Vol 10, Iss 1 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	bird migration african-western palearctic region hyalomma marginatum s.l. ixodes anaplasma phagocytophilum 16s rrna anka Infectious and parasitic diseases RC109-216
spellingShingle	bird migration african-western palearctic region hyalomma marginatum s.l. ixodes anaplasma phagocytophilum 16s rrna anka Infectious and parasitic diseases RC109-216 Tove Hoffman Peter Wilhelmsson Christos Barboutis Thord Fransson Thomas G.T. Jaenson Per-Eric Lindgren Friederike D. Von Loewenich Åke Lundkvist Björn Olsen Erik Salaneck A divergent Anaplasma phagocytophilum variant in an Ixodes tick from a migratory bird; Mediterranean basin
description	Anaplasma phagocytophilum (AP) has vast geographical and host ranges and causes disease in humans and domesticated animals. We investigated the role of northward migratory birds in the dispersal of tick-borne AP in the African-Western Palearctic. Ticks were collected from northward migratory birds trapped during spring migration of 2010 at two localities in the central Mediterranean Sea. AP DNA was detected by PCR (gltA and 16S rRNA) and variant determination was performed using ankA sequences. In total, 358 ticks were collected. One of 19 ticks determined as Ixodes was confirmed positive for AP DNA. The tick was collected from a woodchat shrike (Lanius senator senator) trapped in Greece, and molecularly determined to belong to the I. ricinus complex and sharing highest (95%) 16S RNA sequence identity to I. gibbosus. The ankA AP sequence exhibited highest similarity to sequences from rodents and shrews (82%) and ruminants (80%). Phylogenetic analyses placed it convincingly outside other clades, suggesting that it represents a novel AP variant. The divergent Ixodes species harboring a novel AP variant could either indicate an enzootic cycle involving co-evolution with birds, or dissemination from other regions by avian migration. None of the 331 Hyalomma marginatum sensu lato ticks, all immature stages, were positive for AP DNA, lending no evidence for the involvement of Hyalomma ticks transported by birds in the ecology of AP.
format	article
author	Tove Hoffman Peter Wilhelmsson Christos Barboutis Thord Fransson Thomas G.T. Jaenson Per-Eric Lindgren Friederike D. Von Loewenich Åke Lundkvist Björn Olsen Erik Salaneck
author_facet	Tove Hoffman Peter Wilhelmsson Christos Barboutis Thord Fransson Thomas G.T. Jaenson Per-Eric Lindgren Friederike D. Von Loewenich Åke Lundkvist Björn Olsen Erik Salaneck
author_sort	Tove Hoffman
title	A divergent Anaplasma phagocytophilum variant in an Ixodes tick from a migratory bird; Mediterranean basin
title_short	A divergent Anaplasma phagocytophilum variant in an Ixodes tick from a migratory bird; Mediterranean basin
title_full	A divergent Anaplasma phagocytophilum variant in an Ixodes tick from a migratory bird; Mediterranean basin
title_fullStr	A divergent Anaplasma phagocytophilum variant in an Ixodes tick from a migratory bird; Mediterranean basin
title_full_unstemmed	A divergent Anaplasma phagocytophilum variant in an Ixodes tick from a migratory bird; Mediterranean basin
title_sort	divergent anaplasma phagocytophilum variant in an ixodes tick from a migratory bird; mediterranean basin
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/e6509f73a4b64515b564d5274cd2d519
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A divergent Anaplasma phagocytophilum variant in an Ixodes tick from a migratory bird; Mediterranean basin

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