Clostridium perfringens α-toxin impairs erythropoiesis by inhibition of erythroid differentiation

Abstract Clostridium perfringens α-toxin induces hemolysis of erythrocytes from various species, but it has not been elucidated whether the toxin affects erythropoiesis. In this study, we treated bone marrow cells (BMCs) from mice with purified α-toxin and found that TER119+ erythroblasts were great...

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Autores principales:	Teruhisa Takagishi, Masaya Takehara, Soshi Seike, Kazuaki Miyamoto, Keiko Kobayashi, Masahiro Nagahama
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e6573dc0560148879093d39d2ad95eea
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spelling	oai:doaj.org-article:e6573dc0560148879093d39d2ad95eea2021-12-02T11:52:37ZClostridium perfringens α-toxin impairs erythropoiesis by inhibition of erythroid differentiation10.1038/s41598-017-05567-82045-2322https://doaj.org/article/e6573dc0560148879093d39d2ad95eea2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05567-8https://doaj.org/toc/2045-2322Abstract Clostridium perfringens α-toxin induces hemolysis of erythrocytes from various species, but it has not been elucidated whether the toxin affects erythropoiesis. In this study, we treated bone marrow cells (BMCs) from mice with purified α-toxin and found that TER119+ erythroblasts were greatly decreased by the treatment. A variant α-toxin defective in enzymatic activities, phospholipase C and sphingomyelinase, had no effect on the population of erythroblasts, demonstrating that the decrease in erythroblasts was dependent of its enzymatic activities. α-Toxin reduced the CD71+TER119+ and CD71–TER119+ cell populations but not the CD71+TER119− cell population. In addition, α-toxin decreased the number of colony-forming unit erythroid colonies but not burst-forming unit erythroid colonies, indicating that α-toxin preferentially reduced mature erythroid cells compared with immature cells. α-Toxin slightly increased annexinV+ cells in TER119+ cells. Additionally, simultaneous treatment of BMCs with α-toxin and erythropoietin greatly attenuated the reduction of TER119+ erythroblasts by α-toxin. Furthermore, hemin-induced differentiation of human K562 erythroleukemia cells was impaired by α-toxin, whereas the treatment exhibited no apparent cytotoxicity. These results suggested that α-toxin mainly inhibited erythroid differentiation. Together, our results provide new insights into the biological activities of α-toxin, which might be important to understand the pathogenesis of C. perfringens infection.Teruhisa TakagishiMasaya TakeharaSoshi SeikeKazuaki MiyamotoKeiko KobayashiMasahiro NagahamaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Teruhisa Takagishi Masaya Takehara Soshi Seike Kazuaki Miyamoto Keiko Kobayashi Masahiro Nagahama Clostridium perfringens α-toxin impairs erythropoiesis by inhibition of erythroid differentiation
description	Abstract Clostridium perfringens α-toxin induces hemolysis of erythrocytes from various species, but it has not been elucidated whether the toxin affects erythropoiesis. In this study, we treated bone marrow cells (BMCs) from mice with purified α-toxin and found that TER119+ erythroblasts were greatly decreased by the treatment. A variant α-toxin defective in enzymatic activities, phospholipase C and sphingomyelinase, had no effect on the population of erythroblasts, demonstrating that the decrease in erythroblasts was dependent of its enzymatic activities. α-Toxin reduced the CD71+TER119+ and CD71–TER119+ cell populations but not the CD71+TER119− cell population. In addition, α-toxin decreased the number of colony-forming unit erythroid colonies but not burst-forming unit erythroid colonies, indicating that α-toxin preferentially reduced mature erythroid cells compared with immature cells. α-Toxin slightly increased annexinV+ cells in TER119+ cells. Additionally, simultaneous treatment of BMCs with α-toxin and erythropoietin greatly attenuated the reduction of TER119+ erythroblasts by α-toxin. Furthermore, hemin-induced differentiation of human K562 erythroleukemia cells was impaired by α-toxin, whereas the treatment exhibited no apparent cytotoxicity. These results suggested that α-toxin mainly inhibited erythroid differentiation. Together, our results provide new insights into the biological activities of α-toxin, which might be important to understand the pathogenesis of C. perfringens infection.
format	article
author	Teruhisa Takagishi Masaya Takehara Soshi Seike Kazuaki Miyamoto Keiko Kobayashi Masahiro Nagahama
author_facet	Teruhisa Takagishi Masaya Takehara Soshi Seike Kazuaki Miyamoto Keiko Kobayashi Masahiro Nagahama
author_sort	Teruhisa Takagishi
title	Clostridium perfringens α-toxin impairs erythropoiesis by inhibition of erythroid differentiation
title_short	Clostridium perfringens α-toxin impairs erythropoiesis by inhibition of erythroid differentiation
title_full	Clostridium perfringens α-toxin impairs erythropoiesis by inhibition of erythroid differentiation
title_fullStr	Clostridium perfringens α-toxin impairs erythropoiesis by inhibition of erythroid differentiation
title_full_unstemmed	Clostridium perfringens α-toxin impairs erythropoiesis by inhibition of erythroid differentiation
title_sort	clostridium perfringens α-toxin impairs erythropoiesis by inhibition of erythroid differentiation
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/e6573dc0560148879093d39d2ad95eea
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Clostridium perfringens α-toxin impairs erythropoiesis by inhibition of erythroid differentiation

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