Fabrication and characterization of scaffolds containing different amounts of allantoin for skin tissue engineering

Abstract Using the skin tissue engineering approach is a way to help the body to recover its lost skin in cases that the spontaneous healing process is either impossible or inadequate, such as severe wounds or burns. In the present study, chitosan/gelatin-based scaffolds containing 0.25, 0.5, 0.75,...

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Autores principales:	Yeganeh Dorri Nokoorani, Amir Shamloo, Maedeh Bahadoran, Hamideh Moravvej
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e6614b6561894cdc84d75d69a0ce6343
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spelling	oai:doaj.org-article:e6614b6561894cdc84d75d69a0ce63432021-12-02T15:08:38ZFabrication and characterization of scaffolds containing different amounts of allantoin for skin tissue engineering10.1038/s41598-021-95763-42045-2322https://doaj.org/article/e6614b6561894cdc84d75d69a0ce63432021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95763-4https://doaj.org/toc/2045-2322Abstract Using the skin tissue engineering approach is a way to help the body to recover its lost skin in cases that the spontaneous healing process is either impossible or inadequate, such as severe wounds or burns. In the present study, chitosan/gelatin-based scaffolds containing 0.25, 0.5, 0.75, and 1% allantoin were created to improve the wounds’ healing process. EDC and NHS were used to cross-link the samples, which were further freeze-dried. Different in-vitro methods were utilized to characterize the specimens, including SEM imaging, PBS absorption and degradation tests, mechanical experiments, allantoin release profile assessment, antibacterial assay, and cell viability and adhesion tests. The results indicated that the scaffolds’ average pore sizes were approximately in the range of 390–440 µm, and their PBS uptake amounts were about 1000% to 1250% after being soaked in PBS for 24 h. Around 70% of the specimens were degraded in 6 days, but they were not fully degraded after 21 days. Besides, the samples showed antibacterial activity against S. aureus and E. coli bacteria. In general, the MTT cell viability test indicated that the cells’ density increased slightly or remained the same during the experiment. SEM images of cells seeded on the scaffolds indicated appropriate properties of the scaffolds for cell adhesion.Yeganeh Dorri NokooraniAmir ShamlooMaedeh BahadoranHamideh MoravvejNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-20 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yeganeh Dorri Nokoorani Amir Shamloo Maedeh Bahadoran Hamideh Moravvej Fabrication and characterization of scaffolds containing different amounts of allantoin for skin tissue engineering
description	Abstract Using the skin tissue engineering approach is a way to help the body to recover its lost skin in cases that the spontaneous healing process is either impossible or inadequate, such as severe wounds or burns. In the present study, chitosan/gelatin-based scaffolds containing 0.25, 0.5, 0.75, and 1% allantoin were created to improve the wounds’ healing process. EDC and NHS were used to cross-link the samples, which were further freeze-dried. Different in-vitro methods were utilized to characterize the specimens, including SEM imaging, PBS absorption and degradation tests, mechanical experiments, allantoin release profile assessment, antibacterial assay, and cell viability and adhesion tests. The results indicated that the scaffolds’ average pore sizes were approximately in the range of 390–440 µm, and their PBS uptake amounts were about 1000% to 1250% after being soaked in PBS for 24 h. Around 70% of the specimens were degraded in 6 days, but they were not fully degraded after 21 days. Besides, the samples showed antibacterial activity against S. aureus and E. coli bacteria. In general, the MTT cell viability test indicated that the cells’ density increased slightly or remained the same during the experiment. SEM images of cells seeded on the scaffolds indicated appropriate properties of the scaffolds for cell adhesion.
format	article
author	Yeganeh Dorri Nokoorani Amir Shamloo Maedeh Bahadoran Hamideh Moravvej
author_facet	Yeganeh Dorri Nokoorani Amir Shamloo Maedeh Bahadoran Hamideh Moravvej
author_sort	Yeganeh Dorri Nokoorani
title	Fabrication and characterization of scaffolds containing different amounts of allantoin for skin tissue engineering
title_short	Fabrication and characterization of scaffolds containing different amounts of allantoin for skin tissue engineering
title_full	Fabrication and characterization of scaffolds containing different amounts of allantoin for skin tissue engineering
title_fullStr	Fabrication and characterization of scaffolds containing different amounts of allantoin for skin tissue engineering
title_full_unstemmed	Fabrication and characterization of scaffolds containing different amounts of allantoin for skin tissue engineering
title_sort	fabrication and characterization of scaffolds containing different amounts of allantoin for skin tissue engineering
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e6614b6561894cdc84d75d69a0ce6343
work_keys_str_mv	AT yeganehdorrinokoorani fabricationandcharacterizationofscaffoldscontainingdifferentamountsofallantoinforskintissueengineering AT amirshamloo fabricationandcharacterizationofscaffoldscontainingdifferentamountsofallantoinforskintissueengineering AT maedehbahadoran fabricationandcharacterizationofscaffoldscontainingdifferentamountsofallantoinforskintissueengineering AT hamidehmoravvej fabricationandcharacterizationofscaffoldscontainingdifferentamountsofallantoinforskintissueengineering
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Fabrication and characterization of scaffolds containing different amounts of allantoin for skin tissue engineering

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