Conditioned taste aversion memory extinction temporally induces insular cortical BDNF release and inhibits neuronal apoptosis

Dian-Wei Liu,1,* Ling Ma,2,* Xu-Hua Zhang,2 Yun-Yan Wang31Department of Neurosurgery, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong 250013, People’s Republic of China; 2Department of Clinical Laboratory, The Second Hospital of Shandong University, Jinan, Shandon...

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Autores principales:	Liu DW, Ma L, Zhang XH, Wang YY
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	brain derived neurotrophic factor (BDNF) insular cortex (IC) conditioned taste aversion (CTA) secretion apoptosis Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/e666c7eea24f4b919248c924a1ce0ee8
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spelling	oai:doaj.org-article:e666c7eea24f4b919248c924a1ce0ee82021-12-02T07:56:47ZConditioned taste aversion memory extinction temporally induces insular cortical BDNF release and inhibits neuronal apoptosis1178-2021https://doaj.org/article/e666c7eea24f4b919248c924a1ce0ee82019-08-01T00:00:00Zhttps://www.dovepress.com/conditioned-taste-aversion-memory-extinction-temporally-induces-insula-peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021Dian-Wei Liu,1,* Ling Ma,2,* Xu-Hua Zhang,2 Yun-Yan Wang31Department of Neurosurgery, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong 250013, People’s Republic of China; 2Department of Clinical Laboratory, The Second Hospital of Shandong University, Jinan, Shandong 250033, People’s Republic of China; 3Department of Neurosurgery, QiLu Hospital of Shandong University, Jinan, Shandong 250012, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yun-Yan WangDepartment of Neurosurgery, QiLu Hospital of Shandong University, No. 44 Wen Hua Xi Road, Jinan, Shandong 250012, People’s Republic of ChinaTel +86 5 311 370 892 8040Fax +86 5 318 569 5463Email wyyqlh123@163.comBackground: Memory extinction has been reported to be related to psychiatric disorders, such as post-traumatic stress disorder (PTSD). Secretion and synthesis of brain-derived neurotrophic factor (BDNF) have been shown to temporally regulate various memory processes via activation of tropomyosin-related kinase B (TrkB) receptors. However, whether memory extinction induces the synthesis and secretion of BDNF on the basis of its localization is not understood. In this study, we aim to investigate activity-dependent BDNF secretion and synthesis in the insular cortex (IC) in the setting of conditioned taste aversion (CTA) memory extinction.Materials and methods: Rats were subjected to CTA memory extinction and BDNF antibody (or the equal volume of vehicle) was microinjected into the IC immediately after the extinction testing. Real-time polymerase chain reaction and in situ hybridization were used to detect the gene expression of BDNF, NGF and NT4. The protein levels of BDNF were determined through the enzyme-linked immunosorbent assay. In addition, the levels of phosphorylated TrkB normalized to total TrkB were evaluated using immunoprecipitation and immunoblotting. c-Fos, total extracellular signal-regulated kinase (Erk), phosphorylated Erk, and apoptosis-related protein (caspase-3), were detected by Western blotting.Results: We found that blocking BDNF signaling within the IC disrupts CTA extinction, suggesting that BDNF signaling in the IC is necessary for CTA extinction. Increased expression levels of c-Fos indicate the induced neuronal activity in the IC during CTA extinction. In addition, temporal changes in the gene expression and protein levels of BDNF in the IC were noted during extinction. Moreover, we found that phosphorylation of TrkB increased prior to the enhanced BDNF expression, suggesting that CTA extinction induces rapid activity-dependent BDNF secretion in the IC. Finally, we found decreased expression of caspase-3 in the IC after CTA extinction.Conclusion: These results demonstrate that CTA memory extinction temporally induces the release and synthesis of BDNF in the IC and inhibits neuronal apoptosis.Keywords: brain derived neurotrophic factor, insular cortex, conditioned taste aversion, secretion, apoptosisLiu DWMa LZhang XHWang YYDove Medical Pressarticlebrain derived neurotrophic factor (BDNF)insular cortex (IC)conditioned taste aversion (CTA)secretionapoptosisNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 15, Pp 2403-2414 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	brain derived neurotrophic factor (BDNF) insular cortex (IC) conditioned taste aversion (CTA) secretion apoptosis Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
spellingShingle	brain derived neurotrophic factor (BDNF) insular cortex (IC) conditioned taste aversion (CTA) secretion apoptosis Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Liu DW Ma L Zhang XH Wang YY Conditioned taste aversion memory extinction temporally induces insular cortical BDNF release and inhibits neuronal apoptosis
description	Dian-Wei Liu,1,* Ling Ma,2,* Xu-Hua Zhang,2 Yun-Yan Wang31Department of Neurosurgery, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong 250013, People’s Republic of China; 2Department of Clinical Laboratory, The Second Hospital of Shandong University, Jinan, Shandong 250033, People’s Republic of China; 3Department of Neurosurgery, QiLu Hospital of Shandong University, Jinan, Shandong 250012, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yun-Yan WangDepartment of Neurosurgery, QiLu Hospital of Shandong University, No. 44 Wen Hua Xi Road, Jinan, Shandong 250012, People’s Republic of ChinaTel +86 5 311 370 892 8040Fax +86 5 318 569 5463Email wyyqlh123@163.comBackground: Memory extinction has been reported to be related to psychiatric disorders, such as post-traumatic stress disorder (PTSD). Secretion and synthesis of brain-derived neurotrophic factor (BDNF) have been shown to temporally regulate various memory processes via activation of tropomyosin-related kinase B (TrkB) receptors. However, whether memory extinction induces the synthesis and secretion of BDNF on the basis of its localization is not understood. In this study, we aim to investigate activity-dependent BDNF secretion and synthesis in the insular cortex (IC) in the setting of conditioned taste aversion (CTA) memory extinction.Materials and methods: Rats were subjected to CTA memory extinction and BDNF antibody (or the equal volume of vehicle) was microinjected into the IC immediately after the extinction testing. Real-time polymerase chain reaction and in situ hybridization were used to detect the gene expression of BDNF, NGF and NT4. The protein levels of BDNF were determined through the enzyme-linked immunosorbent assay. In addition, the levels of phosphorylated TrkB normalized to total TrkB were evaluated using immunoprecipitation and immunoblotting. c-Fos, total extracellular signal-regulated kinase (Erk), phosphorylated Erk, and apoptosis-related protein (caspase-3), were detected by Western blotting.Results: We found that blocking BDNF signaling within the IC disrupts CTA extinction, suggesting that BDNF signaling in the IC is necessary for CTA extinction. Increased expression levels of c-Fos indicate the induced neuronal activity in the IC during CTA extinction. In addition, temporal changes in the gene expression and protein levels of BDNF in the IC were noted during extinction. Moreover, we found that phosphorylation of TrkB increased prior to the enhanced BDNF expression, suggesting that CTA extinction induces rapid activity-dependent BDNF secretion in the IC. Finally, we found decreased expression of caspase-3 in the IC after CTA extinction.Conclusion: These results demonstrate that CTA memory extinction temporally induces the release and synthesis of BDNF in the IC and inhibits neuronal apoptosis.Keywords: brain derived neurotrophic factor, insular cortex, conditioned taste aversion, secretion, apoptosis
format	article
author	Liu DW Ma L Zhang XH Wang YY
author_facet	Liu DW Ma L Zhang XH Wang YY
author_sort	Liu DW
title	Conditioned taste aversion memory extinction temporally induces insular cortical BDNF release and inhibits neuronal apoptosis
title_short	Conditioned taste aversion memory extinction temporally induces insular cortical BDNF release and inhibits neuronal apoptosis
title_full	Conditioned taste aversion memory extinction temporally induces insular cortical BDNF release and inhibits neuronal apoptosis
title_fullStr	Conditioned taste aversion memory extinction temporally induces insular cortical BDNF release and inhibits neuronal apoptosis
title_full_unstemmed	Conditioned taste aversion memory extinction temporally induces insular cortical BDNF release and inhibits neuronal apoptosis
title_sort	conditioned taste aversion memory extinction temporally induces insular cortical bdnf release and inhibits neuronal apoptosis
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/e666c7eea24f4b919248c924a1ce0ee8
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_version_	1718399134582439936

Conditioned taste aversion memory extinction temporally induces insular cortical BDNF release and inhibits neuronal apoptosis

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