Differential and Common Signatures of miRNA Expression and Methylation in Childhood Central Nervous System Malignancies: An Experimental and Computational Approach

Epigenetic modifications are considered of utmost significance for tumor ontogenesis and progression. Especially, it has been found that miRNA expression, as well as DNA methylation plays a significant role in central nervous system tumors during childhood. A total of 49 resected brain tumors from c...

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Autores principales:	George I. Lambrou, Myrto Poulou, Krinio Giannikou, Marios Themistocleous, Apostolos Zaravinos, Maria Braoudaki
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	methylation childhood CNS tumors miRNA mRNA microarray Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/e6699fccef764898a623cf366ab6f6bb
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spelling	oai:doaj.org-article:e6699fccef764898a623cf366ab6f6bb2021-11-11T15:33:42ZDifferential and Common Signatures of miRNA Expression and Methylation in Childhood Central Nervous System Malignancies: An Experimental and Computational Approach10.3390/cancers132154912072-6694https://doaj.org/article/e6699fccef764898a623cf366ab6f6bb2021-10-01T00:00:00Zhttps://www.mdpi.com/2072-6694/13/21/5491https://doaj.org/toc/2072-6694Epigenetic modifications are considered of utmost significance for tumor ontogenesis and progression. Especially, it has been found that miRNA expression, as well as DNA methylation plays a significant role in central nervous system tumors during childhood. A total of 49 resected brain tumors from children were used for further analysis. DNA methylation was identified with methylation-specific MLPA and, in particular, for the tumor suppressor genes CASP8, RASSF1, MGMT, MSH6, GATA5, ATM1, TP53, and CADM1. miRNAs were identified with microarray screening, as well as selected samples, were tested for their mRNA expression levels. CASP8, RASSF1 were the most frequently methylated genes in all tumor samples. Simultaneous methylation of genes manifested significant results with respect to tumor staging, tumor type, and the differentiation of tumor and control samples. There was no significant dependence observed with the methylation of one gene promoter, rather with the simultaneous presence of all detected methylated genes’ promoters. miRNA expression was found to be correlated to gene methylation. Epigenetic regulation appears to be of major importance in tumor progression and pathophysiology, making it an imperative field of study.George I. LambrouMyrto PoulouKrinio GiannikouMarios ThemistocleousApostolos ZaravinosMaria BraoudakiMDPI AGarticlemethylationchildhood CNS tumorsmiRNAmRNAmicroarrayNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENCancers, Vol 13, Iss 5491, p 5491 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	methylation childhood CNS tumors miRNA mRNA microarray Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	methylation childhood CNS tumors miRNA mRNA microarray Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 George I. Lambrou Myrto Poulou Krinio Giannikou Marios Themistocleous Apostolos Zaravinos Maria Braoudaki Differential and Common Signatures of miRNA Expression and Methylation in Childhood Central Nervous System Malignancies: An Experimental and Computational Approach
description	Epigenetic modifications are considered of utmost significance for tumor ontogenesis and progression. Especially, it has been found that miRNA expression, as well as DNA methylation plays a significant role in central nervous system tumors during childhood. A total of 49 resected brain tumors from children were used for further analysis. DNA methylation was identified with methylation-specific MLPA and, in particular, for the tumor suppressor genes CASP8, RASSF1, MGMT, MSH6, GATA5, ATM1, TP53, and CADM1. miRNAs were identified with microarray screening, as well as selected samples, were tested for their mRNA expression levels. CASP8, RASSF1 were the most frequently methylated genes in all tumor samples. Simultaneous methylation of genes manifested significant results with respect to tumor staging, tumor type, and the differentiation of tumor and control samples. There was no significant dependence observed with the methylation of one gene promoter, rather with the simultaneous presence of all detected methylated genes’ promoters. miRNA expression was found to be correlated to gene methylation. Epigenetic regulation appears to be of major importance in tumor progression and pathophysiology, making it an imperative field of study.
format	article
author	George I. Lambrou Myrto Poulou Krinio Giannikou Marios Themistocleous Apostolos Zaravinos Maria Braoudaki
author_facet	George I. Lambrou Myrto Poulou Krinio Giannikou Marios Themistocleous Apostolos Zaravinos Maria Braoudaki
author_sort	George I. Lambrou
title	Differential and Common Signatures of miRNA Expression and Methylation in Childhood Central Nervous System Malignancies: An Experimental and Computational Approach
title_short	Differential and Common Signatures of miRNA Expression and Methylation in Childhood Central Nervous System Malignancies: An Experimental and Computational Approach
title_full	Differential and Common Signatures of miRNA Expression and Methylation in Childhood Central Nervous System Malignancies: An Experimental and Computational Approach
title_fullStr	Differential and Common Signatures of miRNA Expression and Methylation in Childhood Central Nervous System Malignancies: An Experimental and Computational Approach
title_full_unstemmed	Differential and Common Signatures of miRNA Expression and Methylation in Childhood Central Nervous System Malignancies: An Experimental and Computational Approach
title_sort	differential and common signatures of mirna expression and methylation in childhood central nervous system malignancies: an experimental and computational approach
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e6699fccef764898a623cf366ab6f6bb
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Differential and Common Signatures of miRNA Expression and Methylation in Childhood Central Nervous System Malignancies: An Experimental and Computational Approach

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