Genomic regions and genes related to inter-population differences in body size in the ground beetle Carabus japonicus

Abstract Body size is a key trait in diversification among animal species, and revealing the gene regions responsible for body size diversification among populations or related species is important in evolutionary biology. We explored the genomic regions associated with body size differences in Cara...

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Autores principales:	Ryohei Komurai, Tomochika Fujisawa, Yutaka Okuzaki, Teiji Sota
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e66c44bf75d0462798da17942eef26c7
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spelling	oai:doaj.org-article:e66c44bf75d0462798da17942eef26c72021-12-02T12:32:20ZGenomic regions and genes related to inter-population differences in body size in the ground beetle Carabus japonicus10.1038/s41598-017-08362-72045-2322https://doaj.org/article/e66c44bf75d0462798da17942eef26c72017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08362-7https://doaj.org/toc/2045-2322Abstract Body size is a key trait in diversification among animal species, and revealing the gene regions responsible for body size diversification among populations or related species is important in evolutionary biology. We explored the genomic regions associated with body size differences in Carabus japonicus ground beetle populations by quantitative trait locus (QTL) mapping of F2 hybrids from differently sized parents from two populations using restriction site-associated DNA sequencing and de novo assembly of the beetle whole genome. The assembled genome had a total length of 191 Mb with a scaffold N50 of 0.73 Mb; 14,929 protein-coding genes were predicted. Three QTLs on different linkage groups had major effects on the overall size, which is composed chiefly of elytral length. In addition, we found QTLs on autosomal and X chromosomal linkage groups that affected head length and width, thoracic width, and elytral width. We determined the gene loci potentially related to control of body size in scaffolds of the genome sequence, which contained the QTL regions. The genetic basis of body size variation based on a small number of major loci would promote differentiation in body size in response to selection pressures related to variations in environmental conditions and inter-specific interactions.Ryohei KomuraiTomochika FujisawaYutaka OkuzakiTeiji SotaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ryohei Komurai Tomochika Fujisawa Yutaka Okuzaki Teiji Sota Genomic regions and genes related to inter-population differences in body size in the ground beetle Carabus japonicus
description	Abstract Body size is a key trait in diversification among animal species, and revealing the gene regions responsible for body size diversification among populations or related species is important in evolutionary biology. We explored the genomic regions associated with body size differences in Carabus japonicus ground beetle populations by quantitative trait locus (QTL) mapping of F2 hybrids from differently sized parents from two populations using restriction site-associated DNA sequencing and de novo assembly of the beetle whole genome. The assembled genome had a total length of 191 Mb with a scaffold N50 of 0.73 Mb; 14,929 protein-coding genes were predicted. Three QTLs on different linkage groups had major effects on the overall size, which is composed chiefly of elytral length. In addition, we found QTLs on autosomal and X chromosomal linkage groups that affected head length and width, thoracic width, and elytral width. We determined the gene loci potentially related to control of body size in scaffolds of the genome sequence, which contained the QTL regions. The genetic basis of body size variation based on a small number of major loci would promote differentiation in body size in response to selection pressures related to variations in environmental conditions and inter-specific interactions.
format	article
author	Ryohei Komurai Tomochika Fujisawa Yutaka Okuzaki Teiji Sota
author_facet	Ryohei Komurai Tomochika Fujisawa Yutaka Okuzaki Teiji Sota
author_sort	Ryohei Komurai
title	Genomic regions and genes related to inter-population differences in body size in the ground beetle Carabus japonicus
title_short	Genomic regions and genes related to inter-population differences in body size in the ground beetle Carabus japonicus
title_full	Genomic regions and genes related to inter-population differences in body size in the ground beetle Carabus japonicus
title_fullStr	Genomic regions and genes related to inter-population differences in body size in the ground beetle Carabus japonicus
title_full_unstemmed	Genomic regions and genes related to inter-population differences in body size in the ground beetle Carabus japonicus
title_sort	genomic regions and genes related to inter-population differences in body size in the ground beetle carabus japonicus
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/e66c44bf75d0462798da17942eef26c7
work_keys_str_mv	AT ryoheikomurai genomicregionsandgenesrelatedtointerpopulationdifferencesinbodysizeinthegroundbeetlecarabusjaponicus AT tomochikafujisawa genomicregionsandgenesrelatedtointerpopulationdifferencesinbodysizeinthegroundbeetlecarabusjaponicus AT yutakaokuzaki genomicregionsandgenesrelatedtointerpopulationdifferencesinbodysizeinthegroundbeetlecarabusjaponicus AT teijisota genomicregionsandgenesrelatedtointerpopulationdifferencesinbodysizeinthegroundbeetlecarabusjaponicus
_version_	1718394128960585728

Genomic regions and genes related to inter-population differences in body size in the ground beetle Carabus japonicus

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