Genetic Characterization of Plasmid-Borne <italic toggle="yes">bla</italic><sub>OXA-58</sub> in Distinct <named-content content-type="genus-species">Acinetobacter</named-content> Species

ABSTRACT We characterize by whole-plasmid-sequence (WPS) two-plasmid-borne blaOXA-58 obtained from Acinetobacter seifertii (Asp-1069) and A. baumannii (Acb-45063) clinical strains recovered 17 years apart from distinct Brazilian regions. Multilocus sequence type (MLST) analysis showed that the Asp-1...

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Autores principales:	Adriana P. Matos, Rodrigo Cayô, Luiz G. P. Almeida, Ana Paula Streling, Carolina S. Nodari, Willames M. B. S. Martins, Ana Clara Narciso, Rosa M. Silva, Ana T. R. Vasconcelos, Ana C. Gales
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	Acinetobacter calcoaceticus-Acinetobacter baumannii complex Gram-negative bacilli carbapenem resistance carbapenem-hydrolyzing class D β-lactamase nosocomial infection Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/e676f66cc91e4592bc9bbc17f1050fb2
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spelling	oai:doaj.org-article:e676f66cc91e4592bc9bbc17f1050fb22021-11-15T15:27:32ZGenetic Characterization of Plasmid-Borne <italic toggle="yes">bla</italic><sub>OXA-58</sub> in Distinct <named-content content-type="genus-species">Acinetobacter</named-content> Species10.1128/mSphere.00376-192379-5042https://doaj.org/article/e676f66cc91e4592bc9bbc17f1050fb22019-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00376-19https://doaj.org/toc/2379-5042ABSTRACT We characterize by whole-plasmid-sequence (WPS) two-plasmid-borne blaOXA-58 obtained from Acinetobacter seifertii (Asp-1069) and A. baumannii (Acb-45063) clinical strains recovered 17 years apart from distinct Brazilian regions. Multilocus sequence type (MLST) analysis showed that the Asp-1069 and Acb-45063 strains belong to ST551 and ST15/CC15, respectively. WPS analysis demonstrated that blaOXA-58 was located in two distinct plasmids named pAs1069_a (24,672 bp/44 open reading frames [ORFs]) and pAb45063_b (19,808 bp/24 ORFs), which belong to the GR8/GR23 (repAci23) and GR4 (repAci4) incompatibility groups, respectively. The genetic environments surrounding blaOXA-58 revealed that it was flanked by two intact ISAba3 copies on pAb45063_b, which differed from pAs1069_a. In the latter, the upstream ISAba3 copy was truncated by insertion of ISAba825 element. Although Re27-specific recombination sites were found adjacent to ISAba3-blaOXA-58-ISAba3 arrangement on pAb45063_b, such structures were absent on pAs1069_a. The conserved ISAba125-araC1-lysE arrangement was disrupted by TnaphA6 harboring the aminoglycosides resistance gene aphA6 on pAs1069_a, while an IS26-blaTEM-1-aac(3)-IIa-IS26 genetic structure was found upstream from ISAba3-blaOXA-58-ISAba3 on pAb45063_b. Other two plasmids, pAb45063_a (183,767 bp/209 ORFs) and pAs1069_b (13,129 bp/14 ORFs), were also found in the OXA-58-producing Acinetobacter species strains, harboring the strA and strB genes and the sul2 gene, which confer resistance to streptomycin and sulfonamides, respectively. The plasmid-mediated virulence factors corresponding to genes tonB, spl, glmM, ppa, sulP, and map were found in both strains, as well distinct toxin-antitoxin system-encoding genes stbD and relE (pAs1069_a), brnT and brnA (pAb45063_b), and xreE (pAb45063_a). Although infrequently reported in Brazil, plasmid-borne blaOXA-58 showed a complex and diverse genetic backbone that confers stability in different Acinetobacter species that have been isolated from nosocomial settings over time. IMPORTANCE Although the blaOXA-58 gene has been infrequently described in Brazil, contrasting with other bordering South American countries, we verified the maintenance of this resistance determinant over time among carbapenem-resistant Acinetobacter species isolates, not only in nosocomial settings but also in the environment. In addition, to the best of our knowledge, this is the first study to have used WPS analysis to evaluate the genetic surroundings of blaOXA-58 in Brazil. Moreover, the A. seifertii and A. baumannii clinical strains evaluated in this study were recovered 17 years apart in hospitals located in distinct Brazilian geographic regions.Adriana P. MatosRodrigo CayôLuiz G. P. AlmeidaAna Paula StrelingCarolina S. NodariWillames M. B. S. MartinsAna Clara NarcisoRosa M. SilvaAna T. R. VasconcelosAna C. GalesAmerican Society for MicrobiologyarticleAcinetobacter calcoaceticus-Acinetobacter baumannii complexGram-negative bacillicarbapenem resistancecarbapenem-hydrolyzing class D β-lactamasenosocomial infectionMicrobiologyQR1-502ENmSphere, Vol 4, Iss 5 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Acinetobacter calcoaceticus-Acinetobacter baumannii complex Gram-negative bacilli carbapenem resistance carbapenem-hydrolyzing class D β-lactamase nosocomial infection Microbiology QR1-502
spellingShingle	Acinetobacter calcoaceticus-Acinetobacter baumannii complex Gram-negative bacilli carbapenem resistance carbapenem-hydrolyzing class D β-lactamase nosocomial infection Microbiology QR1-502 Adriana P. Matos Rodrigo Cayô Luiz G. P. Almeida Ana Paula Streling Carolina S. Nodari Willames M. B. S. Martins Ana Clara Narciso Rosa M. Silva Ana T. R. Vasconcelos Ana C. Gales Genetic Characterization of Plasmid-Borne <italic toggle="yes">bla</italic><sub>OXA-58</sub> in Distinct <named-content content-type="genus-species">Acinetobacter</named-content> Species
description	ABSTRACT We characterize by whole-plasmid-sequence (WPS) two-plasmid-borne blaOXA-58 obtained from Acinetobacter seifertii (Asp-1069) and A. baumannii (Acb-45063) clinical strains recovered 17 years apart from distinct Brazilian regions. Multilocus sequence type (MLST) analysis showed that the Asp-1069 and Acb-45063 strains belong to ST551 and ST15/CC15, respectively. WPS analysis demonstrated that blaOXA-58 was located in two distinct plasmids named pAs1069_a (24,672 bp/44 open reading frames [ORFs]) and pAb45063_b (19,808 bp/24 ORFs), which belong to the GR8/GR23 (repAci23) and GR4 (repAci4) incompatibility groups, respectively. The genetic environments surrounding blaOXA-58 revealed that it was flanked by two intact ISAba3 copies on pAb45063_b, which differed from pAs1069_a. In the latter, the upstream ISAba3 copy was truncated by insertion of ISAba825 element. Although Re27-specific recombination sites were found adjacent to ISAba3-blaOXA-58-ISAba3 arrangement on pAb45063_b, such structures were absent on pAs1069_a. The conserved ISAba125-araC1-lysE arrangement was disrupted by TnaphA6 harboring the aminoglycosides resistance gene aphA6 on pAs1069_a, while an IS26-blaTEM-1-aac(3)-IIa-IS26 genetic structure was found upstream from ISAba3-blaOXA-58-ISAba3 on pAb45063_b. Other two plasmids, pAb45063_a (183,767 bp/209 ORFs) and pAs1069_b (13,129 bp/14 ORFs), were also found in the OXA-58-producing Acinetobacter species strains, harboring the strA and strB genes and the sul2 gene, which confer resistance to streptomycin and sulfonamides, respectively. The plasmid-mediated virulence factors corresponding to genes tonB, spl, glmM, ppa, sulP, and map were found in both strains, as well distinct toxin-antitoxin system-encoding genes stbD and relE (pAs1069_a), brnT and brnA (pAb45063_b), and xreE (pAb45063_a). Although infrequently reported in Brazil, plasmid-borne blaOXA-58 showed a complex and diverse genetic backbone that confers stability in different Acinetobacter species that have been isolated from nosocomial settings over time. IMPORTANCE Although the blaOXA-58 gene has been infrequently described in Brazil, contrasting with other bordering South American countries, we verified the maintenance of this resistance determinant over time among carbapenem-resistant Acinetobacter species isolates, not only in nosocomial settings but also in the environment. In addition, to the best of our knowledge, this is the first study to have used WPS analysis to evaluate the genetic surroundings of blaOXA-58 in Brazil. Moreover, the A. seifertii and A. baumannii clinical strains evaluated in this study were recovered 17 years apart in hospitals located in distinct Brazilian geographic regions.
format	article
author	Adriana P. Matos Rodrigo Cayô Luiz G. P. Almeida Ana Paula Streling Carolina S. Nodari Willames M. B. S. Martins Ana Clara Narciso Rosa M. Silva Ana T. R. Vasconcelos Ana C. Gales
author_facet	Adriana P. Matos Rodrigo Cayô Luiz G. P. Almeida Ana Paula Streling Carolina S. Nodari Willames M. B. S. Martins Ana Clara Narciso Rosa M. Silva Ana T. R. Vasconcelos Ana C. Gales
author_sort	Adriana P. Matos
title	Genetic Characterization of Plasmid-Borne <italic toggle="yes">bla</italic><sub>OXA-58</sub> in Distinct <named-content content-type="genus-species">Acinetobacter</named-content> Species
title_short	Genetic Characterization of Plasmid-Borne <italic toggle="yes">bla</italic><sub>OXA-58</sub> in Distinct <named-content content-type="genus-species">Acinetobacter</named-content> Species
title_full	Genetic Characterization of Plasmid-Borne <italic toggle="yes">bla</italic><sub>OXA-58</sub> in Distinct <named-content content-type="genus-species">Acinetobacter</named-content> Species
title_fullStr	Genetic Characterization of Plasmid-Borne <italic toggle="yes">bla</italic><sub>OXA-58</sub> in Distinct <named-content content-type="genus-species">Acinetobacter</named-content> Species
title_full_unstemmed	Genetic Characterization of Plasmid-Borne <italic toggle="yes">bla</italic><sub>OXA-58</sub> in Distinct <named-content content-type="genus-species">Acinetobacter</named-content> Species
title_sort	genetic characterization of plasmid-borne <italic toggle="yes">bla</italic><sub>oxa-58</sub> in distinct <named-content content-type="genus-species">acinetobacter</named-content> species
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/e676f66cc91e4592bc9bbc17f1050fb2
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Genetic Characterization of Plasmid-Borne <italic toggle="yes">bla</italic><sub>OXA-58</sub> in Distinct <named-content content-type="genus-species">Acinetobacter</named-content> Species

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