Human neutrophils phagocytose and kill Acinetobacter baumannii and A. pittii

Abstract Acinetobacter baumannii is a common cause of health care associated infections worldwide. A. pittii is an opportunistic pathogen also frequently isolated from Acinetobacter infections other than those from A. baumannii. Knowledge of Acinetobacter virulence factors and their role in pathogen...

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Autores principales:	María Lázaro-Díez, Itziar Chapartegui-González, Santiago Redondo-Salvo, Chike Leigh, David Merino, David San Segundo, Adrián Fernández, Jesús Navas, José Manuel Icardo, Félix Acosta, Alain Ocampo-Sosa, Luis Martínez-Martínez, José Ramos-Vivas
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e6794eab2b434d979eb1571edcbca8dc
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spelling	oai:doaj.org-article:e6794eab2b434d979eb1571edcbca8dc2021-12-02T11:52:57ZHuman neutrophils phagocytose and kill Acinetobacter baumannii and A. pittii10.1038/s41598-017-04870-82045-2322https://doaj.org/article/e6794eab2b434d979eb1571edcbca8dc2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04870-8https://doaj.org/toc/2045-2322Abstract Acinetobacter baumannii is a common cause of health care associated infections worldwide. A. pittii is an opportunistic pathogen also frequently isolated from Acinetobacter infections other than those from A. baumannii. Knowledge of Acinetobacter virulence factors and their role in pathogenesis is scarce. Also, there are no detailed published reports on the interactions between A. pittii and human phagocytic cells. Using confocal laser and scanning electron microscopy, immunofluorescence, and live-cell imaging, our study shows that immediately after bacteria-cell contact, neutrophils rapidly and continuously engulf and kill bacteria during at least 4 hours of infection in vitro. After 3 h of infection, neutrophils start to release neutrophil extracellular traps (NETs) against Acinetobacter. DNA in NETs colocalizes well with human histone H3 and with the specific neutrophil elastase. We have observed that human neutrophils use large filopodia as cellular tentacles to sense local environment but also to detect and retain bacteria during phagocytosis. Furthermore, co-cultivation of neutrophils with human differentiated macrophages before infections shows that human neutrophils, but not macrophages, are key immune cells to control Acinetobacter. Although macrophages were largely activated by both bacterial species, they lack the phagocytic activity demonstrated by neutrophils.María Lázaro-DíezItziar Chapartegui-GonzálezSantiago Redondo-SalvoChike LeighDavid MerinoDavid San SegundoAdrián FernándezJesús NavasJosé Manuel IcardoFélix AcostaAlain Ocampo-SosaLuis Martínez-MartínezJosé Ramos-VivasNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q María Lázaro-Díez Itziar Chapartegui-González Santiago Redondo-Salvo Chike Leigh David Merino David San Segundo Adrián Fernández Jesús Navas José Manuel Icardo Félix Acosta Alain Ocampo-Sosa Luis Martínez-Martínez José Ramos-Vivas Human neutrophils phagocytose and kill Acinetobacter baumannii and A. pittii
description	Abstract Acinetobacter baumannii is a common cause of health care associated infections worldwide. A. pittii is an opportunistic pathogen also frequently isolated from Acinetobacter infections other than those from A. baumannii. Knowledge of Acinetobacter virulence factors and their role in pathogenesis is scarce. Also, there are no detailed published reports on the interactions between A. pittii and human phagocytic cells. Using confocal laser and scanning electron microscopy, immunofluorescence, and live-cell imaging, our study shows that immediately after bacteria-cell contact, neutrophils rapidly and continuously engulf and kill bacteria during at least 4 hours of infection in vitro. After 3 h of infection, neutrophils start to release neutrophil extracellular traps (NETs) against Acinetobacter. DNA in NETs colocalizes well with human histone H3 and with the specific neutrophil elastase. We have observed that human neutrophils use large filopodia as cellular tentacles to sense local environment but also to detect and retain bacteria during phagocytosis. Furthermore, co-cultivation of neutrophils with human differentiated macrophages before infections shows that human neutrophils, but not macrophages, are key immune cells to control Acinetobacter. Although macrophages were largely activated by both bacterial species, they lack the phagocytic activity demonstrated by neutrophils.
format	article
author	María Lázaro-Díez Itziar Chapartegui-González Santiago Redondo-Salvo Chike Leigh David Merino David San Segundo Adrián Fernández Jesús Navas José Manuel Icardo Félix Acosta Alain Ocampo-Sosa Luis Martínez-Martínez José Ramos-Vivas
author_facet	María Lázaro-Díez Itziar Chapartegui-González Santiago Redondo-Salvo Chike Leigh David Merino David San Segundo Adrián Fernández Jesús Navas José Manuel Icardo Félix Acosta Alain Ocampo-Sosa Luis Martínez-Martínez José Ramos-Vivas
author_sort	María Lázaro-Díez
title	Human neutrophils phagocytose and kill Acinetobacter baumannii and A. pittii
title_short	Human neutrophils phagocytose and kill Acinetobacter baumannii and A. pittii
title_full	Human neutrophils phagocytose and kill Acinetobacter baumannii and A. pittii
title_fullStr	Human neutrophils phagocytose and kill Acinetobacter baumannii and A. pittii
title_full_unstemmed	Human neutrophils phagocytose and kill Acinetobacter baumannii and A. pittii
title_sort	human neutrophils phagocytose and kill acinetobacter baumannii and a. pittii
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/e6794eab2b434d979eb1571edcbca8dc
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Human neutrophils phagocytose and kill Acinetobacter baumannii and A. pittii

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