Locus characterization and gene expression of bovine FNDC5: is the myokine irisin relevant in cattle?

The transmembrane protein FNDC5 was recently characterized as precursor of an exercise induced myokine named irisin. Previous studies found a relationship between circulating irisin levels and muscle mass in humans. Consequently, we tested the hypothesis whether FNDC5/irisin is involved in the modul...

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Autores principales:	Katrin Komolka, Elke Albrecht, Lisa Schering, Julia Brenmoehl, Andreas Hoeflich, Steffen Maak
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e680f4df011e4475b32ed53a9fc6df65
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spelling	oai:doaj.org-article:e680f4df011e4475b32ed53a9fc6df652021-11-18T08:34:24ZLocus characterization and gene expression of bovine FNDC5: is the myokine irisin relevant in cattle?1932-620310.1371/journal.pone.0088060https://doaj.org/article/e680f4df011e4475b32ed53a9fc6df652014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24498244/?tool=EBIhttps://doaj.org/toc/1932-6203The transmembrane protein FNDC5 was recently characterized as precursor of an exercise induced myokine named irisin. Previous studies found a relationship between circulating irisin levels and muscle mass in humans. Consequently, we tested the hypothesis whether FNDC5/irisin is involved in the modulation of body composition in cattle. Since information on the bovine FNDC5 locus was scarce, we characterized the gene experimentally as prerequisite for these investigations. We provide here a revised and extended gene model for bovine FNDC5. Although similarly organized like the human and murine loci, a higher variability was observed at transcript level in the bovine locus. FNDC5 mRNA was abundant in bovine skeletal muscle and was detected at lower levels in adipose tissue and liver. There were no expression differences between two groups of bulls highly different in muscularity and adiposity. Full-length FNDC5 protein (25 kDa) was present in bovine skeletal muscle independent of muscularity. Neither FNDC5 nor its putatively secreted peptide irisin were found in circulation of bulls. In contrast, we demonstrated that FNDC5 (25 kDa) and irisin (12 kDa) were present in murine skeletal muscle and that irisin was circulating in murine serum. This indicates fundamental differences in the regulation of FNDC5 and irisin between rodents and cattle.Katrin KomolkaElke AlbrechtLisa ScheringJulia BrenmoehlAndreas HoeflichSteffen MaakPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e88060 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Katrin Komolka Elke Albrecht Lisa Schering Julia Brenmoehl Andreas Hoeflich Steffen Maak Locus characterization and gene expression of bovine FNDC5: is the myokine irisin relevant in cattle?
description	The transmembrane protein FNDC5 was recently characterized as precursor of an exercise induced myokine named irisin. Previous studies found a relationship between circulating irisin levels and muscle mass in humans. Consequently, we tested the hypothesis whether FNDC5/irisin is involved in the modulation of body composition in cattle. Since information on the bovine FNDC5 locus was scarce, we characterized the gene experimentally as prerequisite for these investigations. We provide here a revised and extended gene model for bovine FNDC5. Although similarly organized like the human and murine loci, a higher variability was observed at transcript level in the bovine locus. FNDC5 mRNA was abundant in bovine skeletal muscle and was detected at lower levels in adipose tissue and liver. There were no expression differences between two groups of bulls highly different in muscularity and adiposity. Full-length FNDC5 protein (25 kDa) was present in bovine skeletal muscle independent of muscularity. Neither FNDC5 nor its putatively secreted peptide irisin were found in circulation of bulls. In contrast, we demonstrated that FNDC5 (25 kDa) and irisin (12 kDa) were present in murine skeletal muscle and that irisin was circulating in murine serum. This indicates fundamental differences in the regulation of FNDC5 and irisin between rodents and cattle.
format	article
author	Katrin Komolka Elke Albrecht Lisa Schering Julia Brenmoehl Andreas Hoeflich Steffen Maak
author_facet	Katrin Komolka Elke Albrecht Lisa Schering Julia Brenmoehl Andreas Hoeflich Steffen Maak
author_sort	Katrin Komolka
title	Locus characterization and gene expression of bovine FNDC5: is the myokine irisin relevant in cattle?
title_short	Locus characterization and gene expression of bovine FNDC5: is the myokine irisin relevant in cattle?
title_full	Locus characterization and gene expression of bovine FNDC5: is the myokine irisin relevant in cattle?
title_fullStr	Locus characterization and gene expression of bovine FNDC5: is the myokine irisin relevant in cattle?
title_full_unstemmed	Locus characterization and gene expression of bovine FNDC5: is the myokine irisin relevant in cattle?
title_sort	locus characterization and gene expression of bovine fndc5: is the myokine irisin relevant in cattle?
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/e680f4df011e4475b32ed53a9fc6df65
work_keys_str_mv	AT katrinkomolka locuscharacterizationandgeneexpressionofbovinefndc5isthemyokineirisinrelevantincattle AT elkealbrecht locuscharacterizationandgeneexpressionofbovinefndc5isthemyokineirisinrelevantincattle AT lisaschering locuscharacterizationandgeneexpressionofbovinefndc5isthemyokineirisinrelevantincattle AT juliabrenmoehl locuscharacterizationandgeneexpressionofbovinefndc5isthemyokineirisinrelevantincattle AT andreashoeflich locuscharacterizationandgeneexpressionofbovinefndc5isthemyokineirisinrelevantincattle AT steffenmaak locuscharacterizationandgeneexpressionofbovinefndc5isthemyokineirisinrelevantincattle
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Locus characterization and gene expression of bovine FNDC5: is the myokine irisin relevant in cattle?

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