Seroprevalence of Zika Virus in Wild African Green Monkeys and Baboons

ABSTRACT Zika virus (ZIKV) has recently spread through the Americas and has been associated with a range of health effects, including birth defects in children born to women infected during pregnancy. Although the natural reservoir of ZIKV remains poorly defined, the virus was first identified in a...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Connor R. Buechler, Adam L. Bailey, Andrea M. Weiler, Gabrielle L. Barry, Meghan E. Breitbach, Laurel M. Stewart, Anna J. Jasinska, Nelson B. Freimer, Cristian Apetrei, Jane E. Phillips-Conroy, Clifford J. Jolly, Jeffrey Rogers, Thomas C. Friedrich, David H. O’Connor
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2017
Materias:	false-positive reactions Flavivirus sensitivity and specificity seroepidemiologic studies Zika virus Zika virus infection Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/e68291cb6bea452e96771d33b7c28b5c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e68291cb6bea452e96771d33b7c28b5c
record_format	dspace
spelling	oai:doaj.org-article:e68291cb6bea452e96771d33b7c28b5c2021-11-15T15:21:45ZSeroprevalence of Zika Virus in Wild African Green Monkeys and Baboons10.1128/mSphere.00392-162379-5042https://doaj.org/article/e68291cb6bea452e96771d33b7c28b5c2017-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00392-16https://doaj.org/toc/2379-5042ABSTRACT Zika virus (ZIKV) has recently spread through the Americas and has been associated with a range of health effects, including birth defects in children born to women infected during pregnancy. Although the natural reservoir of ZIKV remains poorly defined, the virus was first identified in a captive “sentinel” macaque monkey in Africa in 1947. However, the virus has not been reported in humans or nonhuman primates (NHPs) in Africa outside Gabon in over a decade. Here, we examine ZIKV infection in 239 wild baboons and African green monkeys from South Africa, the Gambia, Tanzania, and Zambia using combinations of unbiased deep sequencing, quantitative reverse transcription-PCR (qRT-PCR), and an antibody capture assay that we optimized using serum collected from captive macaque monkeys exposed to ZIKV, dengue virus, and yellow fever virus. While we did not find evidence of active ZIKV infection in wild NHPs in Africa, we found variable ZIKV seropositivity of up to 16% in some of the NHP populations sampled. We anticipate that these results and the methodology described within will help in continued efforts to determine the prevalence, natural reservoir, and transmission dynamics of ZIKV in Africa and elsewhere. IMPORTANCE Zika virus (ZIKV) is a mosquito-borne virus originally discovered in a captive monkey living in the Zika Forest of Uganda, Africa, in 1947. Recently, an outbreak in South America has shown that ZIKV infection can cause myriad health effects, including birth defects in the children of women infected during pregnancy. Here, we sought to investigate ZIKV infection in wild African primates to better understand its emergence and spread, looking for evidence of active or prior infection. Our results suggest that up to 16% of some populations of nonhuman primate were, at some point, exposed to ZIKV. We anticipate that this study will be useful for future studies that examine the spread of infections from wild animals to humans in general and those studying ZIKV in primates in particular. Podcast: A podcast concerning this article is available.Connor R. BuechlerAdam L. BaileyAndrea M. WeilerGabrielle L. BarryMeghan E. BreitbachLaurel M. StewartAnna J. JasinskaNelson B. FreimerCristian ApetreiJane E. Phillips-ConroyClifford J. JollyJeffrey RogersThomas C. FriedrichDavid H. O’ConnorAmerican Society for Microbiologyarticlefalse-positive reactionsFlavivirussensitivity and specificityseroepidemiologic studiesZika virusZika virus infectionMicrobiologyQR1-502ENmSphere, Vol 2, Iss 2 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	false-positive reactions Flavivirus sensitivity and specificity seroepidemiologic studies Zika virus Zika virus infection Microbiology QR1-502
spellingShingle	false-positive reactions Flavivirus sensitivity and specificity seroepidemiologic studies Zika virus Zika virus infection Microbiology QR1-502 Connor R. Buechler Adam L. Bailey Andrea M. Weiler Gabrielle L. Barry Meghan E. Breitbach Laurel M. Stewart Anna J. Jasinska Nelson B. Freimer Cristian Apetrei Jane E. Phillips-Conroy Clifford J. Jolly Jeffrey Rogers Thomas C. Friedrich David H. O’Connor Seroprevalence of Zika Virus in Wild African Green Monkeys and Baboons
description	ABSTRACT Zika virus (ZIKV) has recently spread through the Americas and has been associated with a range of health effects, including birth defects in children born to women infected during pregnancy. Although the natural reservoir of ZIKV remains poorly defined, the virus was first identified in a captive “sentinel” macaque monkey in Africa in 1947. However, the virus has not been reported in humans or nonhuman primates (NHPs) in Africa outside Gabon in over a decade. Here, we examine ZIKV infection in 239 wild baboons and African green monkeys from South Africa, the Gambia, Tanzania, and Zambia using combinations of unbiased deep sequencing, quantitative reverse transcription-PCR (qRT-PCR), and an antibody capture assay that we optimized using serum collected from captive macaque monkeys exposed to ZIKV, dengue virus, and yellow fever virus. While we did not find evidence of active ZIKV infection in wild NHPs in Africa, we found variable ZIKV seropositivity of up to 16% in some of the NHP populations sampled. We anticipate that these results and the methodology described within will help in continued efforts to determine the prevalence, natural reservoir, and transmission dynamics of ZIKV in Africa and elsewhere. IMPORTANCE Zika virus (ZIKV) is a mosquito-borne virus originally discovered in a captive monkey living in the Zika Forest of Uganda, Africa, in 1947. Recently, an outbreak in South America has shown that ZIKV infection can cause myriad health effects, including birth defects in the children of women infected during pregnancy. Here, we sought to investigate ZIKV infection in wild African primates to better understand its emergence and spread, looking for evidence of active or prior infection. Our results suggest that up to 16% of some populations of nonhuman primate were, at some point, exposed to ZIKV. We anticipate that this study will be useful for future studies that examine the spread of infections from wild animals to humans in general and those studying ZIKV in primates in particular. Podcast: A podcast concerning this article is available.
format	article
author	Connor R. Buechler Adam L. Bailey Andrea M. Weiler Gabrielle L. Barry Meghan E. Breitbach Laurel M. Stewart Anna J. Jasinska Nelson B. Freimer Cristian Apetrei Jane E. Phillips-Conroy Clifford J. Jolly Jeffrey Rogers Thomas C. Friedrich David H. O’Connor
author_facet	Connor R. Buechler Adam L. Bailey Andrea M. Weiler Gabrielle L. Barry Meghan E. Breitbach Laurel M. Stewart Anna J. Jasinska Nelson B. Freimer Cristian Apetrei Jane E. Phillips-Conroy Clifford J. Jolly Jeffrey Rogers Thomas C. Friedrich David H. O’Connor
author_sort	Connor R. Buechler
title	Seroprevalence of Zika Virus in Wild African Green Monkeys and Baboons
title_short	Seroprevalence of Zika Virus in Wild African Green Monkeys and Baboons
title_full	Seroprevalence of Zika Virus in Wild African Green Monkeys and Baboons
title_fullStr	Seroprevalence of Zika Virus in Wild African Green Monkeys and Baboons
title_full_unstemmed	Seroprevalence of Zika Virus in Wild African Green Monkeys and Baboons
title_sort	seroprevalence of zika virus in wild african green monkeys and baboons
publisher	American Society for Microbiology
publishDate	2017
url	https://doaj.org/article/e68291cb6bea452e96771d33b7c28b5c
work_keys_str_mv	AT connorrbuechler seroprevalenceofzikavirusinwildafricangreenmonkeysandbaboons AT adamlbailey seroprevalenceofzikavirusinwildafricangreenmonkeysandbaboons AT andreamweiler seroprevalenceofzikavirusinwildafricangreenmonkeysandbaboons AT gabriellelbarry seroprevalenceofzikavirusinwildafricangreenmonkeysandbaboons AT meghanebreitbach seroprevalenceofzikavirusinwildafricangreenmonkeysandbaboons AT laurelmstewart seroprevalenceofzikavirusinwildafricangreenmonkeysandbaboons AT annajjasinska seroprevalenceofzikavirusinwildafricangreenmonkeysandbaboons AT nelsonbfreimer seroprevalenceofzikavirusinwildafricangreenmonkeysandbaboons AT cristianapetrei seroprevalenceofzikavirusinwildafricangreenmonkeysandbaboons AT janeephillipsconroy seroprevalenceofzikavirusinwildafricangreenmonkeysandbaboons AT cliffordjjolly seroprevalenceofzikavirusinwildafricangreenmonkeysandbaboons AT jeffreyrogers seroprevalenceofzikavirusinwildafricangreenmonkeysandbaboons AT thomascfriedrich seroprevalenceofzikavirusinwildafricangreenmonkeysandbaboons AT davidhoconnor seroprevalenceofzikavirusinwildafricangreenmonkeysandbaboons
_version_	1718428163751542784

Seroprevalence of Zika Virus in Wild African Green Monkeys and Baboons

Ejemplares similares