Viral paratransgenesis in the malaria vector Anopheles gambiae.

Paratransgenesis, the genetic manipulation of insect symbiotic microorganisms, is being considered as a potential method to control vector-borne diseases such as malaria. The feasibility of paratransgenic malaria control has been hampered by the lack of candidate symbiotic microorganisms for the maj...

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Autores principales:	Xiaoxia Ren, Egbert Hoiczyk, Jason L Rasgon
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/e687ea1a686545849f74aec407a8bdc3
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spelling	oai:doaj.org-article:e687ea1a686545849f74aec407a8bdc32021-11-25T05:47:31ZViral paratransgenesis in the malaria vector Anopheles gambiae.1553-73661553-737410.1371/journal.ppat.1000135https://doaj.org/article/e687ea1a686545849f74aec407a8bdc32008-08-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18725926/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Paratransgenesis, the genetic manipulation of insect symbiotic microorganisms, is being considered as a potential method to control vector-borne diseases such as malaria. The feasibility of paratransgenic malaria control has been hampered by the lack of candidate symbiotic microorganisms for the major vector Anopheles gambiae. In other systems, densonucleosis viruses (DNVs) are attractive agents for viral paratransgenesis because they infect important vector insects, can be genetically manipulated and are transmitted to subsequent generations. However, An. gambiae has been shown to be refractory to DNV dissemination. We discovered, cloned and characterized the first known DNV (AgDNV) capable of infection and dissemination in An. gambiae. We developed a flexible AgDNV-based expression vector to express any gene of interest in An. gambiae using a two-plasmid helper-transducer system. To demonstrate proof-of-concept of the viral paratransgenesis strategy, we used this system to transduce expression of an exogenous gene (enhanced green fluorescent protein; EGFP) in An. gambiae mosquitoes. Wild-type and EGFP-transducing AgDNV virions were highly infectious to An. gambiae larvae, disseminated to and expressed EGFP in epidemiologically relevant adult tissues such as midgut, fat body and ovaries and were transmitted to subsequent mosquito generations. These proof-of-principle data suggest that AgDNV could be used as part of a paratransgenic malaria control strategy by transduction of anti-Plasmodium peptides or insect-specific toxins in Anopheles mosquitoes. AgDNV will also be extremely valuable as an effective and easy-to-use laboratory tool for transient gene expression or RNAi in An. gambiae.Xiaoxia RenEgbert HoiczykJason L RasgonPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 4, Iss 8, p e1000135 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Xiaoxia Ren Egbert Hoiczyk Jason L Rasgon Viral paratransgenesis in the malaria vector Anopheles gambiae.
description	Paratransgenesis, the genetic manipulation of insect symbiotic microorganisms, is being considered as a potential method to control vector-borne diseases such as malaria. The feasibility of paratransgenic malaria control has been hampered by the lack of candidate symbiotic microorganisms for the major vector Anopheles gambiae. In other systems, densonucleosis viruses (DNVs) are attractive agents for viral paratransgenesis because they infect important vector insects, can be genetically manipulated and are transmitted to subsequent generations. However, An. gambiae has been shown to be refractory to DNV dissemination. We discovered, cloned and characterized the first known DNV (AgDNV) capable of infection and dissemination in An. gambiae. We developed a flexible AgDNV-based expression vector to express any gene of interest in An. gambiae using a two-plasmid helper-transducer system. To demonstrate proof-of-concept of the viral paratransgenesis strategy, we used this system to transduce expression of an exogenous gene (enhanced green fluorescent protein; EGFP) in An. gambiae mosquitoes. Wild-type and EGFP-transducing AgDNV virions were highly infectious to An. gambiae larvae, disseminated to and expressed EGFP in epidemiologically relevant adult tissues such as midgut, fat body and ovaries and were transmitted to subsequent mosquito generations. These proof-of-principle data suggest that AgDNV could be used as part of a paratransgenic malaria control strategy by transduction of anti-Plasmodium peptides or insect-specific toxins in Anopheles mosquitoes. AgDNV will also be extremely valuable as an effective and easy-to-use laboratory tool for transient gene expression or RNAi in An. gambiae.
format	article
author	Xiaoxia Ren Egbert Hoiczyk Jason L Rasgon
author_facet	Xiaoxia Ren Egbert Hoiczyk Jason L Rasgon
author_sort	Xiaoxia Ren
title	Viral paratransgenesis in the malaria vector Anopheles gambiae.
title_short	Viral paratransgenesis in the malaria vector Anopheles gambiae.
title_full	Viral paratransgenesis in the malaria vector Anopheles gambiae.
title_fullStr	Viral paratransgenesis in the malaria vector Anopheles gambiae.
title_full_unstemmed	Viral paratransgenesis in the malaria vector Anopheles gambiae.
title_sort	viral paratransgenesis in the malaria vector anopheles gambiae.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/e687ea1a686545849f74aec407a8bdc3
work_keys_str_mv	AT xiaoxiaren viralparatransgenesisinthemalariavectoranophelesgambiae AT egberthoiczyk viralparatransgenesisinthemalariavectoranophelesgambiae AT jasonlrasgon viralparatransgenesisinthemalariavectoranophelesgambiae
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Viral paratransgenesis in the malaria vector Anopheles gambiae.

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