Growth and fosE gene expression in inulin-containing medium of two strains of Lactobacillus casei originated from the human intestinal tract

Abstract. Authors. 2019. Growth and fosE gene expression in inulin-containing medium of two strains of Lactobacillus casei originated from the human intestinal tract. Biodiversitas 20: 2758-2763. Inulin is a fructooligosaccharide consisting of a fructose monomer with ?- (1,2) glycosidic linkage. The...

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Autores principales:	AMIK CHOIRUL AFIDAH, TUTIK DWI WAHYUNINGSIH, Widodo Widodo
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	β-fructoside; β-glicoside; fose; inulin Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/e689bcf382c9440e9fbedec3b3f3a3eb
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spelling	oai:doaj.org-article:e689bcf382c9440e9fbedec3b3f3a3eb2021-11-21T21:54:25ZGrowth and fosE gene expression in inulin-containing medium of two strains of Lactobacillus casei originated from the human intestinal tract1412-033X2085-472210.13057/biodiv/d200942https://doaj.org/article/e689bcf382c9440e9fbedec3b3f3a3eb2019-08-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4197https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Authors. 2019. Growth and fosE gene expression in inulin-containing medium of two strains of Lactobacillus casei originated from the human intestinal tract. Biodiversitas 20: 2758-2763. Inulin is a fructooligosaccharide consisting of a fructose monomer with ?- (1,2) glycosidic linkage. The human colon cannot degrade inulin because it lacks the enzyme that degrades ?- (1,2) glycosidic linkage. Lactobacillus casei strain AP is able to grow in inulin, L. casei strain AF grows gradually. The ability to grow in inulin is proposed by the fosE gene, which hydrolyzes ?- (1,2) glycosidic linkage. The present study aimed to detect and measure fosE gene expression in inulin. Extracellular and intracellular inulin and fructose concentrations were calculated using high-performance liquid chromatography. The reduction of inulin concentration in L. casei strain AP was higher than that in L. casei strain AF. Extracellular fructose concentration was lower in L. casei strain AP because fructose from inulin degradation was transported into the cell. No fructose was detected inside Lactobacillus cells. The fosE gene was detected in L. casei strains AP and AF with a length of 229 bp. fosE gene expression was up-regulated 5.9-fold in L. casei strain AP and 1.7-fold in L. casei strain AF and was three times higher in L. casei strain AP than that in L. casei strain AF, suggesting that the former metabolizes inulin better than the latter.AMIK CHOIRUL AFIDAHTUTIK DWI WAHYUNINGSIHWidodo WidodoMBI & UNS Soloarticleβ-fructoside; β-glicoside; fose; inulinBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 9 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	β-fructoside; β-glicoside; fose; inulin Biology (General) QH301-705.5
spellingShingle	β-fructoside; β-glicoside; fose; inulin Biology (General) QH301-705.5 AMIK CHOIRUL AFIDAH TUTIK DWI WAHYUNINGSIH Widodo Widodo Growth and fosE gene expression in inulin-containing medium of two strains of Lactobacillus casei originated from the human intestinal tract
description	Abstract. Authors. 2019. Growth and fosE gene expression in inulin-containing medium of two strains of Lactobacillus casei originated from the human intestinal tract. Biodiversitas 20: 2758-2763. Inulin is a fructooligosaccharide consisting of a fructose monomer with ?- (1,2) glycosidic linkage. The human colon cannot degrade inulin because it lacks the enzyme that degrades ?- (1,2) glycosidic linkage. Lactobacillus casei strain AP is able to grow in inulin, L. casei strain AF grows gradually. The ability to grow in inulin is proposed by the fosE gene, which hydrolyzes ?- (1,2) glycosidic linkage. The present study aimed to detect and measure fosE gene expression in inulin. Extracellular and intracellular inulin and fructose concentrations were calculated using high-performance liquid chromatography. The reduction of inulin concentration in L. casei strain AP was higher than that in L. casei strain AF. Extracellular fructose concentration was lower in L. casei strain AP because fructose from inulin degradation was transported into the cell. No fructose was detected inside Lactobacillus cells. The fosE gene was detected in L. casei strains AP and AF with a length of 229 bp. fosE gene expression was up-regulated 5.9-fold in L. casei strain AP and 1.7-fold in L. casei strain AF and was three times higher in L. casei strain AP than that in L. casei strain AF, suggesting that the former metabolizes inulin better than the latter.
format	article
author	AMIK CHOIRUL AFIDAH TUTIK DWI WAHYUNINGSIH Widodo Widodo
author_facet	AMIK CHOIRUL AFIDAH TUTIK DWI WAHYUNINGSIH Widodo Widodo
author_sort	AMIK CHOIRUL AFIDAH
title	Growth and fosE gene expression in inulin-containing medium of two strains of Lactobacillus casei originated from the human intestinal tract
title_short	Growth and fosE gene expression in inulin-containing medium of two strains of Lactobacillus casei originated from the human intestinal tract
title_full	Growth and fosE gene expression in inulin-containing medium of two strains of Lactobacillus casei originated from the human intestinal tract
title_fullStr	Growth and fosE gene expression in inulin-containing medium of two strains of Lactobacillus casei originated from the human intestinal tract
title_full_unstemmed	Growth and fosE gene expression in inulin-containing medium of two strains of Lactobacillus casei originated from the human intestinal tract
title_sort	growth and fose gene expression in inulin-containing medium of two strains of lactobacillus casei originated from the human intestinal tract
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/e689bcf382c9440e9fbedec3b3f3a3eb
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Growth and fosE gene expression in inulin-containing medium of two strains of Lactobacillus casei originated from the human intestinal tract

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