High-Intensity Interval Training Reversed High-Fat Diet–Induced M1-Macrophage Polarization in Rat Adipose Tissue via Inhibition of NOTCH Signaling

High-Intensity Interval Training Reversed High-Fat Diet–Induced M1-Macrophage Polarization in Rat Adipose Tissue via Inhibition of NOTCH Signaling

Mehrnoosh Shanaki, 1 Maryam Khosravi, 2 Arezoo Khoshdooni-Farahani, 3 Alireza Dadashi, 4 Mohammad Foad Heydari, 5 Maryam Delfan, 6 Hanieh Jafary, 3 Sattar Gorgani-Firuzjaee 5 1Department of Medical Laboratory Sciences, School of Allied Health Medicine, Shahid Beheshti University of Medical Sciences...

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Autores principales: Shanaki M, Khosravi M, Khoshdooni-Farahani A, Dadashi A, Heydari MF, Delfan M, Jafary H, Gorgani-Firuzjaee S
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2020
Materias:
obesity
diabetes
macrophage polarization
high intensity interval training
continuous endurance training
Pathology
RB1-214
Therapeutics. Pharmacology
RM1-950
Acceso en línea:https://doaj.org/article/e68eab9b12684341b3a3449812f2f5c3
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spelling oai:doaj.org-article:e68eab9b12684341b3a3449812f2f5c32021-12-02T09:42:52ZHigh-Intensity Interval Training Reversed High-Fat Diet–Induced M1-Macrophage Polarization in Rat Adipose Tissue via Inhibition of NOTCH Signaling1178-7031https://doaj.org/article/e68eab9b12684341b3a3449812f2f5c32020-03-01T00:00:00Zhttps://www.dovepress.com/high-intensity-interval-training-reversed-high-fat-diet-induced-m1-mac-peer-reviewed-article-JIRhttps://doaj.org/toc/1178-7031Mehrnoosh Shanaki, 1 Maryam Khosravi, 2 Arezoo Khoshdooni-Farahani, 3 Alireza Dadashi, 4 Mohammad Foad Heydari, 5 Maryam Delfan, 6 Hanieh Jafary, 3 Sattar Gorgani-Firuzjaee 5 1Department of Medical Laboratory Sciences, School of Allied Health Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 2Department of Immunology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; 3Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran; 4Department of Infectious Disease, School of Medicine, AJA University of Medical Sciences, Tehran, Iran; 5Department of Medical Laboratory Sciences, School of Allied Health Medicine, AJA University of Medical Sciences, Tehran, Iran; 6Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Alzahra University, Tehran, IranCorrespondence: Sattar Gorgani-Firuzjaee Email s-gorganif@alumnus.tums.ac.irIntroduction: There is accumulating evidence on the beneficial effect of exercise intervention in the management of metabolic disorders; however, the molecular mechanism is still unclear. Here, the current study aimed to compare the effect of high-intensity interval training (HIIT) and continuous endurance training (CET) on serum and adipose-tissue markers of M1/M2 macrophage polarization.Methods: A total of  45 healthy male Wistar rats were divided into groups of normal chow (n=10) and high-fat diet (HFD) (n=35). Then, rats receiving the HFD were randomly divided into four groups. Training programs were performed for 5 days/week over 10 weeks. The CET protocol included 30 minutes running at 50%– 60% of VO 2max. The HIIT protocol consisted of five repeated intervals of 2-minute sprints on the treadmill at 80%– 90% VO 2max workload with 1 minute’s 30%– 35% VO 2max interval for each rat. Then, biochemical parameters were assessed. Macrophage-polarization markers were assessed at mRNA and protein levels by real-time PCR and Western blotting, respectively.Results: Both exercise-training programs, especially HIIT, reversed increased serum biochemical parameters (glucose, triglycerides, cholesterol, Homeostatic Model Assessment of Insulin Resistance, and hsCRP), M1-polarization markers (circulating IL6, TNFα, and adipose-tissue mRNA expression of IL6, TNFα and iNOS), M2 markers (CD206, CD163, and IL10 expression), as well as pIκKB, pNFκB, and NICD expression in HFD-induced diabetes.Conclusion: Our findings suggest that despite devoting less time, the HIIT workout is a more effective intervention for diabetes management. Moreover, HIIT reverses HFD-induced macrophage polarization by targeting the NFκB and NOTCH signaling pathways.Keywords: obesity, diabetes, macrophage polarization, high-intensity interval training, continuous endurance trainingShanaki MKhosravi MKhoshdooni-Farahani ADadashi AHeydari MFDelfan MJafary HGorgani-Firuzjaee SDove Medical Pressarticleobesitydiabetesmacrophage polarizationhigh intensity interval trainingcontinuous endurance trainingPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 13, Pp 165-174 (2020)
institution DOAJ
collection DOAJ
language EN
topic obesity
diabetes
macrophage polarization
high intensity interval training
continuous endurance training
Pathology
RB1-214
Therapeutics. Pharmacology
RM1-950
spellingShingle obesity
diabetes
macrophage polarization
high intensity interval training
continuous endurance training
Pathology
RB1-214
Therapeutics. Pharmacology
RM1-950
Shanaki M
Khosravi M
Khoshdooni-Farahani A
Dadashi A
Heydari MF
Delfan M
Jafary H
Gorgani-Firuzjaee S
High-Intensity Interval Training Reversed High-Fat Diet–Induced M1-Macrophage Polarization in Rat Adipose Tissue via Inhibition of NOTCH Signaling
description Mehrnoosh Shanaki, 1 Maryam Khosravi, 2 Arezoo Khoshdooni-Farahani, 3 Alireza Dadashi, 4 Mohammad Foad Heydari, 5 Maryam Delfan, 6 Hanieh Jafary, 3 Sattar Gorgani-Firuzjaee 5 1Department of Medical Laboratory Sciences, School of Allied Health Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 2Department of Immunology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; 3Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran; 4Department of Infectious Disease, School of Medicine, AJA University of Medical Sciences, Tehran, Iran; 5Department of Medical Laboratory Sciences, School of Allied Health Medicine, AJA University of Medical Sciences, Tehran, Iran; 6Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Alzahra University, Tehran, IranCorrespondence: Sattar Gorgani-Firuzjaee Email s-gorganif@alumnus.tums.ac.irIntroduction: There is accumulating evidence on the beneficial effect of exercise intervention in the management of metabolic disorders; however, the molecular mechanism is still unclear. Here, the current study aimed to compare the effect of high-intensity interval training (HIIT) and continuous endurance training (CET) on serum and adipose-tissue markers of M1/M2 macrophage polarization.Methods: A total of  45 healthy male Wistar rats were divided into groups of normal chow (n=10) and high-fat diet (HFD) (n=35). Then, rats receiving the HFD were randomly divided into four groups. Training programs were performed for 5 days/week over 10 weeks. The CET protocol included 30 minutes running at 50%– 60% of VO 2max. The HIIT protocol consisted of five repeated intervals of 2-minute sprints on the treadmill at 80%– 90% VO 2max workload with 1 minute’s 30%– 35% VO 2max interval for each rat. Then, biochemical parameters were assessed. Macrophage-polarization markers were assessed at mRNA and protein levels by real-time PCR and Western blotting, respectively.Results: Both exercise-training programs, especially HIIT, reversed increased serum biochemical parameters (glucose, triglycerides, cholesterol, Homeostatic Model Assessment of Insulin Resistance, and hsCRP), M1-polarization markers (circulating IL6, TNFα, and adipose-tissue mRNA expression of IL6, TNFα and iNOS), M2 markers (CD206, CD163, and IL10 expression), as well as pIκKB, pNFκB, and NICD expression in HFD-induced diabetes.Conclusion: Our findings suggest that despite devoting less time, the HIIT workout is a more effective intervention for diabetes management. Moreover, HIIT reverses HFD-induced macrophage polarization by targeting the NFκB and NOTCH signaling pathways.Keywords: obesity, diabetes, macrophage polarization, high-intensity interval training, continuous endurance training
format article
author Shanaki M
Khosravi M
Khoshdooni-Farahani A
Dadashi A
Heydari MF
Delfan M
Jafary H
Gorgani-Firuzjaee S
author_facet Shanaki M
Khosravi M
Khoshdooni-Farahani A
Dadashi A
Heydari MF
Delfan M
Jafary H
Gorgani-Firuzjaee S
author_sort Shanaki M
title High-Intensity Interval Training Reversed High-Fat Diet–Induced M1-Macrophage Polarization in Rat Adipose Tissue via Inhibition of NOTCH Signaling
title_short High-Intensity Interval Training Reversed High-Fat Diet–Induced M1-Macrophage Polarization in Rat Adipose Tissue via Inhibition of NOTCH Signaling
title_full High-Intensity Interval Training Reversed High-Fat Diet–Induced M1-Macrophage Polarization in Rat Adipose Tissue via Inhibition of NOTCH Signaling
title_fullStr High-Intensity Interval Training Reversed High-Fat Diet–Induced M1-Macrophage Polarization in Rat Adipose Tissue via Inhibition of NOTCH Signaling
title_full_unstemmed High-Intensity Interval Training Reversed High-Fat Diet–Induced M1-Macrophage Polarization in Rat Adipose Tissue via Inhibition of NOTCH Signaling
title_sort high-intensity interval training reversed high-fat diet–induced m1-macrophage polarization in rat adipose tissue via inhibition of notch signaling
publisher Dove Medical Press
publishDate 2020
url https://doaj.org/article/e68eab9b12684341b3a3449812f2f5c3
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