Adhesion proteins--an impact on skeletal myoblast differentiation.

Formation of mammalian skeletal muscle myofibers, that takes place during embryogenesis, muscle growth or regeneration, requires precise regulation of myoblast adhesion and fusion. There are few evidences showing that adhesion proteins play important role in both processes. To follow the function of...

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Autores principales:	Marta Przewoźniak, Iwona Czaplicka, Areta M Czerwińska, Agnieszka Markowska-Zagrajek, Jerzy Moraczewski, Władysława Stremińska, Katarzyna Jańczyk-Ilach, Maria A Ciemerych, Edyta Brzoska
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e68f0ccaa7fc4344b724f36bf72119da
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spelling	oai:doaj.org-article:e68f0ccaa7fc4344b724f36bf72119da2021-11-18T07:46:50ZAdhesion proteins--an impact on skeletal myoblast differentiation.1932-620310.1371/journal.pone.0061760https://doaj.org/article/e68f0ccaa7fc4344b724f36bf72119da2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23671573/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Formation of mammalian skeletal muscle myofibers, that takes place during embryogenesis, muscle growth or regeneration, requires precise regulation of myoblast adhesion and fusion. There are few evidences showing that adhesion proteins play important role in both processes. To follow the function of these molecules in myoblast differentiation we analysed integrin alpha3, integrin beta1, ADAM12, CD9, CD81, M-cadherin, and VCAM-1 during muscle regeneration. We showed that increase in the expression of these proteins accompanies myoblast fusion and myotube formation in vivo. We also showed that during myoblast fusion in vitro integrin alpha3 associates with integrin beta1 and ADAM12, and also CD9 and CD81, but not with M-cadherin or VCAM-1. Moreover, we documented that experimental modification in the expression of integrin alpha3 lead to the modification of myoblast fusion in vitro. Underexpression of integrin alpha3 decreased myoblasts' ability to fuse. This phenomenon was not related to the modifications in the expression of other adhesion proteins, i.e. integrin beta1, CD9, CD81, ADAM12, M-cadherin, or VCAM-1. Apparently, aberrant expression only of one partner of multiprotein adhesion complexes necessary for myoblast fusion, in this case integrin alpha3, prevents its proper function. Summarizing, we demonstrated the importance of analysed adhesion proteins in myoblast fusion both in vivo and in vitro.Marta PrzewoźniakIwona CzaplickaAreta M CzerwińskaAgnieszka Markowska-ZagrajekJerzy MoraczewskiWładysława StremińskaKatarzyna Jańczyk-IlachMaria A CiemerychMaria A CiemerychEdyta BrzoskaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e61760 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marta Przewoźniak Iwona Czaplicka Areta M Czerwińska Agnieszka Markowska-Zagrajek Jerzy Moraczewski Władysława Stremińska Katarzyna Jańczyk-Ilach Maria A Ciemerych Maria A Ciemerych Edyta Brzoska Adhesion proteins--an impact on skeletal myoblast differentiation.
description	Formation of mammalian skeletal muscle myofibers, that takes place during embryogenesis, muscle growth or regeneration, requires precise regulation of myoblast adhesion and fusion. There are few evidences showing that adhesion proteins play important role in both processes. To follow the function of these molecules in myoblast differentiation we analysed integrin alpha3, integrin beta1, ADAM12, CD9, CD81, M-cadherin, and VCAM-1 during muscle regeneration. We showed that increase in the expression of these proteins accompanies myoblast fusion and myotube formation in vivo. We also showed that during myoblast fusion in vitro integrin alpha3 associates with integrin beta1 and ADAM12, and also CD9 and CD81, but not with M-cadherin or VCAM-1. Moreover, we documented that experimental modification in the expression of integrin alpha3 lead to the modification of myoblast fusion in vitro. Underexpression of integrin alpha3 decreased myoblasts' ability to fuse. This phenomenon was not related to the modifications in the expression of other adhesion proteins, i.e. integrin beta1, CD9, CD81, ADAM12, M-cadherin, or VCAM-1. Apparently, aberrant expression only of one partner of multiprotein adhesion complexes necessary for myoblast fusion, in this case integrin alpha3, prevents its proper function. Summarizing, we demonstrated the importance of analysed adhesion proteins in myoblast fusion both in vivo and in vitro.
format	article
author	Marta Przewoźniak Iwona Czaplicka Areta M Czerwińska Agnieszka Markowska-Zagrajek Jerzy Moraczewski Władysława Stremińska Katarzyna Jańczyk-Ilach Maria A Ciemerych Maria A Ciemerych Edyta Brzoska
author_facet	Marta Przewoźniak Iwona Czaplicka Areta M Czerwińska Agnieszka Markowska-Zagrajek Jerzy Moraczewski Władysława Stremińska Katarzyna Jańczyk-Ilach Maria A Ciemerych Maria A Ciemerych Edyta Brzoska
author_sort	Marta Przewoźniak
title	Adhesion proteins--an impact on skeletal myoblast differentiation.
title_short	Adhesion proteins--an impact on skeletal myoblast differentiation.
title_full	Adhesion proteins--an impact on skeletal myoblast differentiation.
title_fullStr	Adhesion proteins--an impact on skeletal myoblast differentiation.
title_full_unstemmed	Adhesion proteins--an impact on skeletal myoblast differentiation.
title_sort	adhesion proteins--an impact on skeletal myoblast differentiation.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/e68f0ccaa7fc4344b724f36bf72119da
work_keys_str_mv	AT martaprzewozniak adhesionproteinsanimpactonskeletalmyoblastdifferentiation AT iwonaczaplicka adhesionproteinsanimpactonskeletalmyoblastdifferentiation AT aretamczerwinska adhesionproteinsanimpactonskeletalmyoblastdifferentiation AT agnieszkamarkowskazagrajek adhesionproteinsanimpactonskeletalmyoblastdifferentiation AT jerzymoraczewski adhesionproteinsanimpactonskeletalmyoblastdifferentiation AT władysławastreminska adhesionproteinsanimpactonskeletalmyoblastdifferentiation AT katarzynajanczykilach adhesionproteinsanimpactonskeletalmyoblastdifferentiation AT mariaaciemerych adhesionproteinsanimpactonskeletalmyoblastdifferentiation AT mariaaciemerych adhesionproteinsanimpactonskeletalmyoblastdifferentiation AT edytabrzoska adhesionproteinsanimpactonskeletalmyoblastdifferentiation
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Adhesion proteins--an impact on skeletal myoblast differentiation.

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