The use of multiplex-PCR method in identification of Candida species from vaginal candidiasis patients

Abstract. Susilawati, Rahadiyanto KY, Ramdja, Theodorus, Hermansyah. 2019. The use of multiplex-PCR method in identification of Candida species from vaginal candidiasis patients. Biodiversitas 20: 3063-3069. Vaginal Candidiasis is an infection caused by a yeast called Candida which may be resulted i...

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Autores principales:	SUSILAWATI, KEMAS YA’KUB RAHADIYANTO, MUHAIMIN RAMDJA, THEODORUS, Hermansyah Hermansyah
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	candida, candidiasis, multiplex-pcr, fermentation Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/e690444d279841d9ac7427de7ff3280a
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spelling	oai:doaj.org-article:e690444d279841d9ac7427de7ff3280a2021-11-21T21:54:57ZThe use of multiplex-PCR method in identification of Candida species from vaginal candidiasis patients1412-033X2085-472210.13057/biodiv/d201040https://doaj.org/article/e690444d279841d9ac7427de7ff3280a2019-09-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4091https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Susilawati, Rahadiyanto KY, Ramdja, Theodorus, Hermansyah. 2019. The use of multiplex-PCR method in identification of Candida species from vaginal candidiasis patients. Biodiversitas 20: 3063-3069. Vaginal Candidiasis is an infection caused by a yeast called Candida which may be resulted in different virulences, thus leading to a varied drug of choice for therapy. The objective of this study was to determine the sensitivity and specificity of multiplex-PCR test in detecting Candida species in women with clinical diagnosis of vaginal candidiasis. There were 79 vaginal swab samples which fulfill inclusion criteria. Samples were cultured at ASD media for isolation of Candida spp. Identification of Candida spp. was conducted using both biochemical fermentation and multiplex-PCR methods. Primers pairs used in multiplex-PCR were universal primer ITS1 and ITS2, and specific primer CA3 and CA4. The identification test of multiplex-PCR resulted in Candida krusei: sensitivity: 100%, specificity:61,1%, Positive Prediction Value (PPV):63.2%, Negative Prediction Value (NPV): 100%; C. glabrata: sensitivity:0%, specificity: 100% PPV: 0% NPV: 76.7%; C. tropicalis: sensitivity:100%, spesificity:100%, PPV: 100% NPV: 100%; C. albicans: sensitivity: 33.3%, specificity: 100%, PPV: 100%, NPV: 93.1% and C. stellatoidea: sensitivity:: 100%, specificity:92.6%, PPV: 60%, NPV: 100%. These results suggested that multiplex-PCR method becomes a rapid alternative in identifying species of Candida due to its high sensitivity and high specificity.SUSILAWATIKEMAS YA’KUB RAHADIYANTOMUHAIMIN RAMDJATHEODORUSHermansyah HermansyahMBI & UNS Soloarticlecandida, candidiasis, multiplex-pcr, fermentationBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 10 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	candida, candidiasis, multiplex-pcr, fermentation Biology (General) QH301-705.5
spellingShingle	candida, candidiasis, multiplex-pcr, fermentation Biology (General) QH301-705.5 SUSILAWATI KEMAS YA’KUB RAHADIYANTO MUHAIMIN RAMDJA THEODORUS Hermansyah Hermansyah The use of multiplex-PCR method in identification of Candida species from vaginal candidiasis patients
description	Abstract. Susilawati, Rahadiyanto KY, Ramdja, Theodorus, Hermansyah. 2019. The use of multiplex-PCR method in identification of Candida species from vaginal candidiasis patients. Biodiversitas 20: 3063-3069. Vaginal Candidiasis is an infection caused by a yeast called Candida which may be resulted in different virulences, thus leading to a varied drug of choice for therapy. The objective of this study was to determine the sensitivity and specificity of multiplex-PCR test in detecting Candida species in women with clinical diagnosis of vaginal candidiasis. There were 79 vaginal swab samples which fulfill inclusion criteria. Samples were cultured at ASD media for isolation of Candida spp. Identification of Candida spp. was conducted using both biochemical fermentation and multiplex-PCR methods. Primers pairs used in multiplex-PCR were universal primer ITS1 and ITS2, and specific primer CA3 and CA4. The identification test of multiplex-PCR resulted in Candida krusei: sensitivity: 100%, specificity:61,1%, Positive Prediction Value (PPV):63.2%, Negative Prediction Value (NPV): 100%; C. glabrata: sensitivity:0%, specificity: 100% PPV: 0% NPV: 76.7%; C. tropicalis: sensitivity:100%, spesificity:100%, PPV: 100% NPV: 100%; C. albicans: sensitivity: 33.3%, specificity: 100%, PPV: 100%, NPV: 93.1% and C. stellatoidea: sensitivity:: 100%, specificity:92.6%, PPV: 60%, NPV: 100%. These results suggested that multiplex-PCR method becomes a rapid alternative in identifying species of Candida due to its high sensitivity and high specificity.
format	article
author	SUSILAWATI KEMAS YA’KUB RAHADIYANTO MUHAIMIN RAMDJA THEODORUS Hermansyah Hermansyah
author_facet	SUSILAWATI KEMAS YA’KUB RAHADIYANTO MUHAIMIN RAMDJA THEODORUS Hermansyah Hermansyah
author_sort	SUSILAWATI
title	The use of multiplex-PCR method in identification of Candida species from vaginal candidiasis patients
title_short	The use of multiplex-PCR method in identification of Candida species from vaginal candidiasis patients
title_full	The use of multiplex-PCR method in identification of Candida species from vaginal candidiasis patients
title_fullStr	The use of multiplex-PCR method in identification of Candida species from vaginal candidiasis patients
title_full_unstemmed	The use of multiplex-PCR method in identification of Candida species from vaginal candidiasis patients
title_sort	use of multiplex-pcr method in identification of candida species from vaginal candidiasis patients
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/e690444d279841d9ac7427de7ff3280a
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The use of multiplex-PCR method in identification of Candida species from vaginal candidiasis patients

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