Sets of covariant residues modulate the activity and thermal stability of GH1 β-glucosidases.
The statistical coupling analysis of 768 β-glucosidases from the GH1 family revealed 23 positions in which the amino acid frequencies are coupled. The roles of these covariant positions in terms of the properties of β-glucosidases were investigated by alanine-screening mutagenesis using the fall arm...
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| Auteurs principaux: | , , , |
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| Format: | article |
| Langue: | EN |
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Public Library of Science (PLoS)
2014
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| Accès en ligne: | https://doaj.org/article/e6912e8aa6554dbaaa5cc7c75985a4b2 |
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