Circular RNA circ_0000517 Facilitates The Growth and Metastasis of Non-Small Cell Lung Cancer by Sponging miR-326/miR-330-5p

Objective: There is growing evidence showing that circular RNAs (circRNAs) are crucial regulators in modulating the biological behavior of tumors. This work is aimed to probe the role of circ_0000517 in non-small cell lung cancer (NSCLC) and to elucidate its mechanism of action. Materials and Metho...

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Autores principales:	Qiyan Tan, Changyu Liu, Ying Shen, Tao Huang
Formato:	article
Lenguaje:	EN
Publicado:	Royan Institute (ACECR), Tehran 2021
Materias:	mir-326 mir-330-5p mmp2 non-small cell lung cancer Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e6965916b8224b0991c682bb7bbd8be9
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spelling	oai:doaj.org-article:e6965916b8224b0991c682bb7bbd8be92021-11-07T07:33:14ZCircular RNA circ_0000517 Facilitates The Growth and Metastasis of Non-Small Cell Lung Cancer by Sponging miR-326/miR-330-5p10.22074/cellj.2021.79132228-58062228-5814https://doaj.org/article/e6965916b8224b0991c682bb7bbd8be92021-10-01T00:00:00Zhttps://celljournal.org/journal/article/fulltext/circular-rna-has-circ-0000517-facilitates-the-growth-and-metastasis-in-non-small-cell-lung-cancer-by-sponging-mir-326mir-330-5p.pdfhttps://doaj.org/toc/2228-5806https://doaj.org/toc/2228-5814Objective: There is growing evidence showing that circular RNAs (circRNAs) are crucial regulators in modulating the biological behavior of tumors. This work is aimed to probe the role of circ_0000517 in non-small cell lung cancer (NSCLC) and to elucidate its mechanism of action. Materials and Methods: In this experimental study, the differentially expressed circRNAs in NSCLC were screened using the GEO database (GSE158695). Circ_0000517, miR-326, miR-330-5p, and MMP2 expression levels were determined by quantitative real-time polymerase chain reaction (qRT-PCR) analysis and Western blot. The proliferation, apoptosis, migration, and invasion of NSCLC cells were detected by CCK-8, flow cytometry, and transwell assays. RNA immunoprecipitation (RIP), RNA pull-down, and dual-luciferase reporter gene assays were performed to clarify the association between the circ_0000517 and miR-326/miR-330-5p. Results: Circ_0000517 was shown to be up-regulated in NSCLC tissues and cell lines. The up-regulation of circ_0000517 is closely associated with advanced clinical stage of cancer, lymph node metastasis, and poor prognosis in NSCLC patients. Circ_0000517 knockdown impeded the proliferation, migration, and invasion of NSCLC cells and enhanced their apoptosis. Mechanistically, circ_0000517 was demonstrated to up-regulate MMP2 expression via decoying miR-326 and miR-330-5p to facilitate the malignant biological behaviors of NSCLC cells. Conclusion: This work reveals that circ_0000517 is implicated in NSCLC cell growth and metastasis through the modulation of miR-326/miR-330-5p/MMP2, providing novel insights into the role of circRNAs in NSCLC progression.Qiyan TanChangyu LiuYing ShenTao HuangRoyan Institute (ACECR), Tehranarticlemir-326mir-330-5pmmp2non-small cell lung cancerMedicineRScienceQENCell Journal, Vol 23, Iss 5, Pp 552-561 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	mir-326 mir-330-5p mmp2 non-small cell lung cancer Medicine R Science Q
spellingShingle	mir-326 mir-330-5p mmp2 non-small cell lung cancer Medicine R Science Q Qiyan Tan Changyu Liu Ying Shen Tao Huang Circular RNA circ_0000517 Facilitates The Growth and Metastasis of Non-Small Cell Lung Cancer by Sponging miR-326/miR-330-5p
description	Objective: There is growing evidence showing that circular RNAs (circRNAs) are crucial regulators in modulating the biological behavior of tumors. This work is aimed to probe the role of circ_0000517 in non-small cell lung cancer (NSCLC) and to elucidate its mechanism of action. Materials and Methods: In this experimental study, the differentially expressed circRNAs in NSCLC were screened using the GEO database (GSE158695). Circ_0000517, miR-326, miR-330-5p, and MMP2 expression levels were determined by quantitative real-time polymerase chain reaction (qRT-PCR) analysis and Western blot. The proliferation, apoptosis, migration, and invasion of NSCLC cells were detected by CCK-8, flow cytometry, and transwell assays. RNA immunoprecipitation (RIP), RNA pull-down, and dual-luciferase reporter gene assays were performed to clarify the association between the circ_0000517 and miR-326/miR-330-5p. Results: Circ_0000517 was shown to be up-regulated in NSCLC tissues and cell lines. The up-regulation of circ_0000517 is closely associated with advanced clinical stage of cancer, lymph node metastasis, and poor prognosis in NSCLC patients. Circ_0000517 knockdown impeded the proliferation, migration, and invasion of NSCLC cells and enhanced their apoptosis. Mechanistically, circ_0000517 was demonstrated to up-regulate MMP2 expression via decoying miR-326 and miR-330-5p to facilitate the malignant biological behaviors of NSCLC cells. Conclusion: This work reveals that circ_0000517 is implicated in NSCLC cell growth and metastasis through the modulation of miR-326/miR-330-5p/MMP2, providing novel insights into the role of circRNAs in NSCLC progression.
format	article
author	Qiyan Tan Changyu Liu Ying Shen Tao Huang
author_facet	Qiyan Tan Changyu Liu Ying Shen Tao Huang
author_sort	Qiyan Tan
title	Circular RNA circ_0000517 Facilitates The Growth and Metastasis of Non-Small Cell Lung Cancer by Sponging miR-326/miR-330-5p
title_short	Circular RNA circ_0000517 Facilitates The Growth and Metastasis of Non-Small Cell Lung Cancer by Sponging miR-326/miR-330-5p
title_full	Circular RNA circ_0000517 Facilitates The Growth and Metastasis of Non-Small Cell Lung Cancer by Sponging miR-326/miR-330-5p
title_fullStr	Circular RNA circ_0000517 Facilitates The Growth and Metastasis of Non-Small Cell Lung Cancer by Sponging miR-326/miR-330-5p
title_full_unstemmed	Circular RNA circ_0000517 Facilitates The Growth and Metastasis of Non-Small Cell Lung Cancer by Sponging miR-326/miR-330-5p
title_sort	circular rna circ_0000517 facilitates the growth and metastasis of non-small cell lung cancer by sponging mir-326/mir-330-5p
publisher	Royan Institute (ACECR), Tehran
publishDate	2021
url	https://doaj.org/article/e6965916b8224b0991c682bb7bbd8be9
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_version_	1718443582700912640

Circular RNA circ_0000517 Facilitates The Growth and Metastasis of Non-Small Cell Lung Cancer by Sponging miR-326/miR-330-5p

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