Quantification of FRET-induced angular displacement by monitoring sensitized acceptor anisotropy using a dim fluorescent donor

The FRET efficiency usually predominantly depends on the proximity of donor and acceptor. Here the authors report an anisotropy-based mode of FRET detection, FRET-induced Angular Displacement Evaluation via Dim donor (FADED), to allow quantification of the relative angle between donor and acceptor.

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Autores principales:	Danai Laskaratou, Guillermo Solís Fernández, Quinten Coucke, Eduard Fron, Susana Rocha, Johan Hofkens, Jelle Hendrix, Hideaki Mizuno
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/e69808e0f8e94533a612962a43ef72cd
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spelling	oai:doaj.org-article:e69808e0f8e94533a612962a43ef72cd2021-12-02T14:49:23ZQuantification of FRET-induced angular displacement by monitoring sensitized acceptor anisotropy using a dim fluorescent donor10.1038/s41467-021-22816-72041-1723https://doaj.org/article/e69808e0f8e94533a612962a43ef72cd2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-22816-7https://doaj.org/toc/2041-1723The FRET efficiency usually predominantly depends on the proximity of donor and acceptor. Here the authors report an anisotropy-based mode of FRET detection, FRET-induced Angular Displacement Evaluation via Dim donor (FADED), to allow quantification of the relative angle between donor and acceptor.Danai LaskaratouGuillermo Solís FernándezQuinten CouckeEduard FronSusana RochaJohan HofkensJelle HendrixHideaki MizunoNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Danai Laskaratou Guillermo Solís Fernández Quinten Coucke Eduard Fron Susana Rocha Johan Hofkens Jelle Hendrix Hideaki Mizuno Quantification of FRET-induced angular displacement by monitoring sensitized acceptor anisotropy using a dim fluorescent donor
description	The FRET efficiency usually predominantly depends on the proximity of donor and acceptor. Here the authors report an anisotropy-based mode of FRET detection, FRET-induced Angular Displacement Evaluation via Dim donor (FADED), to allow quantification of the relative angle between donor and acceptor.
format	article
author	Danai Laskaratou Guillermo Solís Fernández Quinten Coucke Eduard Fron Susana Rocha Johan Hofkens Jelle Hendrix Hideaki Mizuno
author_facet	Danai Laskaratou Guillermo Solís Fernández Quinten Coucke Eduard Fron Susana Rocha Johan Hofkens Jelle Hendrix Hideaki Mizuno
author_sort	Danai Laskaratou
title	Quantification of FRET-induced angular displacement by monitoring sensitized acceptor anisotropy using a dim fluorescent donor
title_short	Quantification of FRET-induced angular displacement by monitoring sensitized acceptor anisotropy using a dim fluorescent donor
title_full	Quantification of FRET-induced angular displacement by monitoring sensitized acceptor anisotropy using a dim fluorescent donor
title_fullStr	Quantification of FRET-induced angular displacement by monitoring sensitized acceptor anisotropy using a dim fluorescent donor
title_full_unstemmed	Quantification of FRET-induced angular displacement by monitoring sensitized acceptor anisotropy using a dim fluorescent donor
title_sort	quantification of fret-induced angular displacement by monitoring sensitized acceptor anisotropy using a dim fluorescent donor
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e69808e0f8e94533a612962a43ef72cd
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Quantification of FRET-induced angular displacement by monitoring sensitized acceptor anisotropy using a dim fluorescent donor

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