Presenilin 2 overexpression is associated with apoptosis in Neuro2a cells

Presenilin 1 (PS1) and PS2 are evolutionarily conserved transmembrane proteins of the aspartyl protease family. Initially, they were reported to be associated with the early onset of familial, early-onset Alzheimer’s disease. PS1 has been implicated in several crucial brain functions including devel...

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Autores principales:	Kumar Ashish, Sivanandam T. M., Thakur M. K.
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2016
Materias:	chromatin condensation gene expression p53 presenilin Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
Acceso en línea:	https://doaj.org/article/e69aed8939f14bb49c66a358c80bf2f8
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spelling	oai:doaj.org-article:e69aed8939f14bb49c66a358c80bf2f82021-12-05T14:11:04ZPresenilin 2 overexpression is associated with apoptosis in Neuro2a cells2081-693610.1515/tnsci-2016-0011https://doaj.org/article/e69aed8939f14bb49c66a358c80bf2f82016-01-01T00:00:00Zhttps://doi.org/10.1515/tnsci-2016-0011https://doaj.org/toc/2081-6936Presenilin 1 (PS1) and PS2 are evolutionarily conserved transmembrane proteins of the aspartyl protease family. Initially, they were reported to be associated with the early onset of familial, early-onset Alzheimer’s disease. PS1 has been implicated in several crucial brain functions including developmental processes, synaptic plasticity, and processing of various molecules, while PS2 has been poorly studied and is considered to be a compensatory partner of PS1. Certain controversial reports have suggested that PS2 has a role in apoptosis, though the underlying mechanism is not clear. To ascertain the role of PS2 in apoptosis, mouse neuroblastoma cells (Neuro2a) were transfected with a cDNA construct encoding full length mouse PS2 and analyzed for viability, expression of PS1, PS2, Bax and p53, Bax protein, and status of chromatin condensation. Our results showed reduced viability, condensed chromatin and higher expression of Bax at mRNA and protein levels, but no change in the expression of p53 and PS1 in PS2-overexpressing Neuro2a cells. Thus, it is evident that PS2, independent of PS1, is associated with apoptosis via a Bax-mediated pathway. These findings might help in the understanding of the involvement of PS2 in apoptosis and its associated brain disorders.Kumar AshishSivanandam T. M.Thakur M. K.De Gruyterarticlechromatin condensationgene expressionp53presenilinNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571ENTranslational Neuroscience, Vol 7, Iss 1, Pp 71-75 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	chromatin condensation gene expression p53 presenilin Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
spellingShingle	chromatin condensation gene expression p53 presenilin Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Kumar Ashish Sivanandam T. M. Thakur M. K. Presenilin 2 overexpression is associated with apoptosis in Neuro2a cells
description	Presenilin 1 (PS1) and PS2 are evolutionarily conserved transmembrane proteins of the aspartyl protease family. Initially, they were reported to be associated with the early onset of familial, early-onset Alzheimer’s disease. PS1 has been implicated in several crucial brain functions including developmental processes, synaptic plasticity, and processing of various molecules, while PS2 has been poorly studied and is considered to be a compensatory partner of PS1. Certain controversial reports have suggested that PS2 has a role in apoptosis, though the underlying mechanism is not clear. To ascertain the role of PS2 in apoptosis, mouse neuroblastoma cells (Neuro2a) were transfected with a cDNA construct encoding full length mouse PS2 and analyzed for viability, expression of PS1, PS2, Bax and p53, Bax protein, and status of chromatin condensation. Our results showed reduced viability, condensed chromatin and higher expression of Bax at mRNA and protein levels, but no change in the expression of p53 and PS1 in PS2-overexpressing Neuro2a cells. Thus, it is evident that PS2, independent of PS1, is associated with apoptosis via a Bax-mediated pathway. These findings might help in the understanding of the involvement of PS2 in apoptosis and its associated brain disorders.
format	article
author	Kumar Ashish Sivanandam T. M. Thakur M. K.
author_facet	Kumar Ashish Sivanandam T. M. Thakur M. K.
author_sort	Kumar Ashish
title	Presenilin 2 overexpression is associated with apoptosis in Neuro2a cells
title_short	Presenilin 2 overexpression is associated with apoptosis in Neuro2a cells
title_full	Presenilin 2 overexpression is associated with apoptosis in Neuro2a cells
title_fullStr	Presenilin 2 overexpression is associated with apoptosis in Neuro2a cells
title_full_unstemmed	Presenilin 2 overexpression is associated with apoptosis in Neuro2a cells
title_sort	presenilin 2 overexpression is associated with apoptosis in neuro2a cells
publisher	De Gruyter
publishDate	2016
url	https://doaj.org/article/e69aed8939f14bb49c66a358c80bf2f8
work_keys_str_mv	AT kumarashish presenilin2overexpressionisassociatedwithapoptosisinneuro2acells AT sivanandamtm presenilin2overexpressionisassociatedwithapoptosisinneuro2acells AT thakurmk presenilin2overexpressionisassociatedwithapoptosisinneuro2acells
_version_	1718371451070840832

Presenilin 2 overexpression is associated with apoptosis in Neuro2a cells

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