An improved protocol for intact chloroplasts and cpDNA isolation in conifers.

An improved protocol for intact chloroplasts and cpDNA isolation in conifers.

<h4>Background</h4>Performing chloroplast DNA (cpDNA) isolation is considered a major challenge among different plant groups, especially conifers. Isolating chloroplasts in conifers by such conventional methods as sucrose gradient and high salt has not been successful. So far, plastid ge...

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Autores principales: Leila do Nascimento Vieira, Helisson Faoro, Hugo Pacheco de Freitas Fraga, Marcelo Rogalski, Emanuel Maltempi de Souza, Fábio de Oliveira Pedrosa, Rubens Onofre Nodari, Miguel Pedro Guerra
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2014
Materias:
Medicine
R
Science
Q
Acceso en línea:https://doaj.org/article/e69c8779d2774cc599b40704ccc3c7e4
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