DMF-MALDI: droplet based microfluidic combined to MALDI-TOF for focused peptide detection

Abstract We present an automated droplet microfluidic system (DMF) to generate monitored nanoliter aqueous droplets in oil and their deposition on a commercial stainless steel plate for MALDI-TOF analysis of peptides or protein digests. We demonstrate that DMF-MALDI combination focuses the analyte o...

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Autores principales:	Kiarach Mesbah, Robert Thai, Sarah Bregant, Florent Malloggi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e69f693adbf54fc9b48ca747cf076df1
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spelling	oai:doaj.org-article:e69f693adbf54fc9b48ca747cf076df12021-12-02T11:52:59ZDMF-MALDI: droplet based microfluidic combined to MALDI-TOF for focused peptide detection10.1038/s41598-017-06660-82045-2322https://doaj.org/article/e69f693adbf54fc9b48ca747cf076df12017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06660-8https://doaj.org/toc/2045-2322Abstract We present an automated droplet microfluidic system (DMF) to generate monitored nanoliter aqueous droplets in oil and their deposition on a commercial stainless steel plate for MALDI-TOF analysis of peptides or protein digests. We demonstrate that DMF-MALDI combination focuses the analyte on the MALDI plate, increasing considerably the homogeneity of the dried material. This results in a 30times enhanced MALDI-TOF MS signal for a model peptide, allowing a significant improvement of the detection sensitivity limit (down to few tens of attomoles). Moreover, positive detection can be achieved from sub-nanomolar peptides solutions and better overall protein sequence coverages are obtained from few tens attomoles of protein digest. These results make DMF-MALDI a promising approach for the treatment of peptides samples as well as a key component for an integrated approach in the proteomic field.Kiarach MesbahRobert ThaiSarah BregantFlorent MalloggiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kiarach Mesbah Robert Thai Sarah Bregant Florent Malloggi DMF-MALDI: droplet based microfluidic combined to MALDI-TOF for focused peptide detection
description	Abstract We present an automated droplet microfluidic system (DMF) to generate monitored nanoliter aqueous droplets in oil and their deposition on a commercial stainless steel plate for MALDI-TOF analysis of peptides or protein digests. We demonstrate that DMF-MALDI combination focuses the analyte on the MALDI plate, increasing considerably the homogeneity of the dried material. This results in a 30times enhanced MALDI-TOF MS signal for a model peptide, allowing a significant improvement of the detection sensitivity limit (down to few tens of attomoles). Moreover, positive detection can be achieved from sub-nanomolar peptides solutions and better overall protein sequence coverages are obtained from few tens attomoles of protein digest. These results make DMF-MALDI a promising approach for the treatment of peptides samples as well as a key component for an integrated approach in the proteomic field.
format	article
author	Kiarach Mesbah Robert Thai Sarah Bregant Florent Malloggi
author_facet	Kiarach Mesbah Robert Thai Sarah Bregant Florent Malloggi
author_sort	Kiarach Mesbah
title	DMF-MALDI: droplet based microfluidic combined to MALDI-TOF for focused peptide detection
title_short	DMF-MALDI: droplet based microfluidic combined to MALDI-TOF for focused peptide detection
title_full	DMF-MALDI: droplet based microfluidic combined to MALDI-TOF for focused peptide detection
title_fullStr	DMF-MALDI: droplet based microfluidic combined to MALDI-TOF for focused peptide detection
title_full_unstemmed	DMF-MALDI: droplet based microfluidic combined to MALDI-TOF for focused peptide detection
title_sort	dmf-maldi: droplet based microfluidic combined to maldi-tof for focused peptide detection
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/e69f693adbf54fc9b48ca747cf076df1
work_keys_str_mv	AT kiarachmesbah dmfmaldidropletbasedmicrofluidiccombinedtomalditofforfocusedpeptidedetection AT robertthai dmfmaldidropletbasedmicrofluidiccombinedtomalditofforfocusedpeptidedetection AT sarahbregant dmfmaldidropletbasedmicrofluidiccombinedtomalditofforfocusedpeptidedetection AT florentmalloggi dmfmaldidropletbasedmicrofluidiccombinedtomalditofforfocusedpeptidedetection
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DMF-MALDI: droplet based microfluidic combined to MALDI-TOF for focused peptide detection

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