Fluorescent nanosensors reveal dynamic pH gradients during biofilm formation

Abstract Understanding the dynamic environmental microniches of biofilms will permit us to detect, manage and exploit these communities. The components and architecture of biofilms have been interrogated in depth; however, little is known about the environmental microniches present. This is primaril...

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Autores principales:	Birte Hollmann, Mark Perkins, Veeren M. Chauhan, Jonathan W. Aylott, Kim R. Hardie
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Microbial ecology QR100-130
Acceso en línea:	https://doaj.org/article/e6a36964bbbb4827b583c3689a6a2b9d
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spelling	oai:doaj.org-article:e6a36964bbbb4827b583c3689a6a2b9d2021-12-02T17:41:32ZFluorescent nanosensors reveal dynamic pH gradients during biofilm formation10.1038/s41522-021-00221-82055-5008https://doaj.org/article/e6a36964bbbb4827b583c3689a6a2b9d2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41522-021-00221-8https://doaj.org/toc/2055-5008Abstract Understanding the dynamic environmental microniches of biofilms will permit us to detect, manage and exploit these communities. The components and architecture of biofilms have been interrogated in depth; however, little is known about the environmental microniches present. This is primarily because of the absence of tools with the required measurement sensitivity and resolution to detect these changes. We describe the application of ratiometric fluorescent pH-sensitive nanosensors, as a tool, to observe physiological pH changes in biofilms in real time. Nanosensors comprised two pH-sensitive fluorophores covalently encapsulated with a reference pH-insensitive fluorophore in an inert polyacrylamide nanoparticle matrix. The nanosensors were used to analyse the real-time three-dimensional pH variation for two model biofilm formers: (i) opportunistic pathogen Pseudomonas aeruginosa and (ii) oral pathogen Streptococcus mutans. The detection of sugar metabolism in real time by nanosensors provides a potential application to identify therapeutic solutions to improve oral health.Birte HollmannMark PerkinsVeeren M. ChauhanJonathan W. AylottKim R. HardieNature PortfolioarticleMicrobial ecologyQR100-130ENnpj Biofilms and Microbiomes, Vol 7, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbial ecology QR100-130
spellingShingle	Microbial ecology QR100-130 Birte Hollmann Mark Perkins Veeren M. Chauhan Jonathan W. Aylott Kim R. Hardie Fluorescent nanosensors reveal dynamic pH gradients during biofilm formation
description	Abstract Understanding the dynamic environmental microniches of biofilms will permit us to detect, manage and exploit these communities. The components and architecture of biofilms have been interrogated in depth; however, little is known about the environmental microniches present. This is primarily because of the absence of tools with the required measurement sensitivity and resolution to detect these changes. We describe the application of ratiometric fluorescent pH-sensitive nanosensors, as a tool, to observe physiological pH changes in biofilms in real time. Nanosensors comprised two pH-sensitive fluorophores covalently encapsulated with a reference pH-insensitive fluorophore in an inert polyacrylamide nanoparticle matrix. The nanosensors were used to analyse the real-time three-dimensional pH variation for two model biofilm formers: (i) opportunistic pathogen Pseudomonas aeruginosa and (ii) oral pathogen Streptococcus mutans. The detection of sugar metabolism in real time by nanosensors provides a potential application to identify therapeutic solutions to improve oral health.
format	article
author	Birte Hollmann Mark Perkins Veeren M. Chauhan Jonathan W. Aylott Kim R. Hardie
author_facet	Birte Hollmann Mark Perkins Veeren M. Chauhan Jonathan W. Aylott Kim R. Hardie
author_sort	Birte Hollmann
title	Fluorescent nanosensors reveal dynamic pH gradients during biofilm formation
title_short	Fluorescent nanosensors reveal dynamic pH gradients during biofilm formation
title_full	Fluorescent nanosensors reveal dynamic pH gradients during biofilm formation
title_fullStr	Fluorescent nanosensors reveal dynamic pH gradients during biofilm formation
title_full_unstemmed	Fluorescent nanosensors reveal dynamic pH gradients during biofilm formation
title_sort	fluorescent nanosensors reveal dynamic ph gradients during biofilm formation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e6a36964bbbb4827b583c3689a6a2b9d
work_keys_str_mv	AT birtehollmann fluorescentnanosensorsrevealdynamicphgradientsduringbiofilmformation AT markperkins fluorescentnanosensorsrevealdynamicphgradientsduringbiofilmformation AT veerenmchauhan fluorescentnanosensorsrevealdynamicphgradientsduringbiofilmformation AT jonathanwaylott fluorescentnanosensorsrevealdynamicphgradientsduringbiofilmformation AT kimrhardie fluorescentnanosensorsrevealdynamicphgradientsduringbiofilmformation
_version_	1718379677885661184

Fluorescent nanosensors reveal dynamic pH gradients during biofilm formation

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