Characteristics of coagulase positive staphylococci isolated from milk in cases of subclinical mastitis

Coagulase-positive staphylococci are the most common pathogen causing subclinical mastitis in cows. Their main characteristic is a high virulence which leads to chronic infection. A total of 213 isolates of coagulase-positive staphylococci were tested. The majority of isolates (58%) formed a gold pi...

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Autores principales:	Rajić Savić Nataša, Katić Vera, Velebit Branko
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2014
Materias:	coagulase positive staphylococci hemolysis identification methicillin penicillin pigment Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/e6aca130bbfa400c91bb41dc24fc54d9
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spelling	oai:doaj.org-article:e6aca130bbfa400c91bb41dc24fc54d92021-11-17T21:27:52ZCharacteristics of coagulase positive staphylococci isolated from milk in cases of subclinical mastitis1820-744810.2478/acve-2014-0012https://doaj.org/article/e6aca130bbfa400c91bb41dc24fc54d92014-03-01T00:00:00Zhttps://doi.org/10.2478/acve-2014-0012https://doaj.org/toc/1820-7448Coagulase-positive staphylococci are the most common pathogen causing subclinical mastitis in cows. Their main characteristic is a high virulence which leads to chronic infection. A total of 213 isolates of coagulase-positive staphylococci were tested. The majority of isolates (58%) formed a gold pigment, then light gold (28%), white gold (8%), golden gray, creamy white and white (2%). The majority of isolated coagulase-positive staphylococci produce beta hemolysis on esculin blood agar (50%), alpha and beta hemolysis (36%), beta and delta hemolysis (8%), delta hemolysis (4%), and alpha hemolysis (2%). Biochemical and molecular identification was performed by APISTAPH and multiplex PCR method. The majority of isolates were identified as S. aureus (88%), S. chromogenes (4%), and 2% of the isolates were identified as S. lentus, S. sciuri, S. xylosus, S. intermedius by APISTAPH. Antimicrobial susceptibility to penicillin G, was found by the Kirby Bauer disk diffusion method to be resistant (62.44%). For disc diffusion penicillin G sensitive isolates the minimal inhibitory concentration (MIC) was established for MIC50 and MIC90 as 0.003 mg/ml and 2μg/ml, respectively. For disc diffusion penicillin G resistant isolates MIC50 and MIC90 was 1.0μg/ml and 16μg/ ml, respectively. The study of phenotypic resistance to methicillin, as recommended by CLSI, established resistance to oxacillin in 5.26% of the isolates, while no resistance was found to cefoxitine. None of the tested isolates have the mecA gene.Rajić Savić NatašaKatić VeraVelebit BrankoSciendoarticlecoagulase positive staphylococcihemolysisidentificationmethicillinpenicillinpigmentVeterinary medicineSF600-1100ENActa Veterinaria, Vol 64, Iss 1, Pp 115-123 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	coagulase positive staphylococci hemolysis identification methicillin penicillin pigment Veterinary medicine SF600-1100
spellingShingle	coagulase positive staphylococci hemolysis identification methicillin penicillin pigment Veterinary medicine SF600-1100 Rajić Savić Nataša Katić Vera Velebit Branko Characteristics of coagulase positive staphylococci isolated from milk in cases of subclinical mastitis
description	Coagulase-positive staphylococci are the most common pathogen causing subclinical mastitis in cows. Their main characteristic is a high virulence which leads to chronic infection. A total of 213 isolates of coagulase-positive staphylococci were tested. The majority of isolates (58%) formed a gold pigment, then light gold (28%), white gold (8%), golden gray, creamy white and white (2%). The majority of isolated coagulase-positive staphylococci produce beta hemolysis on esculin blood agar (50%), alpha and beta hemolysis (36%), beta and delta hemolysis (8%), delta hemolysis (4%), and alpha hemolysis (2%). Biochemical and molecular identification was performed by APISTAPH and multiplex PCR method. The majority of isolates were identified as S. aureus (88%), S. chromogenes (4%), and 2% of the isolates were identified as S. lentus, S. sciuri, S. xylosus, S. intermedius by APISTAPH. Antimicrobial susceptibility to penicillin G, was found by the Kirby Bauer disk diffusion method to be resistant (62.44%). For disc diffusion penicillin G sensitive isolates the minimal inhibitory concentration (MIC) was established for MIC50 and MIC90 as 0.003 mg/ml and 2μg/ml, respectively. For disc diffusion penicillin G resistant isolates MIC50 and MIC90 was 1.0μg/ml and 16μg/ ml, respectively. The study of phenotypic resistance to methicillin, as recommended by CLSI, established resistance to oxacillin in 5.26% of the isolates, while no resistance was found to cefoxitine. None of the tested isolates have the mecA gene.
format	article
author	Rajić Savić Nataša Katić Vera Velebit Branko
author_facet	Rajić Savić Nataša Katić Vera Velebit Branko
author_sort	Rajić Savić Nataša
title	Characteristics of coagulase positive staphylococci isolated from milk in cases of subclinical mastitis
title_short	Characteristics of coagulase positive staphylococci isolated from milk in cases of subclinical mastitis
title_full	Characteristics of coagulase positive staphylococci isolated from milk in cases of subclinical mastitis
title_fullStr	Characteristics of coagulase positive staphylococci isolated from milk in cases of subclinical mastitis
title_full_unstemmed	Characteristics of coagulase positive staphylococci isolated from milk in cases of subclinical mastitis
title_sort	characteristics of coagulase positive staphylococci isolated from milk in cases of subclinical mastitis
publisher	Sciendo
publishDate	2014
url	https://doaj.org/article/e6aca130bbfa400c91bb41dc24fc54d9
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Characteristics of coagulase positive staphylococci isolated from milk in cases of subclinical mastitis

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