Comparative study of antioxidant and anticancer activities and HPTLC quantification of rutin in white radish (Raphanus sativus L.) leaves and root extracts grown in Saudi Arabia

The nutrient contents of Raphanus sativus L. (white radish) leaves (RSLs) and roots are known to have promising vital effects. We comparatively investigated the leaves and roots of R. sativus grown in Saudi Arabia to estimate the total phenol and flavonoid contents using the standard colorimetric me...

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Autores principales:	Noman Omar M., Nasr Fahd A., Alqahtani Ali S., Al-zharani Mohammed, Cordero Mary Anne W., Alotaibi Amal A., Bepari Asmatanzeem, Alarifi Saud, Daoud Ali
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	raphanus sativus rutin hptl antioxidant anticancer Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/e6bb21791c7443fdab8b27d7e23f4417
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spelling	oai:doaj.org-article:e6bb21791c7443fdab8b27d7e23f44172021-12-05T14:10:44ZComparative study of antioxidant and anticancer activities and HPTLC quantification of rutin in white radish (Raphanus sativus L.) leaves and root extracts grown in Saudi Arabia2391-542010.1515/chem-2021-0042https://doaj.org/article/e6bb21791c7443fdab8b27d7e23f44172021-03-01T00:00:00Zhttps://doi.org/10.1515/chem-2021-0042https://doaj.org/toc/2391-5420The nutrient contents of Raphanus sativus L. (white radish) leaves (RSLs) and roots are known to have promising vital effects. We comparatively investigated the leaves and roots of R. sativus grown in Saudi Arabia to estimate the total phenol and flavonoid contents using the standard colorimetric methods. The antioxidant activity of RSLs and R. sativus roots (RSRs) were measured by 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays. Both extracts’ cytotoxic activity was assessed by MTT assay against several human cancer cell lines (A549, HepG2, MDA-MB-231, and MCF-7). High-performance thin-layer chromatography was used to identify and quantify the biomarker compound rutin in both extracts. The total phenolic content in RSLs (125.3 mg of gallic acid equivalent [GAE]/g) was higher than that in roots (95.8 of GAE/g), whereas the flavonoid content (44.5 mg of quercetin equivalent [QE]/g) was almost double compared in RSR extract (24.4 of QE/g). Similarly, RSLs exhibited a higher antioxidative activity than RSRs in both DPPH (IC50 216.8 vs 359.7 µg/mL) and ABTS (IC50 326.7 vs 549 µg/mL) models. RSLs also demonstrated the highest antiproliferative efficiency against all cell lines, with IC50 values of 217–453 µg/mL. The reversed-phase-high-performance thin-layer chromatography results showed the presence of rutin (5.2 µg/mg) only in RSLs. Our study indicates RSLs as a promising source of bioactive compounds compared with roots.Noman Omar M.Nasr Fahd A.Alqahtani Ali S.Al-zharani MohammedCordero Mary Anne W.Alotaibi Amal A.Bepari AsmatanzeemAlarifi SaudDaoud AliDe Gruyterarticleraphanus sativusrutinhptlantioxidantanticancerChemistryQD1-999ENOpen Chemistry, Vol 19, Iss 1, Pp 408-416 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	raphanus sativus rutin hptl antioxidant anticancer Chemistry QD1-999
spellingShingle	raphanus sativus rutin hptl antioxidant anticancer Chemistry QD1-999 Noman Omar M. Nasr Fahd A. Alqahtani Ali S. Al-zharani Mohammed Cordero Mary Anne W. Alotaibi Amal A. Bepari Asmatanzeem Alarifi Saud Daoud Ali Comparative study of antioxidant and anticancer activities and HPTLC quantification of rutin in white radish (Raphanus sativus L.) leaves and root extracts grown in Saudi Arabia
description	The nutrient contents of Raphanus sativus L. (white radish) leaves (RSLs) and roots are known to have promising vital effects. We comparatively investigated the leaves and roots of R. sativus grown in Saudi Arabia to estimate the total phenol and flavonoid contents using the standard colorimetric methods. The antioxidant activity of RSLs and R. sativus roots (RSRs) were measured by 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays. Both extracts’ cytotoxic activity was assessed by MTT assay against several human cancer cell lines (A549, HepG2, MDA-MB-231, and MCF-7). High-performance thin-layer chromatography was used to identify and quantify the biomarker compound rutin in both extracts. The total phenolic content in RSLs (125.3 mg of gallic acid equivalent [GAE]/g) was higher than that in roots (95.8 of GAE/g), whereas the flavonoid content (44.5 mg of quercetin equivalent [QE]/g) was almost double compared in RSR extract (24.4 of QE/g). Similarly, RSLs exhibited a higher antioxidative activity than RSRs in both DPPH (IC50 216.8 vs 359.7 µg/mL) and ABTS (IC50 326.7 vs 549 µg/mL) models. RSLs also demonstrated the highest antiproliferative efficiency against all cell lines, with IC50 values of 217–453 µg/mL. The reversed-phase-high-performance thin-layer chromatography results showed the presence of rutin (5.2 µg/mg) only in RSLs. Our study indicates RSLs as a promising source of bioactive compounds compared with roots.
format	article
author	Noman Omar M. Nasr Fahd A. Alqahtani Ali S. Al-zharani Mohammed Cordero Mary Anne W. Alotaibi Amal A. Bepari Asmatanzeem Alarifi Saud Daoud Ali
author_facet	Noman Omar M. Nasr Fahd A. Alqahtani Ali S. Al-zharani Mohammed Cordero Mary Anne W. Alotaibi Amal A. Bepari Asmatanzeem Alarifi Saud Daoud Ali
author_sort	Noman Omar M.
title	Comparative study of antioxidant and anticancer activities and HPTLC quantification of rutin in white radish (Raphanus sativus L.) leaves and root extracts grown in Saudi Arabia
title_short	Comparative study of antioxidant and anticancer activities and HPTLC quantification of rutin in white radish (Raphanus sativus L.) leaves and root extracts grown in Saudi Arabia
title_full	Comparative study of antioxidant and anticancer activities and HPTLC quantification of rutin in white radish (Raphanus sativus L.) leaves and root extracts grown in Saudi Arabia
title_fullStr	Comparative study of antioxidant and anticancer activities and HPTLC quantification of rutin in white radish (Raphanus sativus L.) leaves and root extracts grown in Saudi Arabia
title_full_unstemmed	Comparative study of antioxidant and anticancer activities and HPTLC quantification of rutin in white radish (Raphanus sativus L.) leaves and root extracts grown in Saudi Arabia
title_sort	comparative study of antioxidant and anticancer activities and hptlc quantification of rutin in white radish (raphanus sativus l.) leaves and root extracts grown in saudi arabia
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/e6bb21791c7443fdab8b27d7e23f4417
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Comparative study of antioxidant and anticancer activities and HPTLC quantification of rutin in white radish (Raphanus sativus L.) leaves and root extracts grown in Saudi Arabia

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